Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,649,110 G→A 14.8% I664I (ATC→ATT)  PP_1446 ← TonB‑dependent receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,649,1100GA14.8% 60.3 / 4.1 27I664I (ATC→ATT) PP_1446TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base G (10/13);  new base A (2/2);  total (12/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

CAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGTAGCCAACGCTCGCGCCCAAGGTGGTGTAGCCATCGGTGCTGGTTTCG  >  NC_002947/1648962‑1649257
                                                                                                                                                    |                                                                                                                                                   
cAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCgt                                                                                                                                                    <  2:235816/150‑2 (MQ=255)
      tAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATcgc                                                                                                                                              >  1:316618/1‑150 (MQ=255)
         gAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGACCATGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCa                                                                                                                                           <  1:729954/150‑1 (MQ=255)
         gAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCa                                                                                                                                           >  2:570557/1‑150 (MQ=255)
                                     tGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTg                                                                                                               <  1:760599/150‑1 (MQ=255)
                                               gcgcaTTCGCGGGTGAACCCGCTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGGCGGTCAgg                                                                                                     <  2:398828/146‑1 (MQ=255)
                                                    ttCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCAATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTc                                                                                                >  1:500656/1‑150 (MQ=255)
                                                    ttCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCAATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTc                                                                                                >  1:500657/1‑150 (MQ=255)
                                                                   gTTCCTACACCGACCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACgc                                                                                 >  2:633446/1‑150 (MQ=255)
                                                                       cTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTc                                                                                                >  1:10445/1‑131 (MQ=255)
                                                                       cTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTc                                                                                                <  2:10445/131‑1 (MQ=255)
                                                                                          ttGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGt                                                          <  2:413331/150‑1 (MQ=255)
                                                                                              ggTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGaa                                                      <  1:306046/150‑1 (MQ=255)
                                                                                                ttCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGc                                                    >  2:663788/1‑150 (MQ=255)
                                                                                                ttCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGGTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGc                                                    <  2:509233/150‑1 (MQ=255)
                                                                                                   aGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGt                                                 >  2:297817/1‑150 (MQ=255)
                                                                                                    gAAAGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGTa                                                >  2:229916/1‑150 (MQ=255)
                                                                                                                cAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCAACCCACTGGCCCTGGCCAATGTCGAAGCGGTAGCCAACGCTcgc                                    <  2:154254/150‑1 (MQ=255)
                                                                                                                               gCTACGCCCCGGCGCCGGTGCGATAGCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGTAGCCAACGCTCGCGCCCAAGGTGGTGTa                     <  1:429892/150‑1 (MQ=255)
                                                                                                                                      cccGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCTCAACCGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGTAGCCAACGCTCGCGCCCAAGGTGGTGTAGCCATCg              <  1:297817/150‑1 (MQ=255)
                                                                                                                                        cGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGTAGCCAACGCTCGCGCCCAAggtggt                        <  1:741923/138‑1 (MQ=255)
                                                                                                                                        cGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGTAGCCAACGCTCGCGCCCAAggtggt                        >  2:741923/1‑138 (MQ=255)
                                                                                                                                         ggCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGTAGCCAACGCTCGCGCCCAAGGTGGTGTAGCCATCGGTg           >  2:539438/1‑150 (MQ=255)
                                                                                                                                         ggCGCCGGTGCAATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGTAGCCAACGCTCGCGCCCAAGGTGGTGTAGCCATCGGTg           <  2:500657/150‑1 (MQ=255)
                                                                                                                                         ggCGCCGGTGCAATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGTAGCCAACGCTCGCGCCCAAGGTGGTGTAGCCATCGGTg           <  2:500656/150‑1 (MQ=255)
                                                                                                                                                 tGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGTAGCCAACGCTCGCGCCCAAGGTGGTGTAGCCATCGGTGCTGGTTTc   >  2:580675/1‑150 (MQ=255)
                                                                                                                                                 tGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGTAGCCAACGCTCGCGCCCAAGGTGGTGTAGCCATCGGTGCTGGTTTc   <  1:727008/150‑1 (MQ=255)
                                                                                                                                                  gCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGTAGCCAACGCTCGCGCCCAAGGTGGTGTAGCCATCGGTGCTGGTTTCg  <  1:570557/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAGGTGTAGGAGCGGGTTCACCCGCGAATGCGCCGGTTGCCCTACCATCGTATTCGCGGGTGAACCCGTTCCTACACCGATCTTCGTCGCTTGTGGTTCAGAAGGTGGTACGCAACCCGACTTCCACGCTACGCCCCGGCGCCGGTGCGATATCGCGCAGGATCGAACTGGCATAGCGCACGGTCTGGTCGGTCAGGTTTTCGCCGCGCACAAACGCCAGCCACTGGCTCTGGCCAATGTCGAAGCGGTAGCCAACGCTCGCGCCCAAGGTGGTGTAGCCATCGGTGCTGGTTTCG  >  NC_002947/1648962‑1649257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑