| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,235,338 | T→C | 15.7% | intergenic (‑47/+5) | glpK ← / ← glpF | glycerol kinase/aquaglyceroporin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,235,338 | 0 | T | C | 15.7% | 70.4 / 4.8 | 31 | intergenic (‑47/+5) | glpK/glpF | glycerol kinase/aquaglyceroporin |
| Reads supporting (aligned to +/- strand): ref base T (9/17); new base C (2/3); total (11/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGTCACGGTCGAAAATGATCGCCCGCGAACTGGTGGTACCCTGGTCCAGGGCGATGATGTAGTTCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCAGGCCGCGATAGCTCGCTGCACCCAAGCAGGCGCCAAGGATCGGTGCAAATAC > NC_002947/1235206‑1235477 | gCGTCACGGTCGAAAATGATCGCCCGCGAACTGGTGGTACCCTGGTCCAGGGCGATGATGTAGTTCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGa < 1:743236/150‑1 (MQ=255) cGAAAATGATCGCCCGCGAACTGGTGGTACCCTGGTCCAGGGCGATGATGTAGTTCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATTGTGGCATCAATTGGCTTGAGTATCGCCCt < 2:141227/150‑1 (MQ=255) cgcgAACTGGTGGTACCCTGGTCCAGGGCGATGATGTAGTTCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCAttt < 1:731694/150‑1 (MQ=255) gAACTGGTGGTACCCTGGTCCAGGGCGATGATGTAGTTCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGtc > 2:724964/1‑150 (MQ=255) gtggtACCCTGGTCCAGGGCGATGATGTAGTTCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATAAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAggg < 1:273362/150‑1 (MQ=255) ccTGGTCCAGGGCGATGATGTAGTTCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATc < 1:533020/115‑1 (MQ=255) ccTGGTCCAGGGCGATGATGTAGTTCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAAGTGGCTTGAGTATc > 2:533020/1‑115 (MQ=255) tCCAGGGCGATGATGTAGTTCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCggcgggc < 1:370966/150‑1 (MQ=255) tCCAGGGCGATGATGTAGTTCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTAGCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCggcgggc < 1:414296/150‑1 (MQ=255) gggCGATGATGTAGTTCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTTATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCa > 1:573001/1‑150 (MQ=255) gTTCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCg < 1:369357/150‑1 (MQ=255) ttCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGTGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGt < 1:86442/150‑1 (MQ=255) cTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGGGc > 1:363788/1‑150 (MQ=255) tctctcTTGGTGGTAGGTCTTTCCAGTATACTGTGTCGGGGCTGTGCGTGATGGCGGCATCATTTGGCGTGGGTATCGCCCTGGCAAATGTCATGGGTCTCGGGTGTGGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCAGGCCGCGa < 2:45954/150‑1 (MQ=255) tcATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGc > 2:575531/1‑81 (MQ=255) tcATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGc < 1:575531/81‑1 (MQ=255) tcATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCAGGCCGCGATAGc > 2:499631/1‑150 (MQ=255) aTGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCa < 2:285417/136‑1 (MQ=255) aTGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCa > 1:285417/1‑136 (MQ=255) tGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCAgg < 1:124727/137‑1 (MQ=255) tGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCAgg > 2:124727/1‑137 (MQ=255) tGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCAGGCCGCGa < 2:398377/143‑1 (MQ=255) tGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCAGGCCGCGa > 1:398377/1‑143 (MQ=255) tGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCAGGCCGCGATAGCTCg < 1:611357/150‑1 (MQ=255) cTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCAGGCCGCGATAGCTCGCTGCACCCAAg < 1:499631/150‑1 (MQ=255) cTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGCGGCATCAATTGGCTTGAGTa < 2:554908/57‑1 (MQ=255) cTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGCGGCACCAATTGGCTTGAGTa > 1:554908/1‑57 (MQ=255) cTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGCGGCACCAATTGGCTTGAGTa > 1:554894/1‑57 (MQ=255) cTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGCGGCACCAATTGGCTTGAGTa < 2:554894/57‑1 (MQ=255) cAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCAGGCCGCGATAGCTCGCTGCACCCAAGCAgg < 1:791605/150‑1 (MQ=255) aGGGGGGCAGGTCTGTGCGTGTTGGTGGCATCGATTGGCTTTAGGATCGCCCTGGCGAAGGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTGGCGTGGAATCAGGCCGCGATAGCTCGCTGCACCCAAGCAGGCGCCAAg < 2:363788/150‑1 (MQ=255) tggCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCAGGCCGCGATAGCTCGCTGCACCCAAGCAGGCGCCAAGGATCGGTGCAAATAc > 1:31494/1‑140 (MQ=255) tggCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCAGGCCGCGATAGCTCGCTGCACCCAAGCAGGCGCCAAGGATCGGTGCAAATAc < 2:31494/140‑1 (MQ=255) | GCGTCACGGTCGAAAATGATCGCCCGCGAACTGGTGGTACCCTGGTCCAGGGCGATGATGTAGTTCTTATCCTGGGTGTCTGTCATGGTCGTAGGCCTTGCAAGAATAGGGGGGCAGGGCTGTGCGTGATGGTGGCATCAATTGGCTTGAGTATCGCCCTGGCGAATGTCATTTGTCTCAGGGGTCGCGGCGGGCGCCATTGGCAGGTTGCGTGCAATCAGGCCGCGATAGCTCGCTGCACCCAAGCAGGCGCCAAGGATCGGTGCAAATAC > NC_002947/1235206‑1235477 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |