| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,388,050 | T→A | 13.3% | D149V (GAC→GTC) | PP_1209 ← | cold‑shock protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,388,050 | 0 | T | A | 13.3% | 115.8 / 10.0 | 46 | D149V (GAC→GTC) | PP_1209 | cold‑shock protein |
| Reads supporting (aligned to +/- strand): ref base T (21/18); new base A (3/3); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01 | |||||||||||
AACGGCGGTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGCAC > NC_002947/1387904‑1388189 | aaCGGCGGTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGtctc > 1:527141/1‑150 (MQ=255) ggcggTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCggg > 1:164077/1‑150 (MQ=255) ggcggTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCggg > 2:123789/1‑150 (MQ=255) ggTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCTGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGgaga < 1:269323/150‑1 (MQ=255) tGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAAcc > 2:118556/1‑150 (MQ=255) ccACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGaaa < 1:596017/143‑1 (MQ=255) caaCGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGaaa > 2:596017/3‑143 (MQ=255) cACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGcc > 1:82757/1‑150 (MQ=255) cGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCtt > 2:745855/1‑150 (MQ=255) cGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGAATTGACTGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGGCTCGGGAGGTGAAACCAAAGCCtt > 1:535817/1‑150 (MQ=255) gTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCttt < 1:348214/150‑1 (MQ=255) tCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCCGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTc < 1:572898/150‑1 (MQ=255) ttCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCTCCCGAGACTCGGGCGAt < 1:381232/132‑1 (MQ=255) ttCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCTCCCGAGACTCGGGCGAt > 2:381232/1‑132 (MQ=255) ttCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGa > 2:119874/1‑150 (MQ=255) cTGCAGGCCCTTGTCGCGGTGC‑CCACCGTAAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATTGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGtt > 1:706763/1‑150 (MQ=255) gCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCa < 2:164077/150‑1 (MQ=255) gTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTgcc < 2:610390/150‑3 (MQ=255) gCATCACCG‑AAAACTCCCCGCGCTGGCCTTCGACCAGAATGCGGTGGCCCT‑AGCGAGTTGTGGTCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCgg < 2:706763/150‑1 (MQ=255) aCCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCa < 1:123789/150‑1 (MQ=255) cG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCTCCCGAGACTCgggcga > 2:387937/1‑95 (MQ=255) cG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCTCCCGAGACTCGGgcga < 1:387937/98‑1 (MQ=255) tCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCa < 1:388998/141‑1 (MQ=255) tCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCa > 2:388998/1‑141 (MQ=255) tCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCg > 1:97597/1‑150 (MQ=255) tCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCg > 1:357149/1‑150 (MQ=255) gcTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCTCCCGAGACTCGGGCGAt > 1:726470/1‑85 (MQ=39) gcTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCTCCCGAGACTCGGGCGAt < 2:726470/85‑1 (MQ=39) tGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTtgt < 2:82757/150‑1 (MQ=255) tGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGAGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTtgt < 2:535817/150‑1 (MQ=255) ttCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCAc > 1:339087/1‑150 (MQ=255) ccAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGtt < 1:709716/150‑1 (MQ=255) aaTGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGt > 2:505636/1‑150 (MQ=255) cTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCtt < 1:505636/150‑1 (MQ=255) cg‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGtg > 1:505857/1‑150 (MQ=255) cg‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGtg < 2:505857/150‑1 (MQ=255) gATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGtgct < 1:118556/150‑1 (MQ=255) cccGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCttt < 2:32066/58‑1 (MQ=255) cccGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCttt > 1:32066/1‑58 (MQ=255) ccGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGc > 2:405444/1‑150 (MQ=255) aaaGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTgc < 1:244934/134‑1 (MQ=255) aaaGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTgc > 2:244934/1‑134 (MQ=255) aCGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCtt < 2:234647/97‑1 (MQ=255) aCGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCtt > 1:234647/1‑97 (MQ=255) gAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGc > 2:256277/1‑150 (MQ=255) aaGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGcc > 2:242437/1‑150 (MQ=255) aTCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGcac > 2:413851/1‑150 (MQ=255) | AACGGCGGTTTACTGCAACCACGTCTTCGGCCTGCAGGCCCTTGTCGCGGTGCATCACCG‑AAAACTCCACGCGCTGGCCTTCGACCAGAATGCGGTGGCCCTCACCGCG‑GATGGCCCGAAAGTGCACGAAGATGTCATCGCCCGAGTCTCGGGAGATGAAACCAAAGCCTTTCGACGTGTTGAACCATTTGACGGTGCCGGTATCACGGTTGCCGGCATCTTGTGCCACGTGTTGAGTGCCGCGTGCCTTGCGCGTGCTGCGGGCAAAGCCCACGGCCAGATGCAC > NC_002947/1387904‑1388189 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |