| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,642,327 | G→C | 11.4% | intergenic (‑113/+39) | PP_1442 ← / ← lon‑I | hypothetical protein/DNA‑binding ATP‑dependent protease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,642,327 | 0 | G | C | 11.4% | 101.7 / 4.4 | 35 | intergenic (‑113/+39) | PP_1442/lon‑I | hypothetical protein/DNA‑binding ATP‑dependent protease |
| Reads supporting (aligned to +/- strand): ref base G (14/17); new base C (2/2); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAG > NC_002947/1642188‑1642453 | gggAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCg > 1:327004/1‑150 (MQ=255)gggAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCg > 1:453105/1‑150 (MQ=255)gggAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCACCTGCACGGGTA‑CGCTACCCCTGCAGGcgcg < 1:260073/150‑1 (MQ=38) ggAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCACCTGCACGGGTA‑CGCTACCCCTGCAGGCGCgg < 2:480299/150‑1 (MQ=38) gAACGAGCAGACGAGGAGAGGTCATGGNGGCTCCGAAGGCNGGCGATNGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCACGGGTACGC‑TACCCCTGCAgagatcgg > 1:623269/1‑143 (MQ=255) gTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATCCCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCgg < 1:85882/150‑1 (MQ=255) gtggCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAgg > 2:552472/1‑150 (MQ=255) gCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCACGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTa < 1:186234/150‑1 (MQ=255) tCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGa > 1:52111/1‑150 (MQ=255) ccGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGaa < 1:724696/150‑1 (MQ=255) gAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCACAGCTGGCG‑TACCCGTGCAGGTGCGGGCTGGCCCGCGAAGAGGGCGGTGCAGGCTAGAACa > 1:607186/1‑150 (MQ=255) ggCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCa < 1:3311/150‑1 (MQ=255) cGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTa < 1:409889/118‑1 (MQ=255) cGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTa > 2:409889/1‑118 (MQ=255) gatgatCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCtt > 1:748826/1‑150 (MQ=255) gatCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCaaa < 1:395502/141‑1 (MQ=255) gatCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCaaa > 2:395502/1‑141 (MQ=255) gAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGGCGGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGaaa < 1:48166/150‑1 (MQ=255) gAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAACGCGCTTGGCGaaa > 2:495349/1‑150 (MQ=255) aGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAAt > 1:233549/1‑150 (MQ=255) ggAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAACTCTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATg < 1:662177/150‑1 (MQ=255) ccTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCtt > 1:763403/1‑150 (MQ=255) gtgCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTcgc > 1:68633/1‑150 (MQ=255) tCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTa < 2:763403/150‑1 (MQ=255) ttCGCGGGCAAGCCCGCACCTGCACGGGTA‑CGCTACCCCTGCAGGCGCGGGCTTACTCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTcc > 1:69839/1‑150 (MQ=18) cgAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCCGGATCGACCAGCACTATAGCACCGTCCGCAAGGCGCTTCGCATGAAGCACTTTCATTTCGTGTCGCAGGTAGTCCGGCa < 2:607186/150‑1 (MQ=255) aGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttctt < 2:453105/150‑1 (MQ=255) gCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttcttc < 2:327004/150‑1 (MQ=255) gCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttcttc < 2:68633/150‑1 (MQ=255) cccGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCaaa > 2:427202/1‑95 (MQ=255) cccGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCaaa < 1:427202/95‑1 (MQ=255) cgcgCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGaa < 2:233549/150‑1 (MQ=255) gcgcCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAg < 2:52111/150‑1 (MQ=255) tGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCCTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttcttc < 2:285907/141‑1 (MQ=255) tGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCCTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGttcttc > 1:285907/1‑141 (MQ=255) | GGGAACGAGCAGACGAGGAGAGGTCATGGTGGCTCCGAAGGCTGGCGATGGCCTGAGGATACCCGATGATCGAGGAAATTTGTGCTGCCTGTGCCGGTCCTTTCGCGAGTAAGCCCGCGCCTGCAGGGGTAGCG‑TACCCGTGCAGGTGCGGGCTTGCCCGCGAAGAGGGCGGTGCAGGCTAGAACAGCACTTTCGCCACGTCGGCAAAGCGCTTGGCGAAATGCACTGTCAGGCCTTCGCGCAGGTAGTCCGGCAGTTCTTCGAAG > NC_002947/1642188‑1642453 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |