Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,376,328	G→C	21.0%	intergenic (+31/+18)	kup → / ← PP_1201	potassium transport system protein Kup/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,376,328	0	G	C	21.0%	38.7 / 5.2	19	intergenic (+31/+18)	kup/PP_1201	potassium transport system protein Kup/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (8/7);  new base C (2/2);  total (10/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTT‑CAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCA  >  NC_002947/1376190‑1376466                                                                                                                                            |                                                                                                                                           gggggCAACCTGTTCGCGTTCTTGCTGAAGAATGCCACAGACAACAAACGCTCCTTAAAACCCGCC‑TCCATCGGCCTGTCCGTGCTGTTGAGTCTGGTCCAGTTCTCAATGGGGTTATACATCAGGCTCAGGGCCGCTCTGCGGCCCTGa                                                                                                                                 <  2:488478/149‑1 (MQ=255) cGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTT‑CAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCTCTGCGGCCCTGa                                                                                                                                 <  2:294605/150‑1 (MQ=255)   gggCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTT‑CAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGc                                                                                                                               <  1:455791/150‑1 (MQ=255)                              gaaTGCCAACGGCAACTTGCGCTTCTT‑CAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTgg                                                                                                    >  1:388251/1‑150 (MQ=255)                                 tGCCAACGGCAACTTGCGCTTCTT‑CAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACt                                                                                                 >  1:391675/1‑150 (MQ=255)                                        ggCAACTTGCGCTTCTT‑CAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTg                                                                                          >  2:471696/1‑150 (MQ=255)                                         gCAACTTGCGCTTCTT‑CAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTgg                                                                                         <  1:33079/150‑1 (MQ=255)                                                 cgcTTCTT‑CAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTc                                                                                 <  2:388251/150‑1 (MQ=255)                                                                      gAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTg                                                                                          <  2:622995/121‑1 (MQ=255)                                                                      gAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTg                                                                                          >  1:622995/1‑121 (MQ=255)                                                                            ggTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAt                                                                              >  1:180025/1‑127 (MQ=255)                                                                            ggTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGAt                                                                              <  2:180025/127‑1 (MQ=255)                                                                                         gggACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCt                                                                                                                <  2:176470/80‑1 (MQ=255)                                                                                         gggACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCt                                                                                                                >  1:176470/1‑80 (MQ=255)                                                                                                aGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCtcgtcg                                   >  2:261778/1‑150 (MQ=255)                                                                                                                ggggTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAg                   <  1:471696/150‑1 (MQ=255)                                                                                                                                 tCAGGGCCGCTCTGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGgcagatc  >  1:175197/1‑145 (MQ=255)                                                                                                                                 tCAGGGCCGCTCTGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCa  >  2:408291/1‑150 (MQ=255)                                                                                                                                 tCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGATAGTTGTCGATGAAGTGGTGCCCGCCAGGCGGTTGCAGGCGCTCa  >  2:288404/1‑150 (MQ=255)                                                                                                                                            |                                                                                                                                           CGGGGCAACCTGTTCGCGTTCTTGCTGAAGAATGCCAACGGCAACTTGCGCTTCTT‑CAACCTGCCGTTGAACCGGGTGATCGAGCTAGGGACCCAGGTCGAGATCTGAATGGGGTTATACAGCAGGCTCAGGGCCGCAGAGCGGCCCTGAGCCTTGTCAGTTCTGCTCAGCTACGCTGGACTTGCCTTGGCGCGTTTCGATCTCGCCAATCAACCGCTTGGCGAGTGCCGGGTAGTTCTCGTCGAAGTGGTGCCCGCCAGGCAGTTTCAGGCGCTCA  >  NC_002947/1376190‑1376466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑