| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 972,118 | T→A | 7.7% | L136Q (CTG→CAG) | secD → | protein translocase subunit SecD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 972,118 | 0 | T | A | 7.7% | 152.4 / 4.2 | 52 | L136Q (CTG→CAG) | secD | protein translocase subunit SecD |
| Reads supporting (aligned to +/- strand): ref base T (22/26); new base A (2/2); total (24/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
CAGCCAAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGACGAT > NC_002947/971974‑972263 | cagccaAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTgg > 2:556545/1‑150 (MQ=255) gccgAGGATGTAGTTCGCAAGGCAGTGGGCGAGGATTACGTCGTGGCCCTTAACCGGGCTCAGACTACCCCGCAATTGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGaa < 2:168813/150‑1 (MQ=255) gccaAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGaa < 1:676828/150‑1 (MQ=255) gccaAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGaa > 2:53139/1‑150 (MQ=255) ccaAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAg > 1:689120/1‑150 (MQ=255) caAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGt < 1:233329/150‑1 (MQ=255) aGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCAGCAGGAAGtgc < 1:31201/150‑2 (MQ=255) cgcAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAg > 2:554666/1‑150 (MQ=255) aaGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGcc < 2:545375/150‑1 (MQ=255) gCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATg > 2:661258/1‑150 (MQ=255) aCTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGAc > 1:315899/1‑150 (MQ=255) tGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTg > 1:461480/1‑150 (MQ=255) gggCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGc < 1:661258/150‑1 (MQ=255) ttACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCTTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGaa > 1:137851/1‑150 (MQ=255) tACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGaaa < 1:475265/150‑1 (MQ=255) tACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGaaa < 1:53139/150‑1 (MQ=255) tACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGaaa < 2:461480/150‑1 (MQ=255) ccTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAgg < 1:82579/150‑1 (MQ=255) cTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTCCACTTCCAGCAGGAAGtgcac > 1:111155/1‑108 (MQ=255) cTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTCCACTTCCAGCAGGAAGtgcac < 2:111155/108‑1 (MQ=255) aaCCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAg < 1:162645/150‑1 (MQ=255) cTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTc > 2:419630/1‑150 (MQ=255) cccAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTgg > 1:491592/1‑90 (MQ=255) cccAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTgg < 2:491592/90‑1 (MQ=255) gACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCtt < 1:556545/150‑1 (MQ=255) tACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTtgct > 2:752246/1‑150 (MQ=255) ccccGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGACCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGACGAGGTCAAGACCTtgctgc > 1:283934/1‑150 (MQ=255) ccGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCAt > 2:535598/1‑113 (MQ=255) ccGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCAt < 1:535598/113‑1 (MQ=255) aaTGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAag > 1:615621/1‑150 (MQ=255) tcTGCGCACCCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAgcg < 2:528537/149‑1 (MQ=255) aaCCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGGGCGTCCGc > 1:365831/1‑150 (MQ=255) cTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCa < 1:365662/82‑1 (MQ=255) cTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCa > 2:365662/1‑82 (MQ=255) gCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAgcctgc < 2:615621/150‑1 (MQ=255) ctcGATGAAACGGGGCCTGGACCTGTCCGGTGGTGTGCATTTCGTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAgcctgcc < 1:768494/148‑1 (MQ=255) ccGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAgcctgcct < 1:752246/150‑1 (MQ=255) gATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTAACGCAGCCTGCCTca > 2:441978/1‑150 (MQ=255) aaCTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACATTGCTGCGCAAAGATCGCGTCTGCTACGGCAGCCTGCCGCGGCAgg > 2:569421/1‑150 (MQ=255) gggCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAg < 2:22964/41‑1 (MQ=255) gggCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAg > 1:22964/1‑41 (MQ=255) ggCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATggc < 1:659450/150‑1 (MQ=255) ggCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATggc > 2:622737/1‑150 (MQ=255) ggACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGcat < 2:137851/150‑1 (MQ=255) cTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGAt > 2:410434/1‑138 (MQ=255) cTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGAt < 1:410434/138‑1 (MQ=255) tCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCATGATGCCGCCATCctgctg < 1:321923/150‑1 (MQ=255) tCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTg < 1:433997/150‑1 (MQ=255) tgCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTg > 2:575079/1‑150 (MQ=255) gCATTTCTTGCTGGGAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGCCCTTGCTGCGCAAAGAGCGCGTCCGCTCCCGCAGCCTGCATCAGCAGGATGGCGGAATCATGCTGGGCTTCGCTGa < 2:365831/150‑1 (MQ=255) cACTTCCAGCAGGAAGTGGACATGGACGAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGAc > 2:96915/1‑150 (MQ=255) ttCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGCATGCATCATGCTGGGCTTCGCTGACGAt < 1:419630/150‑1 (MQ=255) tgctgGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCt > 2:685000/1‑140 (MQ=255) tgctgGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCt < 1:685000/140‑1 (MQ=255) | CAGCCAAGGATGTAGTGCGCAAGGCACTGGGCGATGATTACGTCGTGGCCCTGAACCTGGCCCAGACTACCCCGCAATGGCTGCGCAACCTGGGCGCAAGCCCGATGAAACTGGGCCTGGACCTGTCCGGTGGTGTGCACTTCCTGCTGGAAGTGGACATGGACAAGGCCATGACTGCCCGCATGAAAGTCTACGAAGGCGAGGTCAAGACCTTGCTGCGCAAAGAGCGCGTCCGCTACCGCAGCCTGCCTCAGCAGGATGGCGGCATCATGCTGGGCTTCGCTGACGAT > NC_002947/971974‑972263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |