Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,435,225 A→T 14.8% L249Q (CTG→CAG)  PP_1256 ← alpha‑ketoglutarate semialdehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,435,2250AT14.8% 84.4 / 5.7 33L249Q (CTG→CAG) PP_1256alpha‑ketoglutarate semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (17/11);  new base T (2/3);  total (19/14)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCCTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGCACTGCTTCACTGCTTG  >  NC_002947/1435079‑1435367
                                                                                                                                                  |                                                                                                                                              
cgcCCTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGtg                                                                                                                                             <  1:387102/150‑1 (MQ=255)
   ccTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCCTACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGtgcca                                                                                                                                          >  1:203194/1‑150 (MQ=255)
    cTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTGGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGtgccat                                                                                                                                         <  1:267561/150‑1 (MQ=255)
         tCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCAc                                                                                                                                    <  1:61430/150‑1 (MQ=255)
           aGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCg                                                                                                                                  >  1:527968/1‑150 (MQ=255)
                    acgaAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCATGGCACTGtgccaagcc                                                                                                                                      >  2:144562/1‑137 (MQ=255)
                    acgaAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCATGGCACTGtgccaagcc                                                                                                                                      <  1:144562/137‑1 (MQ=255)
                      gaAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGcc                                                                                                                       >  2:58598/1‑150 (MQ=255)
                           cTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGGACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGACCAGTAGCATGCCACCGCTGCGCGAGCCAGTGa                                                                                                                  >  2:5382/1‑150 (MQ=255)
                           cTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGa                                                                                                                  >  2:503704/1‑150 (MQ=255)
                                       cTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCt                                                                                                      >  2:126700/1‑150 (MQ=255)
                                               cGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGt                                                                                              >  2:526531/1‑150 (MQ=255)
                                                                   gAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAAt                                                                          <  2:187405/150‑1 (MQ=255)
                                                                         gAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCa                                                                                                             <  1:795699/109‑1 (MQ=255)
                                                                         gAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCa                                                                                                             >  2:795699/1‑109 (MQ=255)
                                                                         gAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCAc                                                                    >  2:120108/1‑150 (MQ=255)
                                                                                   ttGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCg                                                          >  1:652099/1‑150 (MQ=255)
                                                                                     gATCGAACTCATTTAAGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGa                                                        >  2:513451/1‑150 (MQ=255)
                                                                                           aCTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCaa                                                                           >  2:55129/1‑125 (MQ=255)
                                                                                           aCTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCaa                                                                           <  1:55129/125‑1 (MQ=255)
                                                                                            cTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATaa                                                 >  2:429530/1‑150 (MQ=255)
                                                                                             tCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCAGGt                                                                                                                    >  1:439539/1‑82 (MQ=255)
                                                                                             tCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCAGGt                                                                                                                    <  2:439539/82‑1 (MQ=255)
                                                                                              cATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAag                                               <  2:470584/150‑1 (MQ=255)
                                                                                                        tACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAac                                     <  2:652099/150‑1 (MQ=255)
                                                                                                                gATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTc                             <  1:58598/150‑1 (MQ=255)
                                                                                                                  tGGGGTCCGGGCGCGGCTGGGCGGCTTGGCACTGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCg                           <  1:681125/150‑1 (MQ=255)
                                                                                                                       tCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAg                      >  1:209286/1‑150 (MQ=255)
                                                                                                                              gcgcCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGcact               <  1:503704/150‑1 (MQ=255)
                                                                                                                                           ttGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGCACTGCTTCACTGCTTg  >  1:444607/1‑150 (MQ=255)
                                                                                                                                           ttGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGCACTGCTTCACTGCTTg  >  1:628249/1‑150 (MQ=255)
                                                                                                                                           atGGCACTGTGCCAAGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCt                                                                                      <  1:671480/65‑1 (MQ=255)
                                                                                                                                           atGGCACTGTGCCAAGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCt                                                                                      >  2:671480/2‑66 (MQ=255)
                                                                                                                                                acaGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACTTGCGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATaaa                                                <  1:14775/99‑1 (MQ=255)
                                                                                                                                                acaGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATaaa                                                >  2:14775/1‑99 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
CGCCCTGGGTCAGCGAGGCGACGAAACCTTGTGCCAGTGCTTCGGCACGGGCTTGCAGGGCGGCGTCGAACAGGAACACCGGGTTGATCGAACTCATTTCGGCATACACCGGGATGGGCTCCGGGCGCGCCTGGGCGGCTTGGCACAGTGCCATGCCACCGCTGCGCGAGCCGGTGAAACCAACGGCCTTGATACGTGGGTCGCTGACCAGAGCAATGCCCACTTCGCGGCCGGAGCCATAAAGCAGCGAGAACACACCCTCCGGTAAGCCGCACTGCTTCACTGCTTG  >  NC_002947/1435079‑1435367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑