Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,683,293	C→G	22.3%	intergenic (+60/‑171)	recJ → / → PP_1478	ssDNA‑specific exonuclease RecJ/xenobiotic reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,683,293	0	C	G	22.3%	48.0 / 11.1	27	intergenic (+60/‑171)	recJ/PP_1478	ssDNA‑specific exonuclease RecJ/xenobiotic reductase
Reads supporting (aligned to +/- strand):  ref base C (11/10);  new base G (2/4);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

TGGGTGGAGTTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGGC  >  NC_002947/1683144‑1683429                                                                                                                                                      |                                                                                                                                         tGGGTGGAGGTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCATGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGccgcccgc                                                                                                                                          >  1:313757/1‑150 (MQ=255)         gTTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCGCGGGCGGCGCt                                                                                                                                  <  2:96679/150‑1 (MQ=255)          ttGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                 <  1:653264/150‑1 (MQ=255)            ggCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCGCGGGCGGCGCTCGa                                                                                                                               <  1:346842/150‑1 (MQ=255)             gCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCgcc                                                                                                                              <  1:713759/150‑3 (MQ=255)                     gCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCGCAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAgg                                                                                                                      >  1:606063/1‑150 (MQ=255)                        ggATGTGAACGAGTTTCGCGGTAATGAGGCCGGGTAGGTGGTGATTGCGCATATGGAGCCGCTCTGAGTTTAGCGGCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGgcgc                                                                                                                   <  2:606063/150‑1 (MQ=38)                                         gcgGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGACGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGa                                                                                                  >  2:377185/1‑150 (MQ=255)                                                           aGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCcaca                                                                                >  1:404217/1‑150 (MQ=255)                                                                        gcATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCcca                                                                   >  1:283787/1‑150 (MQ=255)                                                                           tatGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCCCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGaa                                                                             <  1:437222/137‑1 (MQ=255)                                                                           tatGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCCCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGaa                                                                             >  2:437222/1‑137 (MQ=255)                                                                                aGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTg                                                           >  2:114004/1‑150 (MQ=255)                                                                                      gcTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAg                                                     >  2:207888/1‑150 (MQ=255)                                                                                        tGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGaa                                                                                                 <  2:445702/104‑1 (MQ=255)                                                                                        tGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGaa                                                                                                 >  1:445702/1‑104 (MQ=255)                                                                                                gCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAATCCCTCGCCATCCCACAGAACCTCCCCCCACCATTCTGGTCTAGTCTCATTAAt                                           <  2:283787/150‑1 (MQ=255)                                                                                                     aGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCg                                      <  2:404217/150‑1 (MQ=255)                                                                                                                ttGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCa                           >  1:270464/1‑150 (MQ=255)                                                                                                                    aTCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGa                       >  1:317909/1‑150 (MQ=255)                                                                                                                        tGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCGCCCCGCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGAtgtg                   <  2:313757/150‑1 (MQ=255)                                                                                                                               gggCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGa            <  1:207888/150‑1 (MQ=255)                                                                                                                                        tCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGAt                      >  1:632711/1‑131 (MQ=255)                                                                                                                                        tCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGAt                      <  2:632711/131‑1 (MQ=255)                                                                                                                                        tCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCggg   >  1:593275/1‑150 (MQ=255)                                                                                                                                         cGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCCAACCTTTAATCAAGCCCCTCGCCATCCCACCGACCCGCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGGc  <  1:377185/150‑1 (MQ=38)                                                                                                                                           aGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGa            <  2:315440/138‑1 (MQ=255)                                                                                                                                           aGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGa            >  1:315440/1‑138 (MQ=255)                                                                                                                                                      |                                                                                                                                         TGGGTGGAGTTGGCGTACAAGCTGGATGTGAACGAGTTTCGCGGTAATGAGAGTGTGCAGTTGATGATTGCGCATATGGAGCCGCGCTGAGTTTAGCGTCAGGTGCCTGCCTTGGATCTTGCGCTGGGGCACAAATCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGCTGCAAACCTTTCGGCGAACCCCTCGCCATCCCACAGAACCTCCCCCCACCAATCTGGTCTAGTCTCATTAATGACCGGCACCGGGCCAGGGATGTGCACTTGAGTGTCCGGGC  >  NC_002947/1683144‑1683429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑