Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 953,232 A→G 8.9% H13R (CAC→CGC) ‡ cyoC → cytochrome bo terminal oxidase subunit III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947953,2320AG 8.9% 125.9 / 3.0 45H13R (CAC→CGC) ‡cyoCcytochrome bo terminal oxidase subunit III
Reads supporting (aligned to +/- strand):  ref base A (18/23);  new base G (2/2);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

GGTTCCGGCCGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGCGGCCC  >  NC_002947/953083‑953376
                                                                                                                                                     |                                                                                                                                                
ggTTCCGGCCGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGCTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCa                                                                                                                                                  <  2:271812/150‑1 (MQ=255)
  ttCCGGCCGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACg                                                                                                                                                >  2:491979/1‑150 (MQ=255)
      ggCCGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCa                                                                                                                                            >  1:582713/1‑150 (MQ=255)
         cGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTATGCCTTCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTTAAGTAATGCACGGCGCTGCTCATGGTCACGACCATgg                                                                                                                                         <  2:347326/150‑1 (MQ=255)
          gaagaaGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATggg                                                                                                                                        <  2:194431/150‑1 (MQ=255)
          gaagaaGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATggg                                                                                                                                        >  1:418036/1‑150 (MQ=255)
                         aTCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGaccaccac                                                                                                                         >  1:555288/1‑150 (MQ=255)
                           cGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGAccaccacca                                                                                                                       <  1:94233/150‑1 (MQ=255)
                              aGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGTACGTGTCGAATCGTTTGAGCGGGTGTAATCAATTTCCAGTCAAGTAATGCCCCGCCCTGCTCATGGTCACTACCATGGGCATGACCACCACCACCACGa                                                                                                                    <  2:313770/150‑1 (MQ=255)
                              aGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGa                                                                                                                    <  2:658940/150‑1 (MQ=255)
                              aGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGa                                                                                                                    >  2:693860/1‑150 (MQ=255)
                                  gAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATgacgac                                                                                                                               >  2:529086/1‑135 (MQ=255)
                                  gAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATgacgac                                                                                                                               <  1:529086/135‑1 (MQ=255)
                                   aGCGTATGAAAGCGCTGGCAAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCgg                                                                                                               <  2:582713/150‑1 (MQ=255)
                                           aaaGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATg                                                                                                       >  2:673617/1‑150 (MQ=255)
                                                    gCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTg                                                                                              <  1:312471/150‑1 (MQ=255)
                                                      cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCCCATGGTCGTGACCATGAGCagc                                                                                                                                    <  2:128354/110‑3 (MQ=255)
                                                      cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCCCATGGTCGTGACCATGAGCagc                                                                                                                                    <  2:128292/110‑3 (MQ=255)
                                                      cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCCCATGGTCGTGACCATGAGCagc                                                                                                                                    >  1:128354/1‑108 (MQ=255)
                                                      cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCCCATGGTCGTGACCATGAGCagc                                                                                                                                    >  1:128292/1‑108 (MQ=255)
                                                      cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCCGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTggg                                                                                            >  2:657451/1‑150 (MQ=255)
                                                           cAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCt                                                                                       <  2:576696/150‑1 (MQ=255)
                                                           cAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCt                                                                                       <  2:555288/150‑1 (MQ=255)
                                                              gTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGc                                                                                    <  2:499203/150‑1 (MQ=255)
                                                                  tctGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGc                                                                                                                                       <  2:507235/95‑1 (MQ=255)
                                                                  tctGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGc                                                                                                                                       >  1:507235/1‑95 (MQ=255)
                                                                        tgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCtgat                                                                          <  1:693860/150‑1 (MQ=255)
                                                                              cgcACGTGTCGCATCGTTTGCGCGGGTGTAATCAACGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGa                                                                    <  1:713479/150‑1 (MQ=255)
                                                                                cACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACt                                                                  <  1:611050/150‑1 (MQ=255)
                                                                                       cGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCt                                                           <  1:688142/150‑1 (MQ=255)
                                                                                         aaTCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGt                                                         <  2:418036/150‑1 (MQ=255)
                                                                                          aTCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCgg                                                                                                               >  2:305190/1‑95 (MQ=255)
                                                                                          aTCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCgg                                                                                                               <  1:305190/95‑1 (MQ=255)
                                                                                           tCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTc                                                                                        >  2:693204/1‑117 (MQ=255)
                                                                                           tCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTc                                                                                        <  1:693204/117‑1 (MQ=255)
                                                                                             gTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCa                                                                                                                                            <  1:242776/63‑1 (MQ=255)
                                                                                             gTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCa                                                                                                                                            >  2:242776/1‑63 (MQ=255)
                                                                                                             tCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGt                                                         <  2:330523/130‑1 (MQ=255)
                                                                                                             tCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGt                                                         >  1:330523/1‑130 (MQ=255)
                                                                                                                      aGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGccacccaccacgtg                            >  2:399191/1‑144 (MQ=255)
                                                                                                                        tCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTc                                           >  2:215904/1‑133 (MQ=255)
                                                                                                                        tCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTc                                           <  1:215904/133‑1 (MQ=255)
                                                                                                                               aTGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGAGTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGc                   >  2:535639/1‑150 (MQ=255)
                                                                                                                               aTGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGc                   >  2:683992/1‑150 (MQ=255)
                                                                                                                                       cgcTGCTCATGGTCACGTTGCCTGTCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTCCCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGc           <  1:243049/150‑1 (MQ=255)
                                                                                                                                                tcgTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGCGGccc  >  1:468866/3‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GGTTCCGGCCGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGCGGCCC  >  NC_002947/953083‑953376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑