Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,291,468	A→C	7.9%	K85Q (AAG→CAG)	estC →	carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,291,468	0	A	C	7.9%	123.6 / 2.4	46	K85Q (AAG→CAG)	estC	carboxylesterase
Reads supporting (aligned to +/- strand):  ref base A (21/21);  new base C (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGGTGGCGAAACCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGTATGACTGG  >  NC_002947/1291321‑1291617                                                                                                                                                    |                                                                                                                                                      gtgggtggCGAAACCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAg                                                                                                                                                     >  2:785065/1‑150 (MQ=255)         cGAAACCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCCAGCtttccct                                                                                                                                             >  2:795984/1‑144 (MQ=255)            aaCCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCCAGCTTTCCCTCgc                                                                                                                                          <  1:795984/150‑2 (MQ=255)            aaCCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCAAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGa                                                                                                                                          >  1:137119/1‑150 (MQ=255)                gtcgtTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGcc                                                                                                                                      >  2:441443/1‑150 (MQ=255)                      gACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTg                                                                                                                                <  1:785065/150‑1 (MQ=255)                      gACCGGTGGGCCGGCAGGGGCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGAGAGGGCAAGCTGGCCCTCGATGCCCCGGTg                                                                                                                                <  2:194560/150‑1 (MQ=255)                        ccTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGc                                                                                                                              <  1:729174/150‑1 (MQ=255)                               gCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAactac                                                                                                                       <  1:186508/150‑1 (MQ=255)                                    cAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGcc                                                                                                                  <  1:378561/150‑1 (MQ=255)                                     aGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGccc                                                                                                                 >  1:534603/1‑150 (MQ=255)                                       tGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGa                                                                                                               >  2:670598/1‑150 (MQ=255)                                        gCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTTGCCAACTACTGGCCCGAg                                                                                                              <  2:23040/150‑1 (MQ=255)                                           gACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTc                                                                                                           <  1:670598/150‑1 (MQ=255)                                            aCAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCg                                                                                                          >  1:765497/1‑150 (MQ=255)                                              aaGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGcc                                                                                                        <  1:441443/150‑1 (MQ=255)                                                         gccggCCTGGCACAGCCATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTaa                                                                                             <  2:170854/147‑1 (MQ=255)                                                              ccTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGCTCGCCCAGGCGGGTAAGCAGt                                                                                        >  2:263375/1‑150 (MQ=255)                                                               cTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTc                                                                                       >  2:616617/1‑150 (MQ=255)                                                                    acaGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCgg                                                                                                 >  1:204713/1‑135 (MQ=255)                                                                    acaGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCgg                                                                                                 <  2:204713/135‑1 (MQ=255)                                                                    acaGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGAGATAGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCa                                                                                  >  1:618847/1‑150 (MQ=255)                                                                             ccATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTc                                                                         >  1:157043/1‑150 (MQ=255)                                                                              cATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCa                                                                        >  2:116719/1‑150 (MQ=255)                                                                                   ccAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGTCGGGTAAGCAGTCGATCACCTTGCGTCAgctgc                                                                   >  2:235637/1‑150 (MQ=255)                                                                                      aCCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCa                                                                <  2:746602/150‑1 (MQ=255)                                                                                        cTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGc                                                              <  2:157043/150‑1 (MQ=255)                                                                                                cTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTgg                                                                                                                    >  2:54057/1‑88 (MQ=255)                                                                                                cTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTgg                                                                                                                    <  1:54057/88‑1 (MQ=255)                                                                                                             ttCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGc                                          >  1:399056/1‑149 (MQ=255)                                                                                                             ttCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGc                                          <  2:399056/149‑1 (MQ=255)                                                                                                                    ccGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGCTCGCTAACCCACGTCATCAGGCCAGCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTg                                  >  1:258339/1‑150 (MQ=255)                                                                                                                     cGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGa                                 >  2:292468/1‑150 (MQ=255)                                                                                                                                tGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCgg                      >  1:102435/1‑150 (MQ=255)                                                                                                                                    gTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCCGCCATTCGTGAGCCGCTGCCGGCTGa                  >  2:657482/1‑150 (MQ=255)                                                                                                                                     ttGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGCTGGCCAACTACTGGCCCGAATTCGCCCAGGCGGGTAAGCATTCGACCACCTTTCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGaa                 <  1:263375/150‑1 (MQ=255)                                                                                                                                         tCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGcc              >  2:84698/1‑149 (MQ=255)                                                                                                                                         tCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGcc              <  1:84698/149‑1 (MQ=255)                                                                                                                                          cGGCGAGGGTCCGCTCTCGCTCTATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCtt            <  2:618847/150‑1 (MQ=255)                                                                                                                                          cGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCtt            <  2:534603/150‑1 (MQ=255)                                                                                                                                          cGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCtt            <  2:765497/150‑1 (MQ=255)                                                                                                                                          cGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCtt            <  1:116719/150‑1 (MQ=255)                                                                                                                                           atcGAGGGCCAGCTTGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCAGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTg           >  1:729484/3‑150 (MQ=255)                                                                                                                                             cGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGTa         <  1:674969/150‑1 (MQ=255)                                                                                                                                              gAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGTAt        <  2:102435/150‑1 (MQ=255)                                                                                                                                              gAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGTAt        >  1:113815/1‑150 (MQ=255)                                                                                                                                                    aaGCTGGCCCTCGATGCCCCGGTGGTCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGTATGACTgg  <  1:37572/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                      GTGGGTGGCGAAACCGTCGTTGACCTGTGGGCCGGCAGTGCCGACAAGGACGGCCAGCAGGCCTGGCACAGCGATACCATCGCCAACCTGTTCTCCTGCACCAAGACCTTCGCGGCCGTTACCGCCCTGCAGTTGGTCGGCGAGGGCAAGCTGGCCCTCGATGCCCCGGTGGCCAACTACTGGCCCGAGTTCGCCCAGGCGGGTAAGCAGTCGATCACCTTGCGTCAGCTGCTCAGCCACCGTGCCGGCTTGCCGGCCATTCGTGAGCTGCTGCCGGCTGAAGCCTTGTATGACTGG  >  NC_002947/1291321‑1291617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑