Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,221,043 T→G 11.9% D90A (GAC→GCC)  dctD‑II ← C4‑dicarboxylate transport transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,221,0430TG11.9% 93.2 / 8.5 42D90A (GAC→GCC) dctD‑IIC4‑dicarboxylate transport transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (18/19);  new base G (3/2);  total (21/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.83e-01

CGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCA  >  NC_002947/1220897‑1221187
                                                                                                                                                  |                                                                                                                                                
cGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGa                                                                                                                                               >  2:238186/1‑150 (MQ=255)
cGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGa                                                                                                                                               <  2:32189/150‑1 (MQ=255)
               gcAGGGCCAACACTTCGCATGACATTCGACGCATTGCCAGCCCGCGGCGTACCAAGTCGGTCTGCTTTGCAGCGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAg                                                                                                                                <  1:216227/150‑1 (MQ=255)
                     ccaccaCTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCa                                                                                                                          >  2:344437/1‑150 (MQ=255)
                            ttCGCGTGACAGCCCGCGCTGTTCCAGCGCGCTGCGTCCCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCa                                                                                                                          >  1:514309/1‑143 (MQ=255)
                            ttCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTGCCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCa                                                                                                                          <  2:514309/143‑1 (MQ=255)
                                   gACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAgg                                                                                                            >  2:494094/1‑150 (MQ=255)
                                         ccGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCgg                                                                                                      >  2:470663/1‑150 (MQ=255)
                                         ccGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCgg                                                                                                      >  1:45600/1‑150 (MQ=255)
                                              cTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGGGGTCACg                                                                                                 >  2:638458/1‑150 (MQ=255)
                                                       cgcgcgGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAg                                                                                        <  1:238186/150‑1 (MQ=255)
                                                           cggcgTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGa                                                                                    <  2:45600/150‑1 (MQ=255)
                                                                  ccACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGagca                                                                             <  2:173589/150‑1 (MQ=255)
                                                                  ccACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGAGCCAACGGCCATGTCGATATCGCCATAGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGagca                                                                             >  1:241314/1‑150 (MQ=255)
                                                                  ccACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCAGGAAGTCGTAGGCGCCCTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGagca                                                                             <  1:330363/150‑1 (MQ=255)
                                                                   cACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGa                                                                                    <  2:738102/142‑1 (MQ=255)
                                                                   cACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGa                                                                                    >  1:738102/1‑142 (MQ=255)
                                                                    aCGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGGCGATATCGACATGGc                                                                                                                                   <  2:397251/94‑1 (MQ=255)
                                                                    aCGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGGCGATATCGACATGGc                                                                                                                                   >  1:397251/1‑94 (MQ=255)
                                                                           ccAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGc                                                                    <  1:344437/150‑1 (MQ=255)
                                                                            cAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGcc                                                                                                                                        <  1:366997/81‑1 (MQ=255)
                                                                            cAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGcc                                                                                                                                        >  2:366997/1‑81 (MQ=255)
                                                                            cAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGcc                                                                   >  1:660586/1‑150 (MQ=255)
                                                                              ggCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCAt                                                                 <  1:679502/150‑1 (MQ=255)
                                                                                  cTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAAt                                                             <  2:660586/150‑1 (MQ=255)
                                                                                      ggggAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATAccc                                                         >  2:189497/1‑150 (MQ=255)
                                                                                            aaCGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAgg                                                   >  1:246223/1‑150 (MQ=255)
                                                                                                           aaGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTgacg                                    >  1:597489/1‑150 (MQ=255)
                                                                                                                    gcgcCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCCCCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCg                           <  1:494094/150‑1 (MQ=255)
                                                                                                                    gcgcCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCg                           <  1:470663/150‑1 (MQ=255)
                                                                                                                      gcCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGagc                                                                              <  1:515986/97‑1 (MQ=255)
                                                                                                                      gcCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGagc                                                                              >  2:515986/1‑97 (MQ=255)
                                                                                                                              gCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCa                                                               >  2:339558/1‑104 (MQ=255)
                                                                                                                              gCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCa                                                               <  1:339558/104‑1 (MQ=255)
                                                                                                                                aTGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCa                                                                       <  1:58786/94‑1 (MQ=255)
                                                                                                                                aTGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCa                                                                       >  2:58786/1‑94 (MQ=255)
                                                                                                                                     gcCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGAt          <  2:597489/150‑1 (MQ=255)
                                                                                                                                          tggCCATGGCGATATCGACATGGCCGGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGAtt                                                                                  <  1:242348/72‑1 (MQ=37)
                                                                                                                                          tggCCATGGCGATATCGACATGGCCGGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGAtt                                                                                  >  2:242348/2‑73 (MQ=37)
                                                                                                                                             ccATGGCGATATCGACATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCa  >  1:503771/1‑150 (MQ=255)
                                                                                                                                               aTGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTga                                      >  2:768060/1‑112 (MQ=255)
                                                                                                                                               aTGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTga                                      <  1:768060/112‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CGGCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCA  >  NC_002947/1220897‑1221187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑