Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,212,017 C→T 8.2% Q152Q (CAG→CAA)  PP_1060 ← glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,212,0170CT 8.2% 128.5 / 3.3 49Q152Q (CAG→CAA) PP_1060glutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (20/25);  new base T (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

TAGGGGCTGATGCTGCCCTCGCCGGTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGCCGATAAACTCGAAACCGGATTCATATACGTCGATCAGGGTGCCGAAAGGTTTGTCCGA  >  NC_002947/1211880‑1212151
                                                                                                                                         |                                                                                                                                      
tAGGGGCTGATGCTGCCCTCGCCGGTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGc                                                                                                                            >  2:338258/1‑150 (MQ=255)
  ggggCTGATGCTGCCCTCGCCGGTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCgg                                                                                                                          <  1:249430/150‑1 (MQ=255)
     gCTGATGCTGCCCTCGCCGGTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCAGTGCGGcca                                                                                                                       <  2:678007/150‑2 (MQ=255)
     gCTGATGCTGCCCTCGCCGGTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGccc                                                                                                                       <  2:256960/150‑1 (MQ=255)
             tgcCCTCGCCGGTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGATCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACcgacga                                                                                                               >  1:369578/1‑150 (MQ=255)
             tgcCCTCGCCGGTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGTCCCACcgacga                                                                                                               <  2:273028/150‑1 (MQ=255)
                ccTCGCCGGTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTgg                                                                                                                                     <  2:31561/125‑1 (MQ=255)
                ccTCGCCGGTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTgg                                                                                                                                     >  1:31561/1‑125 (MQ=255)
                ccTCGCCGGTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGa                                                                                                            >  1:698885/1‑150 (MQ=255)
                ccTCGCCGGTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGa                                                                                                            >  2:665399/1‑150 (MQ=255)
                 cTCGCCGGTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGac                                                                                                           >  2:534792/1‑150 (MQ=255)
                       ggTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGaa                                                                                                     <  1:135747/150‑1 (MQ=255)
                        gtgtCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAg                                                                                                    <  2:698885/150‑1 (MQ=255)
                                gtggAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCt                                                                                                                                       <  2:157080/107‑1 (MQ=255)
                                gtggAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCt                                                                                                                                       >  1:157080/1‑107 (MQ=255)
                                gtggAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGc                                                                                                   <  1:246859/143‑1 (MQ=255)
                                gtggAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGc                                                                                                   >  2:246859/1‑143 (MQ=255)
                                           ccAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGTTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCg                                                                                 <  2:502578/150‑1 (MQ=255)
                                           ccAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGTTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCg                                                                                 >  1:502578/1‑150 (MQ=255)
                                           ccAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGTGGGCCGCAGTGCGGccagc                                                                                                                     >  2:18324/1‑111 (MQ=255)
                                           ccAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGTGGGCCGCAGTGCGGccagc                                                                                                                     <  1:18324/114‑4 (MQ=255)
                                           ccAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTcagccagc                                                                                  >  2:340646/1‑149 (MQ=255)
                                           ccAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTcagccagc                                                                                  <  1:340646/149‑1 (MQ=255)
                                           ccAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTcagcca                                                                                    >  1:555019/1‑147 (MQ=255)
                                           ccAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTcagcca                                                                                    <  2:555019/147‑1 (MQ=255)
                                           ccAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGAGGACACGGAAGCTGGTCGGGTCAGCCAGCg                                                                                 >  2:48882/1‑150 (MQ=255)
                                           ccAGCTTCGCGGCCTGGTTTAGCGCACGGCTGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCg                                                                                 >  2:231962/1‑150 (MQ=255)
                                                ttGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGcc                                                                            <  1:246821/150‑1 (MQ=255)
                                                     gcCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGaa                                                                                                     <  2:777141/120‑1 (MQ=255)
                                                     gcCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGaa                                                                                                     >  1:777141/1‑120 (MQ=255)
                                                         tGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATg                                                                   <  1:338258/150‑1 (MQ=255)
                                                                gcgcACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGc                                                            <  1:48882/150‑1 (MQ=255)
                                                                    aCGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGCCGAt                                                        <  1:466527/150‑1 (MQ=255)
                                                                      ggATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGCCGATaa                                                      >  1:284354/1‑150 (MQ=255)
                                                                          ggCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGCCGATAAACTc                                                  <  2:133229/150‑1 (MQ=255)
                                                                          ggCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGCCGATAAACTc                                                  <  1:441405/150‑1 (MQ=255)
                                                                                 gtgcTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGATGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGCCGATAAACTCGAAACCg                                           <  1:485368/148‑1 (MQ=255)
                                                                                 gcgcTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCANCGGTGCCGGGCGCATGGAGTGGCCGATAAACTCGAAACCg                                           <  1:534792/150‑1 (MQ=255)
                                                                                 gcgcTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGCCGATAAACTCGAAACCg                                           <  1:665399/150‑1 (MQ=255)
                                                                                             ccGAAACTCATGGCCGAAATGTGGAAGATCGACGCGGAATAGGGCTGGGCGCACTGCGGGGCACCGACGATGACACGGAAGCTGGTCGCGTCAGACAGCCGTCCGGTGCGTGTGGTTTGTCGGAGAAACTGTGAACCGGATGCATATACg                               >  2:48224/1‑150 (MQ=255)
                                                                                                aaaCTCATGGCCGAAATGTTGAAGATCGACGCGGAATACTCCTGGCCGCACTGCGGCCCACCGACGATGAGACGGAAGCTCGTCGGGTCAGCCAGCAGTGCCGGGCGCACGGAGTGGACGATAACATCGGAACCGGAAGCATCTACATCg                            >  2:137652/1‑150 (MQ=255)
                                                                                                    tCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGCCGATAAACTCGAAACCGGCTTCATATACGTGGacga                        >  1:58744/1‑147 (MQ=255)
                                                                                                      aTGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGCCGATAAACTCGAAACCGGATTCATATACGTCGATCAgg                      <  2:452539/150‑1 (MQ=255)
                                                                                                      aTGGCCGAAATGTTGAACATCGACGCTGAATACGGCTGGCCTCACTGCCGCCCACCGACTATGACACTGAAGCTGGGCCGCTCAGCCAGCGGTGCCGGGCGCATGTATTTGCCGATAAACGCGAAACCGGATTCATATACGTCGATCAgg                      <  2:58744/150‑1 (MQ=255)
                                                                                                                    gAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGCCGATAAACTCGAAACCGGATTCATATACGTCGATCAGGGTGCCGAAAGGttt        >  1:410848/1‑150 (MQ=255)
                                                                                                                       gATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTcagc                                                                                      >  2:257585/1‑69 (MQ=255)
                                                                                                                       gATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTcagc                                                                                      <  1:257585/69‑1 (MQ=255)
                                                                                                                                    tACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGCCGATAAACTCGAAACCGGATTCATATACGTCGATCAGGGTGCCGAAAGGTTTGTCCGa  <  2:121098/140‑1 (MQ=255)
                                                                                                                                    tACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGCCGATAAACTCGAAACCGGATTCATATACGTCGATCAGGGTGCCGAAAGGTTTGTCCGa  >  1:121098/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TAGGGGCTGATGCTGCCCTCGCCGGTGTCATGGTGGAAATTGCCCAGCTTGGCGCCCTGGTTGAGCGCACGGATGGCATTGGCGCTGAGCGAGCCGAAACTCATGGCCGAAATGTTGAAGATCGACGCGGAATACGGCTGGCCGCACTGCGGCCCACCGACGATGACACGGAAGCTGGTCGGGTCAGCCAGCGGTGCCGGGCGCATGGAGTGGCCGATAAACTCGAAACCGGATTCATATACGTCGATCAGGGTGCCGAAAGGTTTGTCCGA  >  NC_002947/1211880‑1212151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑