Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,707,269 G→C 17.2% intergenic (+45/+97) PP_1501 → / ← PP_1502 hypothetical protein/OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,707,2690GC17.2% 68.9 / 9.3 29intergenic (+45/+97)PP_1501/PP_1502hypothetical protein/OmpA family protein
Reads supporting (aligned to +/- strand):  ref base G (12/12);  new base C (2/3);  total (14/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

GTTCGACACCCAGCAGAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGT  >  NC_002947/1707125‑1707412
                                                                                                                                                |                                                                                                                                               
gTTCGACACCCAGCAGAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCgcgggc                                                                                                                                            <  1:584970/150‑1 (MQ=255)
      cacCCAGCAGAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCt                                                                                                                                      >  2:255827/1‑150 (MQ=255)
       acCCAGCAGAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCCCGCGCGGCGCTc                                                                                                                                     <  1:472484/150‑1 (MQ=255)
         ccAGCAGAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTc                                                                                                                                     >  1:710674/1‑148 (MQ=255)
         ccAGCAGAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTc                                                                                                                                     <  2:710674/148‑1 (MQ=255)
                aaaGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCAGGGGCGAGGGAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcaca                                                                                                                            <  1:301165/150‑1 (MQ=255)
                          ccGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCacaa                                                                                                                  >  2:597158/1‑150 (MQ=255)
                                    ggtggACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGc                                                                                                        <  1:350999/150‑1 (MQ=255)
                                     gtggACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCg                                                                                                       >  1:367922/1‑150 (MQ=255)
                                     gtggACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCCCTCGATCTCACCGGCGGCACAACATTATCGGCg                                                                                                       >  2:676224/1‑150 (MQ=255)
                                         aCAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTTCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCAGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGTCGAACa                                                                                                   >  2:113014/1‑150 (MQ=255)
                                           aaCCGCAAGCGCGAGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACCTTATCGGCGAACaaa                                                                                                 >  2:600304/1‑150 (MQ=255)
                                                cAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtc                                                                                                                                 <  1:119288/113‑1 (MQ=255)
                                                cAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtc                                                                                                                                 >  2:119288/1‑113 (MQ=255)
                                                  aGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGc                                                                                          <  1:597158/150‑1 (MQ=255)
                                                                 gCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATcgcg                                                                           <  2:367922/150‑1 (MQ=255)
                                                                              aaCACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGTTCTCACAGGGGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCttt                                                              <  1:255827/150‑1 (MQ=255)
                                                                                      ggCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTGGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAACCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTagc                                                      <  1:600304/150‑1 (MQ=255)
                                                                                              cAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGt                                                                                   >  2:576333/1‑113 (MQ=255)
                                                                                              cAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGt                                                                                   <  1:576333/113‑1 (MQ=255)
                                                                                                 tGAGGTTTCGGCGCAGCATTTTTGGCGCCTGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAgcg                                           <  1:113014/150‑1 (MQ=255)
                                                                                                 tGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCacaaca                                                                                                                >  2:253314/1‑81 (MQ=255)
                                                                                                 tGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCacaaca                                                                                                                <  1:253314/81‑1 (MQ=255)
                                                                                                      tttCGGCGCAGCATTTTTGGCGCCCGCGACATCGAGCACCGTGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCACGCGTTGATCGCGGGGCTTGTCCTTTCAGCTAGCCATCCTTAGCGCTGaa                                      >  1:498769/1‑150 (MQ=39)
                                                                                                                gcaTTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCAc                            >  2:200271/1‑150 (MQ=255)
                                                                                                                          cgcCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgtggt                  <  2:179886/150‑1 (MQ=255)
                                                                                                                            cctgtgagaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCGCGGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGc                >  1:555170/6‑150 (MQ=38)
                                                                                                                                   gaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTa                                              <  1:699299/113‑1 (MQ=255)
                                                                                                                                   gaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTa                                              >  2:699299/1‑113 (MQ=255)
                                                                                                                                          cgccgcCCGCGCGGCGCTCGATCTCGCGGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGt  <  2:555170/150‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GTTCGACACCCAGCAGAAAGGCCTGACCGGCGTGCTGGTGGACAACCGCAAGCGCGCGGTGCGCTGCATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGT  >  NC_002947/1707125‑1707412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑