| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,031,395 | G→C | 19.2% | S33T (AGC→ACC) | regA → | photosynthetic apparatus regulatory protein RegA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,031,395 | 0 | G | C | 19.2% | 44.2 / 4.9 | 22 | S33T (AGC→ACC) | regA | photosynthetic apparatus regulatory protein RegA |
| Reads supporting (aligned to +/- strand): ref base G (9/8); new base C (2/2); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
GCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGCCCAAGCTGCTGGAACT > NC_002947/1031249‑1031539 | gCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAACCg > 1:156209/1‑150 (MQ=255) cACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGCTCATGGCCCGGGCCATGACCCgg < 1:141936/150‑2 (MQ=255) cGAACTGCGCCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTc > 2:622663/1‑150 (MQ=255) tcctcgcTTGCCATAGGGCAGGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCgg > 2:263579/5‑149 (MQ=255) aCTGCGCCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCgt < 1:622663/150‑1 (MQ=255) gcCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAg > 2:6405/1‑150 (MQ=255) gcCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGCTCATGGCCCGGGCCATGACCCgg < 2:119017/133‑2 (MQ=255) gcCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGCTCATGGCCCGGGCCATGACCCgg > 1:119017/1‑132 (MQ=255) cTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGcc > 1:531678/1‑150 (MQ=255) tGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGc < 2:531678/150‑1 (MQ=255) cACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCTCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGAc > 2:162600/1‑150 (MQ=255) gtggATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGTCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAgatggat < 1:6405/150‑1 (MQ=255) gATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTg < 1:299958/126‑1 (MQ=255) gATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTg > 2:299958/1‑126 (MQ=255) ttCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGAc > 1:592191/1‑138 (MQ=255) ttCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGAc < 2:592191/138‑1 (MQ=255) aCCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGtgctg > 2:388347/1‑150 (MQ=255) gacggctCATGGCCCGGGCCATGACCCGTCGCGGTTTCCGTGCGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGtgctgc > 2:763567/7‑150 (MQ=255) gggTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGAc < 1:739117/113‑1 (MQ=255) gggTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGAc > 2:739117/1‑113 (MQ=255) ggcggggCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATCCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCCGCTCCGGCCTGGGGCTGCTGCCCAAgctg < 2:156209/146‑1 (MQ=255) cATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGCCCAAGCTGCTGGAACt < 1:172290/150‑1 (MQ=255) | GCACCCTGACCGAACTGCGCCTGCCCTATGGCAAGCGAGGAGATGAATGATGAGCGAAGAAAACCAGGTCGAAAGCGAAGAGCTGCCGCACCTGCTGCTGGTGGATGACGATGCCACCTTCACCCGGGTCATGGCCCGGGCCATGAGCCGTCGCGGTTTCCGTGTGAGCACTGCCAGTTCTGCCGAAGAAGGCTTGATTCTGGCCCAGCAGGACCTGCCGGACTACGCCACGCTGGACCTGAAGATGGATGGCGACTCCGGCCTGGTGCTGCTGCCCAAGCTGCTGGAACT > NC_002947/1031249‑1031539 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |