Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,041,942 A→C 10.5% E141A (GAG→GCG)  colR → two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,041,9420AC10.5% 90.2 / 4.0 38E141A (GAG→GCG) colRtwo‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base A (16/18);  new base C (2/2);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

TTCCGCTCGGGGGCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACAGC  >  NC_002947/1041794‑1042087
                                                                                                                                                    |                                                                                                                                                 
ttCCGCTCGGGGGCTGACGACTACCTGCTCAAGACATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAg                                                                                                                                                  <  2:654760/150‑1 (MQ=255)
     cTCGGGGGCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTAc                                                                                                                                             <  1:519984/150‑1 (MQ=255)
             cTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGCGGGTAACCTCCAgg                                                                                                                                     <  2:554108/150‑1 (MQ=255)
              tGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAg                                                                                                                                                  <  2:791322/136‑1 (MQ=255)
              tGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAg                                                                                                                                                  >  1:791322/1‑136 (MQ=255)
                acgacTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGCGGGTAACCTCCAGGGta                                                                                                                                  <  1:640318/150‑2 (MQ=255)
                          gCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGGGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGCAGGTTACCCGCCAGGGTCGCCTGTTGaa                                                                                                                        <  2:303279/150‑1 (MQ=255)
                              aaGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGa                                                                                                                                                   >  2:509234/1‑119 (MQ=255)
                              aaGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGa                                                                                                                                                   <  1:509234/119‑1 (MQ=255)
                               aGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGCGGGTAACCTCCAgg                                                                                                                                     >  1:225102/1‑132 (MQ=255)
                                gCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCaa                                                                                                                  >  1:397967/1‑150 (MQ=255)
                                    tttGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCg                                                                                                              >  2:638132/1‑150 (MQ=255)
                                        cccTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGtt                                                                                                                           >  2:263582/1‑133 (MQ=255)
                                        cccTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGtt                                                                                                                           <  1:263582/133‑1 (MQ=255)
                                                 aGCTGGCGTCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCATGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAgc                                                                                                 <  2:258685/150‑1 (MQ=255)
                                                  gCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAgct                                                                                                <  1:471917/150‑1 (MQ=255)
                                                     ggcggcGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTCACCTCCGATCCCACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAgctgct                                                                                             <  1:495354/150‑1 (MQ=255)
                                                       cggcgCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTgg                                                                                           <  2:397967/150‑1 (MQ=255)
                                                          cgcgcATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCgg                                                                                        <  1:638132/150‑1 (MQ=255)
                                                               aTCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTg                                                                                   >  1:31240/1‑150 (MQ=255)
                                                                     tgcagggtgcGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACACTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTCATGATGCAg                                                                             >  1:11059/8‑150 (MQ=255)
                                                                        gTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGc                                                                               <  1:571126/145‑1 (MQ=255)
                                                                        gTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGACGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGc                                                                               >  2:571126/1‑145 (MQ=255)
                                                                         tgctgcGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTAATGATGCAGaaaa                                                                         >  1:286243/1‑150 (MQ=255)
                                                                          ggtgcGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAg                                                                                                                       <  1:112351/101‑1 (MQ=255)
                                                                          ggtgcGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAg                                                                                                                       >  2:112351/3‑103 (MQ=255)
                                                                                               tGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGa                                                   <  2:31240/150‑1 (MQ=255)
                                                                                                             tGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGGTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGaagaag                                     >  2:571229/1‑150 (MQ=255)
                                                                                                                    ggCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTtgtg                              <  2:216131/150‑1 (MQ=255)
                                                                                                                     gCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTgg                             >  2:323146/1‑150 (MQ=255)
                                                                                                                     gCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTgg                             >  2:587307/1‑150 (MQ=255)
                                                                                                                      cTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTggg                            <  1:587307/150‑1 (MQ=255)
                                                                                                                            tGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGaa                                                                                                                        <  2:549247/52‑1 (MQ=255)
                                                                                                                            tGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGaa                                                                                                                        >  1:549247/1‑52 (MQ=255)
                                                                                                                               gCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGGCGACt                   >  1:91228/1‑150 (MQ=255)
                                                                                                                                    gACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCt              <  2:724819/150‑1 (MQ=255)
                                                                                                                                      ccTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAGGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGgaagaa                                      <  2:524442/124‑1 (MQ=255)
                                                                                                                                      ccTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGgaagaa                                      >  1:524442/1‑124 (MQ=255)
                                                                                                                                            gaCCCTGGCGGGTAACCTCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGa      >  1:244789/2‑150 (MQ=255)
                                                                                                                                                cTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACAGc  >  1:581740/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TTCCGCTCGGGGGCTGACGACTACCTGCTCAAGCCATTTGCCCTGTCGGAGCTGGCGGCGCGCATCGAAGCGGTGCTGCGCCGTGCCCAGGGCGGTGGGCGGCGCACCCTGCAGGTGGCTGACCTGAGCTACGACCTCGATACCCTGGAGGTTACCCGCCAGGGTCGCCTGTTGAAGCTCAACCCGGTCGGCCTCAAGCTGCTGGCGGTACTGATGCAGAAAAGCCCGCACGTACTGCGCCGCGAGGTGCTGGAAGAAGCCTTGTGGGGCGACGACTGCCCTGATAGCGACAGC  >  NC_002947/1041794‑1042087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑