Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 162,281 A→T 15.8% intergenic (‑315/+59) PP_0153 ← / ← scpC hypothetical protein/propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947162,2810AT15.8% 123.4 / 13.0 50intergenic (‑315/+59)PP_0153/scpChypothetical protein/propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base A (25/17);  new base T (4/4);  total (29/21)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCACTTATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCACGCT  >  NC_002947/162133‑162428
                                                                                                                                                    |                                                                                                                                                   
cGCACTTATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCaa                                                                                                                                                    <  1:17498/150‑1 (MQ=255)
  cACTTATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGc                                                                                                                                                  >  2:777898/1‑150 (MQ=255)
          tGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCTTGCCCGCGaat                                                                                                                                          <  2:348786/150‑2 (MQ=255)
               cGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACAAGCACAGTAGTACAGATTATGATGGTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTATTATGGCCCATTCGCGGGCAATCCCGCGAAGAggac                                                                                                                                     >  2:335420/1‑148 (MQ=255)
                   ttGATTCGAAGAAGGGGATTTTTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGaa                                                                                                                                 <  1:439118/150‑1 (MQ=255)
                      aTTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAg                                                                                                                              >  1:253605/1‑150 (MQ=255)
                       ttCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGaa                                                                                                                                           <  1:222171/136‑1 (MQ=255)
                       ttCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGaa                                                                                                                                           >  2:222171/1‑136 (MQ=255)
                       ttCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCg                                                                                                                                    >  2:527950/1‑143 (MQ=255)
                       ttCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCg                                                                                                                                    <  1:527950/143‑1 (MQ=255)
                                     tttGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGACCAGCGGAAGCAGGgccgc                                                                                                               >  1:224490/1‑150 (MQ=255)
                                            gTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGCAGGGTTTGACTGTTATGGCCCCTTAGAGTAATAGATCGCTAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCg                                                                                                        >  1:575479/1‑150 (MQ=255)
                                               gggAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTAc                                                                                                     >  2:273618/1‑150 (MQ=255)
                                                ggAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTAcc                                                                                                    >  2:590546/1‑150 (MQ=255)
                                                ggAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATCCTGCTCGTGATCATAGGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGACCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTAcc                                                                                                    >  1:418862/1‑150 (MQ=255)
                                                    ggTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACAt                                                                                                <  1:590546/150‑1 (MQ=255)
                                                          aaaGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGaa                                                                                                                                           >  1:625312/1‑101 (MQ=255)
                                                          aaaGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGaa                                                                                                                                           <  2:625312/101‑1 (MQ=255)
                                                              aaCCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGc                                                                                      <  1:777898/150‑1 (MQ=255)
                                                                    cacaGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCa                                                                                <  2:253605/150‑1 (MQ=255)
                                                                     acaGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGCCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGc                                                                                      <  2:622397/143‑1 (MQ=255)
                                                                     acaGTAGTACAGATGATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGc                                                                                      >  1:622397/1‑143 (MQ=255)
                                                                             aCAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGcc                                                                       >  1:493666/1‑150 (MQ=255)
                                                                              cAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGccc                                                                      >  2:259447/1‑150 (MQ=255)
                                                                                aactATGCTGCAAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGCACCACATCCGGATCAGCTGGCCAGCATCCGCCCgg                                                                    >  2:212371/4‑150 (MQ=255)
                                                                                 aTTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCCGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGt                                                                   <  2:206533/150‑1 (MQ=255)
                                                                                       ctgctAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTttcttc                                                             >  1:309139/1‑150 (MQ=255)
                                                                                         gctAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATCGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCa                                                           <  2:575479/150‑1 (MQ=255)
                                                                                            agtagAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGt                                                        <  2:414916/150‑1 (MQ=255)
                                                                                                  ggggTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGt                                                  <  1:259447/150‑1 (MQ=255)
                                                                                                      tGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCa                                              <  1:273618/150‑1 (MQ=255)
                                                                                                        gCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGc                                                                              <  1:411346/116‑1 (MQ=255)
                                                                                                        gCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGc                                                                              >  2:411346/1‑116 (MQ=255)
                                                                                                          gTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTc                                          <  2:224490/150‑1 (MQ=255)
                                                                                                          gTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTc                                          >  1:715967/1‑150 (MQ=255)
                                                                                                            aGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAg                                        <  1:438356/150‑1 (MQ=255)
                                                                                                             gTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAgg                                       >  2:673796/1‑150 (MQ=255)
                                                                                                                   gAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGCTCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTGTTCGTGCCCGCTCAGGGCTTcg                                 >  2:238404/1‑150 (MQ=255)
                                                                                                                    aaGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTcgc                                >  1:500169/1‑150 (MQ=255)
                                                                                                                         tttGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAgg                           >  2:227820/1‑150 (MQ=255)
                                                                                                                                    cggcctcttCGCGGGCTTGCCCGCGAATGGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGt                >  1:280529/8‑150 (MQ=255)
                                                                                                                                       cccATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGCCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATg             >  2:304664/1‑150 (MQ=255)
                                                                                                                                        ctcttCGCGGGCTTGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCa                                                                                >  2:537839/4‑82 (MQ=37)
                                                                                                                                        ctcttCGCGGGCTTGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCa                                                                                <  1:537839/79‑1 (MQ=37)
                                                                                                                                        ccATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATgg            >  2:192626/1‑150 (MQ=255)
                                                                                                                                          cttCGCGGGCTTGCCCGCGAATGGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGgcc          <  2:280529/149‑1 (MQ=255)
                                                                                                                                              gcgGGCTTGCCCGCGAATAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGgcc          <  1:660488/146‑1 (MQ=255)
                                                                                                                                              gcgGGCTTGCCCGCGAATAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGgcc          >  2:660488/1‑146 (MQ=255)
                                                                                                                                                 ggCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCACGc   <  1:335420/150‑1 (MQ=255)
                                                                                                                                                  tcAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGCTTCTTCCAGGTTTTTGGGCCGGCCGACGGCTTGGCGCAGAGTGTGCGTTGTTGGGCCGCCACGCt  >  2:41266/2‑150 (MQ=255)
                                                                                                                                                  gCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCACGCt  >  2:225465/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCACTTATTTGGTGCGTTTTGATTCGAAGAAGGGGATTTGTCAGTCGGGAAGGTCTGAAAGAACCAGCACAGTAGTACAGATTATGCTGCTAGTAGAGGGGTGGCGTAGTGATAGAAGGGTTTGACTGTTATGGCCCATTCGCGGGCAAGCCCGCGAAGAGGCCGGAACAGCGGAAGCAGGGCCGCCGTTCCGTACCACATCCGGATCAGCTGGCCAGCATGCGCCCGGTTTCTTCCAGGTTTTCGTGCCAGCTCAGGGCTTCGCGCAGGATGTGCGGGGTATGGCCGCCACGCT  >  NC_002947/162133‑162428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑