| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,551,324 | G→T | 20.0% | intergenic (+41/+85) | groL → / ← pykA | chaperonin GroEL/pyruvate kinase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,551,324 | 0 | G | T | 20.0% | 76.1 / 11.9 | 35 | intergenic (+41/+85) | groL/pykA | chaperonin GroEL/pyruvate kinase II |
| Reads supporting (aligned to +/- strand): ref base G (17/11); new base T (4/3); total (21/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 | |||||||||||
GGTCTGATGATCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGAT > NC_002947/1551176‑1551465 | ggTCTGATGATCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTcgc < 1:545003/150‑1 (MQ=255)ggTCTGATGATCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTcgc < 1:464580/150‑1 (MQ=255) gatCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCTCGCGAACCg > 2:500930/1‑150 (MQ=255) tCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCTCGCGAACCggg < 1:500930/150‑1 (MQ=255) caccacCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCTCGCGAACCgggg < 2:142372/150‑1 (MQ=255) caccacCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCgggg > 2:326801/1‑150 (MQ=255) caccGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCtt > 1:302247/1‑150 (MQ=255) gACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGcc > 1:669224/1‑150 (MQ=255) gAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCgcag < 1:326801/150‑1 (MQ=255) aaGCCAGCTGCTGGCATGCCTGATCTGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCggg < 1:377441/132‑1 (MQ=255) aaGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCggg > 2:377441/1‑132 (MQ=255) ccAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCTCGCGAACCgggg < 1:703724/110‑1 (MQ=255) ccAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCTCGCGAACCgggg > 2:703724/1‑110 (MQ=255) tgGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGATCCGTGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGa < 2:669224/150‑1 (MQ=255) tGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCtt < 2:424031/150‑1 (MQ=255) ccTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTtgt > 1:738201/1‑150 (MQ=255) cTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTtgtg > 2:536861/1‑150 (MQ=255) tatGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCtgt < 2:738201/150‑1 (MQ=255) tatGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCtgt < 1:536861/150‑1 (MQ=255) gcatgggCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAAc > 2:113061/1‑150 (MQ=255) ggcATGATGTAAGCCAGCCTTACCCCCCGGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGc > 1:744394/1‑150 (MQ=255) gatgTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTAtt < 1:478072/70‑1 (MQ=255) gatgTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTAtt > 2:478072/1‑70 (MQ=255) gccagccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACg > 1:688327/1‑150 (MQ=255) agccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGAc < 1:149973/131‑1 (MQ=255) agccTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGAc > 2:149973/1‑131 (MQ=255) tACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAg > 1:682944/1‑147 (MQ=255) tACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAg < 2:682944/147‑1 (MQ=255) cccccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCtt > 1:718196/1‑150 (MQ=255) cccccTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCtt > 1:302987/1‑150 (MQ=255) aCCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCAt > 1:460317/1‑150 (MQ=255) aTCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGATGCGCTTGAGCCTTGTGCTGTGAGACGAGTGGATCAACCGACCAGCGGATCACCGACATGCAGGATCTTCATGCCATTgg > 1:248003/1‑150 (MQ=255) aaaaaGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGc < 1:113061/150‑1 (MQ=255) aGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAg < 1:628952/92‑1 (MQ=255) aGCCCCGGGTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAg > 2:628952/1‑92 (MQ=255) cccGGTTCTCGCGAACCGGGGCTTTTTTTTTTTTTTTGAGGCCTCTTGGCAGCACAAGGCTTCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGAt > 2:446366/1‑150 (MQ=255) cccGGTTCTCGCGAACAGGGGCTTTTTTATTGTTGTCGGGGCCTCTTCGCAGCACAAGACTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGAt > 1:17717/1‑150 (MQ=255) | GGTCTGATGATCACCACCGAAGCCATGGTTGCCGACCTGCCGGAAGACAAGCCAGCTGCTGGCATGCCTGATATGGGTGGCATGGGTGGCATGGGCGGCATGATGTAAGCCAGCCTTACCCCCCTGCACCATCAAAAAGCCCCGGTTCGCGAGAACCGGGGCTTTTTTATTGCTGTCGGGGCCTCTTCGCAGCACAAGGCTGCGCTTGAGCCTTGTGCTGTGAAACGAGTGGATCAACCGACCAGCGGATCACCGACGTGCAGGATCTTCATGCCATTGGTGCCACCGAT > NC_002947/1551176‑1551465 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |