Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,526,622 T→A 11.6% I256N (ATT→AAT)  ddlB → D‑alanine‑‑D‑alanine ligase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,526,6220TA11.6% 127.9 / 6.9 43I256N (ATT→AAT) ddlBD‑alanine‑‑D‑alanine ligase B
Reads supporting (aligned to +/- strand):  ref base T (17/21);  new base A (2/3);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTCCAG  >  NC_002947/1526484‑1526764
                                                                                                                                           |                                                                                                                                              
cccTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTTAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTTCGATGCCAATGGCATCGCAg                                                                                                                                     <  2:359869/150‑1 (MQ=255)
  cTGGTTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCCCCCAGCCTTCGATGCCAATGGCATCGCAgg                                                                                                                                    >  1:146879/1‑150 (MQ=38)
          gccgcACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCg                                                                                                                           >  2:403610/1‑150 (MQ=255)
           ccgcACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATc                                                                                                                                         <  1:349513/135‑1 (MQ=255)
           ccgcACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATc                                                                                                                                         >  2:349513/1‑135 (MQ=255)
                cGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGGGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGa                                                                                                                     <  2:12927/150‑1 (MQ=255)
                 gtgtTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGAc                                                                                                                    <  2:701596/150‑1 (MQ=255)
                   gtTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGt                                                                                                                  <  1:465857/150‑1 (MQ=255)
                          gACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAg                                                                                                           >  1:116881/1‑150 (MQ=255)
                           aCTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGtt                                                                                                                        <  1:184252/136‑1 (MQ=255)
                           aCTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGtt                                                                                                                        >  2:184252/1‑136 (MQ=255)
                                   gCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAgg                                                                                                          >  1:226426/1‑142 (MQ=255)
                                   gCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAgg                                                                                                          <  2:226426/142‑1 (MQ=255)
                                           cATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCT‑GGGGCGGTTGGAc                                                                                                                    >  2:567429/1‑123 (MQ=255)
                                           cATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCT‑GGGGCGGTTGGAc                                                                                                                    <  1:567429/123‑1 (MQ=255)
                                             tCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGAGCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCt                                                                                        >  2:730165/1‑150 (MQ=255)
                                                  aaCGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGgctg                                                                                   >  2:767937/1‑150 (MQ=255)
                                                        aCCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACCCGTAGGGCCGGTTCTGGCTGCTCGaa                                                                             <  1:706594/150‑1 (MQ=255)
                                                           cAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCCCCCAGCCTTCGATGCCAATg                                                                                                                                              <  1:634585/83‑1 (MQ=25)
                                                            aGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCa                                                                         >  2:509184/1‑150 (MQ=255)
                                                                  gCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCg                                                                   >  2:575527/1‑150 (MQ=255)
                                                                       ccGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCa                                                              <  1:767937/150‑1 (MQ=255)
                                                                        cGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAg                                                             >  1:69686/1‑150 (MQ=255)
                                                                          tGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGaa                                                                             >  2:674931/1‑132 (MQ=255)
                                                                          tGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGaa                                                                             <  1:674931/132‑1 (MQ=255)
                                                                              ggCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTTCGATGCCAATGGCATCgcag                                                                                                                                     >  1:161059/1‑69 (MQ=37)
                                                                              ggCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTTCGATGCCAATGGCATCGCAg                                                                                                                                     <  2:161059/72‑1 (MQ=37)
                                                                              ggCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGa                                                       <  1:460623/150‑1 (MQ=255)
                                                                                 tGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAg                                                    <  1:200449/150‑1 (MQ=255)
                                                                                   gATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGtt                                                                                                                        <  2:224314/80‑1 (MQ=255)
                                                                                   gATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGtt                                                                                                                        >  1:224314/1‑80 (MQ=255)
                                                                                    aTAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATc                                                 >  2:464134/1‑150 (MQ=255)
                                                                                       gCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATa                                              <  1:623079/150‑1 (MQ=255)
                                                                                            aaGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTg                                         <  2:611235/150‑1 (MQ=255)
                                                                                                                ccTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGgct                                                                                    <  2:720755/87‑1 (MQ=255)
                                                                                                                ccTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGgct                                                                                    >  1:720755/1‑87 (MQ=255)
                                                                                                                     ccGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTg                <  1:509184/150‑1 (MQ=255)
                                                                                                                      cgcgagc‑gCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGt                                       >  1:156690/1‑126 (MQ=255)
                                                                                                                      cgcgagc‑gCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGt                                       <  2:156690/126‑1 (MQ=255)
                                                                                                                            c‑gcCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTggg         <  2:353894/150‑2 (MQ=255)
                                                                                                                                    gATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTCCAg  <  1:464134/150‑1 (MQ=255)
                                                                                                                                          aTTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGc                                                           >  2:725430/1‑87 (MQ=255)
                                                                                                                                          aTTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGc                                                           <  1:725430/87‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                              
CCCTGGGTACGCCGCACGTGTTCTACGACTATGACGCCAAGTACATCGTCAACGATACCCAGTACCGCATCCCGTGCGGGCTGGATAGCGTCAAGGAACAGGAACTGATCGACCTGACCGCGCGC‑GCCTGCGATGCCATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCCTGGTGCCCATGGCGGCCCGTGCGGCTGGCCTGGACTTCCAG  >  NC_002947/1526484‑1526764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑