| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 146,128 | T→A | 13.7% | intergenic (+33/‑115) | gltP → / → PP_0138 | glutamate/aspartate‑proton DAACS transporter/membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 146,128 | 0 | T | A | 13.7% | 131.5 / 13.1 | 51 | intergenic (+33/‑115) | gltP/PP_0138 | glutamate/aspartate‑proton DAACS transporter/membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (23/21); new base A (4/3); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
TCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCGGCTTTTT > NC_002947/145983‑146271 | tCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCg > 2:516713/1‑150 (MQ=255) cATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCgg < 2:464848/150‑1 (MQ=255) cATTGCGCGGGGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCgg > 2:756182/1‑150 (MQ=255) ttGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAGACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCgggg < 2:494599/150‑1 (MQ=255) tCGGTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACAAGTCGGGGCtct < 1:46933/150‑3 (MQ=255) tCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCttt > 1:634965/1‑150 (MQ=255) tCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCttt < 2:332758/150‑1 (MQ=255) ggAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGTAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTa < 1:735508/150‑1 (MQ=255) gAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGTTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTCTTAt < 2:332859/150‑1 (MQ=255) gAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTAt < 1:516713/150‑1 (MQ=255) aggTACGCGGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCCAGAAGAAGGGAGCTGCAGTGGTTGGCGAAACCGCCAAGCGCTGGGCCTGATGGCTGTTTCACCAAGCCCCCCGACCTGTGGGGGCTTTTTGTTATCTGCga < 2:365672/148‑1 (MQ=255) acgCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTg > 2:13270/1‑150 (MQ=255) ggTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGcc < 1:13270/150‑1 (MQ=255) gTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCgggg < 1:459934/121‑1 (MQ=255) gTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCgggg > 2:459934/1‑121 (MQ=255) cAAGGGCGAGCAGTACTACGCCTCTCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAAAGCCCCGACAAGTCGGGGCtctt > 2:257682/1‑121 (MQ=255) cAAGGGCGAGCAGTACTACGCCTCTCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAAAGCCCCGACAAGTCGGGGCtctt < 1:257682/124‑1 (MQ=255) gcgagggCAGTACTACGCTTTTCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTTTTTGACCAAGAGCCCCGACTTGTCGGGGTTTTTTGTTATCTTCGAGATCTTGAGCCGCGCCt > 2:76277/7‑150 (MQ=255) tactacGCCTCGCTGATGGTCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTc > 2:635898/1‑150 (MQ=255) tacGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTc‑gcg > 2:220187/1‑150 (MQ=255) gCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCTAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAgg < 1:7672/150‑1 (MQ=255) gaAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGc > 2:335008/1‑135 (MQ=255) gaAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGc < 1:335008/135‑1 (MQ=255) aaGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGa < 1:787458/150‑1 (MQ=255) aGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGATT‑ACGATCGCCAGTCACAGGCTGCGATACg > 1:399786/1‑150 (MQ=255) gggAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGc > 1:540489/1‑150 (MQ=255) gggAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGc > 2:316306/1‑150 (MQ=255) ggAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACAAGTCGGGGCt < 2:214842/78‑1 (MQ=255) ggAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACAAGTCGGGGCt > 1:214842/1‑78 (MQ=255) aGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCg < 2:540489/150‑1 (MQ=255) gTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCa > 2:454436/1‑150 (MQ=255) cGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAg < 2:572618/99‑1 (MQ=255) cGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAg > 1:572618/1‑99 (MQ=255) gAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTAC‑ATGGCTGTGCGTGCGGCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTc > 2:419035/1‑150 (MQ=255) gCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAggggg > 2:221603/1‑150 (MQ=255) ncAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCGTGAGCCGCGCCTACAAAAGCAT‑GCCATCACCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGAGGGa > 2:585403/2‑150 (MQ=255) gcgcTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTc > 2:319285/1‑91 (MQ=255) gcgcTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTc < 1:319285/91‑1 (MQ=255) gcTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATAc < 1:756182/150‑1 (MQ=255) aGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGAt > 2:721338/1‑150 (MQ=255) ggCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACg < 2:634965/150‑1 (MQ=255) gCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTAACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACgg > 2:307167/1‑150 (MQ=255) tttGACCACGAGCCCTGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACATGAGCGC‑TCAATAAGCTGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCtt < 1:454436/150‑1 (MQ=255) ttGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCttt < 1:316306/150‑1 (MQ=255) aCCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACtttt > 1:565965/1‑115 (MQ=255) aCCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACtttt < 2:565965/115‑1 (MQ=255) caaaaagCCCCGACAAGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCt > 1:283884/6‑150 (MQ=255) aaaaagCCCCGACAAGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTc > 1:141328/5‑150 (MQ=255) ccGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCGGCttttt > 2:582680/1‑150 (MQ=255) cGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCgg > 2:5013/1‑96 (MQ=255) cGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCgg < 1:5013/96‑1 (MQ=255) | TCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCGGGGCTTTTTGTTATCTGCGAGATCTTGAGCCGCGCCTACAAAGGCTC‑GCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCGGCTTTTT > NC_002947/145983‑146271 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |