| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,980 | A→G | 17.1% | intergenic (‑599/+32) | trmF ← / ← trmE | tRNA uridine 5‑carboxymethylaminomethyl modification protein/GTPase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,980 | 0 | A | G | 17.1% | 83.0 / 12.1 | 41 | intergenic (‑599/+32) | trmF/trmE | tRNA uridine 5‑carboxymethylaminomethyl modification protein/GTPase |
| Reads supporting (aligned to +/- strand): ref base A (20/14); new base G (4/3); total (24/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.35e-01 | |||||||||||
CCGCGAAAGGATGGGTACATCCACAGAGATCTGGTGCTTGTAAGAGCTTCATCACGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAGGTCATCCGAGCTGAATGCTCCTGTGATCTCCCCCAGCGCATGCTGTGCCTGGCGTAAGTCCTCAGCCAGA > NC_002947/4838‑5123 | ccGCGAAAGGATGGGTACATCCACAGAGATCTGGTGCTTGTAAGAGCTTCATCACGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAgcgg < 1:531121/150‑1 (MQ=255) aaGGATGGGTACATCCACAGAGATCTGGTGCTTGTAAGAGCTTCATCACGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCTTAGGGAgcggcttttt < 1:567930/150‑6 (MQ=255) gggTACATCCACAGAGATCTGGTGCTTGTAAGAGCTTCATCACGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTg > 2:624831/1‑150 (MQ=255) ccACAGAGATCTGGTGCTTGTAAGAGCTTCATCACGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCTTAGGGAgcggcttttt > 2:794016/1‑131 (MQ=255) ccACAGAGATCTGGTGCTTGTAAGAGCTTCATCACGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCTTAGGGAgcggcttttt < 1:794016/136‑6 (MQ=255) acaGAGATCTGGTGCTTGTAAGAGCTTCATCACGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCtgtg > 2:795587/1‑150 (MQ=255) agaTCTGGTGCTTGTAAGAGCTTCATCACGGGTCCACCCGCTCCCACAGGTGCTAGGGTCAGCGGCCTGCATGCATCTCCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGGtt > 2:25146/1‑150 (MQ=255) aTCTGGTGCTTGTAAGAGCTTCATCACGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCtt > 1:335624/1‑127 (MQ=255) aTCTGGTGCTTGTAAGAGCTTCATCACGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCtt < 2:335624/127‑1 (MQ=255) gCTTGTAAGAGCTTCATCACGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGa > 1:74297/1‑150 (MQ=255) atcaCGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTgg > 2:623098/1‑117 (MQ=255) atcaCGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTgg < 1:623098/117‑1 (MQ=255) caCGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTg < 1:479842/129‑1 (MQ=255) caCGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTg > 2:479842/1‑129 (MQ=255) aCGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCAGTATATGCAACGAATTTCAGGCAAAGAAAAACCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGgagatcg > 1:454984/1‑147 (MQ=255) cGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAAt < 1:486846/150‑1 (MQ=255) gTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCg > 2:598810/1‑150 (MQ=255) tCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGc < 1:598810/150‑1 (MQ=255) aCCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCa > 2:520467/1‑150 (MQ=255) cccGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCaa > 1:148341/1‑150 (MQ=255) ccGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAg > 2:702626/1‑150 (MQ=255) gCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCa < 2:390975/150‑1 (MQ=255) cTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAg < 1:520467/150‑1 (MQ=255) ccACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCGGGTc > 2:322012/1‑150 (MQ=255) cacaGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAGGTCa > 2:79543/1‑150 (MQ=255) gTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAGGTCATCCGa < 1:624831/150‑1 (MQ=255) cTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGCCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAGGTCATCCGAGCt < 1:79543/150‑1 (MQ=255) gCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAGGTCATCCGAGCTGAATGCTcc < 2:74297/150‑1 (MQ=255) tGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCAGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAGGTCATCCGAGCTGAATGCTCCTGTGAt > 1:139642/1‑150 (MQ=255) tCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAGGTCATCCGAGCTGAATGCTCCTGTGATCTccc > 2:269889/1‑150 (MQ=255) ttCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAGGTCATCCGAGCTGAATGCTCCTGTGATCTCCCCCAGCGCATGCTGTGACt < 1:795587/150‑1 (MQ=255) gCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAg > 2:262351/1‑95 (MQ=255) gCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAg < 1:262351/95‑1 (MQ=255) caagcgCCGCTCCATAGGGAGCGGCGCTTGGCCTTGCGTGGCTGTGCACTGCTCACCGGGGCAGAAGCTGGAGAAAATCCGCCCAAGCAGGTCATCCGAGCTGAATGCTCCTGTGATCTCCCCCAGCGCATGCTGTGCCTGGCGTAAGTc > 2:793393/6‑150 (MQ=255) gCCGCTCCTTAGGGAGCGGCTCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGc > 1:678883/1‑63 (MQ=37) gCCGCTCCTTAGGGAGCGGCTCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGc < 2:678883/63‑1 (MQ=37) gCCGCTCCTTAGGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAGGTCATCCGAGCTGAATGCTCCTGTGATCTCCCCCAGCGCATGCTGTGCCTGGCGTAAGTCCTccg > 2:122781/1‑148 (MQ=255) cGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAGGTCATCCGAGCTGAATGCTCCTGTGATCTCCCCCAGCGCATGCTGTGCCTGGCGTAAGTCCTCAgaa > 1:554624/1‑148 (MQ=255) tCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGga < 2:41932/62‑1 (MQ=255) tCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGga > 1:41932/1‑62 (MQ=255) tCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAGGTCATCCGAGCTGAATGCTCCTGTGATCTCCCCCAGCGCATGCTGTGCCTGGCGTAAGTCCTCAGCCAGa > 2:140681/1‑150 (MQ=255) | CCGCGAAAGGATGGGTACATCCACAGAGATCTGGTGCTTGTAAGAGCTTCATCACGGGTCCACCCGCTCCCACAGGTGCTTGGGTCAGCGGCCTGCATTCATCTTCTGCAACGAATTTCAGGCAAAAAAAAGCCGCTCCCTAAGGAGCGGCGCTTGGCCTTGCGTGGCTGTGGATTACTTGCCGATGCAGAAGCTGGAGAAAATCCGCCCAAGCAGGTCATCCGAGCTGAATGCTCCTGTGATCTCCCCCAGCGCATGCTGTGCCTGGCGTAAGTCCTCAGCCAGA > NC_002947/4838‑5123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |