| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 150,058 | G→A | 12.1% | P298S (CCG→TCG) | PP_0142 ← | ABC transporter permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 150,058 | 0 | G | A | 12.1% | 78.1 / 3.4 | 33 | P298S (CCG→TCG) | PP_0142 | ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base G (14/15); new base A (2/2); total (16/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
TCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGTGAGCAACGGCAGCGAAATCAGCAGGGCCAGTACCCGCGGCACCACCAGCAGCTCCATGGGGTTGAGGCCCAGCGTG > NC_002947/149919‑150203 | tCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGAGCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATcc > 2:751965/1‑150 (MQ=255) ccggttaCCTTGTAGCCTTCCAGTCAGCCGTTGGCGGCGATCAGGAAGTCAAAGAACGGTGCCTTGGCTAGGCCTACTCGGAAGTGCTGCACGCCAATGTCGCTTTCCAGCAGGGACAGTAACAGTGCCGGCGAAATATCCAGTGTCAgg < 2:71391/144‑1 (MQ=255) cGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTCCTTCTGTCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAggg < 1:49227/150‑1 (MQ=255) gCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAggca < 1:751965/150‑3 (MQ=255) gACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCa < 2:670845/150‑1 (MQ=255) cTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCGAGTGCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGGCGGCGAAATATCCAAATGCAGGGCTCATac > 1:362858/1‑150 (MQ=255) cTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATac > 2:297490/1‑150 (MQ=255) aGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCAccg > 1:217430/1‑150 (MQ=255) cTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCAccgccc > 2:591266/1‑150 (MQ=255) ggcggcGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCa < 2:217430/150‑1 (MQ=255) aaaGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGt > 1:139345/1‑150 (MQ=255) gTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGTGAGCAACg < 2:139345/150‑1 (MQ=255) gTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGTGAGCAACg > 1:123352/1‑150 (MQ=255) gTGCATTGTCCAGGCCTCGCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACCTGGCCGGCGAAATCTCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGTGAGCAACg < 2:362858/150‑1 (MQ=255) cTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGTGAGCAACGGCAg > 2:518242/1‑150 (MQ=255) cAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGTGAGCAACGGCAGCGAAAt < 2:510998/150‑1 (MQ=255) ccTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGTGAGCAACGGCAGCGAAATcagc < 2:123352/150‑1 (MQ=255) cTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGACGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGc < 1:342500/105‑1 (MQ=255) cTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGACGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGc > 2:342500/1‑105 (MQ=255) cTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGACGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGc < 1:342450/105‑1 (MQ=255) cTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGACGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGc > 2:342450/1‑105 (MQ=255) cAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGCGCGAAGACCACCGCCCCGCTAATGATGCCGCAGATCATGGCGACAAAGGTGAGCAACGGCAGCGAAATCAGCAGGGc < 1:297490/150‑1 (MQ=255) aGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCaa < 2:767236/94‑1 (MQ=255) aGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCaa > 1:767236/1‑94 (MQ=255) ggAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGAt > 2:231810/1‑97 (MQ=255) ggAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGAt < 1:231810/97‑1 (MQ=255) gTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGTCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGTGAGCAACGGCAGCGAAATCAGCAGGGCCAGTAc < 2:52169/150‑1 (MQ=255) gTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGTGAGCAACGGCAGCGAAATCAGCAGGGCCAGTAc < 1:591266/150‑1 (MQ=255) cAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAg > 2:339860/1‑49 (MQ=255) cAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAg < 1:339860/49‑1 (MQ=255) agcagcGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGTGAGCAACGGCAGCGAAATCAGCAGGGCCAGTACCCGCGGCACCACCAGCAGCTCCATg > 1:202767/1‑150 (MQ=255) gAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGTGAGCAACGGCAGCGAAATCAGCAGGGCCAGTACCCGCGGCACCACCAGCAGCTCCATGGGGTTGAgatc > 2:181783/1‑147 (MQ=255) gCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGTGAGCAACGGCAGCGAAATCAGCAGGGCCAGTACCCGCGGCACCACCAGCAGCTCCATGGGGTTGAGGCCCAGCGTg > 1:694396/1‑150 (MQ=255) ggCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCAGCAGATCATGGCGACAAAGGTGAGCAACGGCAGCGAAATCAGCAGGGCCAGTACCCGCGGCACCACCAGCAGCTCCATggg < 1:566398/131‑1 (MQ=255) ggCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCAGCAGATCATGGCGACAAAGGTGAGCAACGGCAGCGAAATCAGCAGGGCCAGTACCCGCGGCACCACCAGCAGCTCCATggg > 2:566398/1‑131 (MQ=255) | TCGGCGCTGCCGCTGACCTTGAAGCCTTCCAGGCAGCCGATGGCGGCGATCAGGAAGGCAAAGAACGGTGCCTTGGCCAGGCCTACCAGGAAGTGCTGCACGCCAATGTCGCTTTGCAGCAGCGACAGGAACATGGCCGGCGAAATATCCAGTGTCAGGGCGCATACCACCGCCCCGCCAACGATGCCGCAGATCATGGCGACAAAGGTGAGCAACGGCAGCGAAATCAGCAGGGCCAGTACCCGCGGCACCACCAGCAGCTCCATGGGGTTGAGGCCCAGCGTG > NC_002947/149919‑150203 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |