Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 953,233 C→T 8.9% H13H (CAC→CAT) ‡ cyoC → cytochrome bo terminal oxidase subunit III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947953,2330CT 8.9% 123.5 / 2.5 45H13H (CAC→CAT) ‡cyoCcytochrome bo terminal oxidase subunit III
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base T (2/2);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

TTCCGGCCGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGCGGCCCGTCGGG  >  NC_002947/953085‑953382
                                                                                                                                                    |                                                                                                                                                     
ttCCGGCCGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACg                                                                                                                                                      >  2:491979/1‑150 (MQ=255)
    ggCCGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCa                                                                                                                                                  >  1:582713/1‑150 (MQ=255)
       cGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTATGCCTTCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTTAAGTAATGCACGGCGCTGCTCATGGTCACGACCATgg                                                                                                                                               <  2:347326/150‑1 (MQ=255)
        gaagaaGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATggg                                                                                                                                              <  2:194431/150‑1 (MQ=255)
        gaagaaGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATggg                                                                                                                                              >  1:418036/1‑150 (MQ=255)
                       aTCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGaccaccac                                                                                                                               >  1:555288/1‑150 (MQ=255)
                         cGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGAccaccacca                                                                                                                             <  1:94233/150‑1 (MQ=255)
                            aGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGTACGTGTCGAATCGTTTGAGCGGGTGTAATCAATTTCCAGTCAAGTAATGCCCCGCCCTGCTCATGGTCACTACCATGGGCATGACCACCACCACCACGa                                                                                                                          <  2:313770/150‑1 (MQ=255)
                            aGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGa                                                                                                                          >  2:693860/1‑150 (MQ=255)
                            aGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGa                                                                                                                          <  2:658940/150‑1 (MQ=255)
                                gAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATgacgac                                                                                                                                     >  2:529086/1‑135 (MQ=255)
                                gAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATgacgac                                                                                                                                     <  1:529086/135‑1 (MQ=255)
                                 aGCGTATGAAAGCGCTGGCAAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCgg                                                                                                                     <  2:582713/150‑1 (MQ=255)
                                         aaaGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATg                                                                                                             >  2:673617/1‑150 (MQ=255)
                                                  gCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTg                                                                                                    <  1:312471/150‑1 (MQ=255)
                                                    cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCCCATGGTCGTGACCATGAGCagc                                                                                                                                          >  1:128292/1‑108 (MQ=255)
                                                    cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCCCATGGTCGTGACCATGAGCagc                                                                                                                                          <  2:128354/110‑3 (MQ=255)
                                                    cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCCCATGGTCGTGACCATGAGCagc                                                                                                                                          <  2:128292/110‑3 (MQ=255)
                                                    cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCCCATGGTCGTGACCATGAGCagc                                                                                                                                          >  1:128354/1‑108 (MQ=255)
                                                    cAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCCGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTggg                                                                                                  >  2:657451/1‑150 (MQ=255)
                                                         cAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCt                                                                                             <  2:576696/150‑1 (MQ=255)
                                                         cAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCt                                                                                             <  2:555288/150‑1 (MQ=255)
                                                            gTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGc                                                                                          <  2:499203/150‑1 (MQ=255)
                                                                tctGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGc                                                                                                                                             <  2:507235/95‑1 (MQ=255)
                                                                tctGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGc                                                                                                                                             >  1:507235/1‑95 (MQ=255)
                                                                      tgccGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCtgat                                                                                <  1:693860/150‑1 (MQ=255)
                                                                            cgcACGTGTCGCATCGTTTGCGCGGGTGTAATCAACGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGa                                                                          <  1:713479/150‑1 (MQ=255)
                                                                              cACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACt                                                                        <  1:611050/150‑1 (MQ=255)
                                                                                     cGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCt                                                                 <  1:688142/150‑1 (MQ=255)
                                                                                       aaTCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGt                                                               <  2:418036/150‑1 (MQ=255)
                                                                                        aTCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCgg                                                                                                                     >  2:305190/1‑95 (MQ=255)
                                                                                        aTCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCgg                                                                                                                     <  1:305190/95‑1 (MQ=255)
                                                                                         tCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTc                                                                                              >  2:693204/1‑117 (MQ=255)
                                                                                         tCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTc                                                                                              <  1:693204/117‑1 (MQ=255)
                                                                                           gTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCa                                                                                                                                                  >  2:242776/1‑63 (MQ=255)
                                                                                           gTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCa                                                                                                                                                  <  1:242776/63‑1 (MQ=255)
                                                                                                           tCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGt                                                               <  2:330523/130‑1 (MQ=255)
                                                                                                           tCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGt                                                               >  1:330523/1‑130 (MQ=255)
                                                                                                                    aGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGccacccaccacgtg                                  >  2:399191/1‑144 (MQ=255)
                                                                                                                      tCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTc                                                 >  2:215904/1‑133 (MQ=255)
                                                                                                                      tCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTc                                                 <  1:215904/133‑1 (MQ=255)
                                                                                                                             aTGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGAGTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGc                         >  2:535639/1‑150 (MQ=255)
                                                                                                                             aTGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGc                         >  2:683992/1‑150 (MQ=255)
                                                                                                                                     cgcTGCTCATGGTCACGTTGCCTGTCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTCCCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGc                 <  1:243049/150‑1 (MQ=255)
                                                                                                                                              tcgTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGCGGccc        >  1:468866/3‑150 (MQ=255)
                                                                                                                                                    cGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGAGCCTCTGGCTGTACCTGATGACCGACTGCCTCCTGTTTGCCGCGCTCCTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGCCGGCCCGtggcg  >  2:74941/1‑146 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTCCGGCCGAAGAAGTGGCGCGCATCGAAGGCGAGCGTATGAAAGCGCTGGCCAAAGCAGGTGCTCTGCCTGCCGGCGCACGTGTCGAATCGTTTGAGCGGGTGTAATCAATGTCCAGTCAAGTAATGCACGGCGCTGCTCATGGTCACGACCATGGGCATGACGACCACCACCACGACTCGGGCCAGATGACCGTACTGGGCTTCTGGCTGTACCTGATGACCGACTGCATCCTGTTTGCGTCGCTCTTCGCCACCTACGCGGTGCTGTCCGGCAGTTTTGCCGGCGGCCCGTCGGG  >  NC_002947/953085‑953382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑