Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,164,645 T→G 55.9% T256P (ACC→CCC)  hexR ← DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,164,6450TG55.9% 18.2 / 47.2 43T256P (ACC→CCC) hexRDNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (8/11);  new base G (12/12);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01

GAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTGGCCAGTGGCGAGCCTGCGGCGGTGAGGCCCAGC  >  NC_002947/1164497‑1164793
                                                                                                                                                    |                                                                                                                                                    
gAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTc                                                                                                                                                     <  2:456375/150‑1 (MQ=255)
      ggCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCg                                                                                                                                               <  1:515452/150‑1 (MQ=255)
                       cgcACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAg                                                                                                                              <  1:719240/150‑1 (MQ=255)
                           ccTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGAGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACg                                                                                                                          >  2:79789/1‑150 (MQ=255)
                            cTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACgg                                                                                                                         >  2:525246/1‑150 (MQ=255)
                                        gcgcACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTg                                                                                                                                            <  1:147812/119‑1 (MQ=255)
                                        gcgcACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTg                                                                                                                                            >  2:147812/1‑119 (MQ=255)
                                               ggCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGa                                                                                                      <  2:80543/150‑1 (MQ=255)
                                                gCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAg                                                                                                                              <  2:292382/125‑1 (MQ=255)
                                                gCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAg                                                                                                                              >  1:292382/1‑125 (MQ=255)
                                                             cgtcgtcCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGt                                                                                        >  2:260362/1‑150 (MQ=255)
                                                               tcgtcCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGga                                                                                                                <  1:118710/124‑1 (MQ=255)
                                                               tcgtcCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGga                                                                                                                >  2:118710/1‑124 (MQ=255)
                                                                cgtcCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGa                                                                                     <  1:260362/150‑1 (MQ=255)
                                                                    cTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTc                                                                                 >  2:671834/1‑150 (MQ=255)
                                                                     tCGATCGGGTAACGGGTGGAGTTGAGGCTTTTCTGGATATTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCg                                                                                <  2:304204/150‑1 (MQ=255)
                                                                             gTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACg                                                                                                                          >  1:197918/1‑100 (MQ=255)
                                                                             gTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACg                                                                                                                          <  2:197918/100‑1 (MQ=255)
                                                                             gTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCg                                                                                >  2:400231/1‑142 (MQ=255)
                                                                             gTAACGGCTGGCGTTGAGGATTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCg                                                                                <  1:400231/142‑1 (MQ=255)
                                                                                     tGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCCTCCGGCAGCg                                                                <  1:503541/150‑1 (MQ=255)
                                                                                         gTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAAt                                                            <  1:525246/150‑1 (MQ=255)
                                                                                          ttGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAAtg                                                           >  1:163416/1‑150 (MQ=255)
                                                                                           tGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAAtgt                                                          <  2:163416/150‑1 (MQ=255)
                                                                                               gCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCCTGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGcag                                                      >  1:248423/1‑150 (MQ=255)
                                                                                                 tttCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGAAGCGGAATGTGcaggc                                                    <  1:671834/150‑1 (MQ=255)
                                                                                                     cTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGt                                                                                                                        >  1:796323/1‑78 (MQ=255)
                                                                                                     cTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGt                                                                                                                        <  2:796323/78‑1 (MQ=255)
                                                                                                     cTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGt                                                                                        >  2:707496/1‑110 (MQ=255)
                                                                                                     cTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGt                                                                                        <  1:707496/110‑1 (MQ=255)
                                                                                                     cTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTc                                                                                                  <  2:83030/100‑1 (MQ=255)
                                                                                                     cTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTc                                                                                                  >  1:83030/1‑100 (MQ=255)
                                                                                                        gATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGcaggctcaggct                                             >  1:664972/1‑150 (MQ=255)
                                                                                                            ttGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAg                                         >  2:53684/1‑150 (MQ=255)
                                                                                                            ttGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAg                                         <  1:79789/150‑1 (MQ=255)
                                                                                                            ttGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAg                                         <  1:53684/150‑1 (MQ=255)
                                                                                                                 cAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGGCATGCCGGTCGCCTGTCCCCCCAGCACTGTCCACTCTATCATTGCCTACGTTTCTGGTATGTAGTTGTTGGTATCTTCCGTCATCGGAATGTGAAGGATAAGGCTGCAGGCAtt                                    <  2:488707/150‑1 (MQ=255)
                                                                                                                                   cACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCa                                          <  2:495002/126‑1 (MQ=255)
                                                                                                                                   cACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCa                                          >  1:495002/1‑126 (MQ=255)
                                                                                                                                    aCGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTGGCCAGTGGCGAGCCTgcg                 >  2:494131/1‑150 (MQ=255)
                                                                                                                                    aCGCCGCGGCGCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTGGCCAGTGGCGAGCCTgcg                 >  2:549028/1‑150 (MQ=255)
                                                                                                                                         gcggcgCAGGGGCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATATGTAGGCTCGCGCAGCATGCATTCGCCAGTGGCGACGCAGcggcggc            >  2:131011/1‑149 (MQ=255)
                                                                                                                                                   gTCATGCCGGTGGCCCGTACATAGCGCACGGTCAGCTGGATTATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTGGCCAGTGGCGAGCCTGCGGCGGTGAGGCCCAGc  <  2:248423/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAGCCTGGCACCTGGGCAGCGTGCGCACCTGCGTTTGCAGGCGCACCGGCTCAGTTGAGGTCGTCGTCCTCGATCGGGTAACGGCTGGCGTTGAGGCTTTCCTTGATCTTGCGCAGGTGAGGCTGGAAGTCCACGCCGCGGCGCAGGGTCATGCCGGTGGCCAGTACATCGAGCACGGTCAGCTGGATGATCCGCGAGGTCATTGGCATGTAGATGTCGGTATCTTCCGGCAGCGGAATGTGCAGGCTCAGGCTGCAGGCATTGGCCAGTGGCGAGCCTGCGGCGGTGAGGCCCAGC  >  NC_002947/1164497‑1164793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑