| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,718,111 | G→C | 11.6% | L256F (TTG→TTC) | rsmJ → | ribosomal RNA small subunit methyltransferase J |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,718,111 | 0 | G | C | 11.6% | 122.9 / 7.1 | 43 | L256F (TTG→TTC) | rsmJ | ribosomal RNA small subunit methyltransferase J |
| Reads supporting (aligned to +/- strand): ref base G (21/17); new base C (2/3); total (23/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
AAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCAGTAGTCA > NC_002947/1717975‑1718260 | aaGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTg > 1:215436/1‑150 (MQ=255) aGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGc > 2:125694/1‑150 (MQ=255) aGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGCCttcttcgg < 1:715294/150‑8 (MQ=255) caCCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACg > 1:423587/1‑150 (MQ=255) caCCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCAACGCGTTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACg > 1:337086/1‑150 (MQ=255) aCCGGGTGCTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGa > 2:596046/1‑150 (MQ=255) cGGGTGCTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGAtt < 1:596046/150‑1 (MQ=255) gcgcCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGc > 1:148468/1‑126 (MQ=255) gcgcCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGc < 2:148468/126‑1 (MQ=255) gcgcCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCttt > 2:336296/1‑150 (MQ=255) ttaccctcAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAg > 2:411521/8‑150 (MQ=255) gCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAATGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTTTAGTAGCGGCTTTAGCCGCGAACAcc < 2:269936/150‑1 (MQ=255) tcatcGACGGGCCGAAGCCGAGCCATAGCCAGGAGGGTAAGTCGAGCCGGTATGACATCTAACCGAAGAAGGCGTTGAAGGCCTGACCTGCCCCTATCTGGCCCGCTAGCACGATTGTAGCAGCGGCATTAGCCGCGAACACCGACagcc > 2:96703/1‑147 (MQ=255) tcGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCg > 2:790767/1‑150 (MQ=255) gACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGt > 1:316312/1‑150 (MQ=255) ggCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCa < 2:215436/150‑1 (MQ=255) gCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCAt > 2:379318/1‑150 (MQ=255) gAAGCCTAGCCATAGCCTTGAGGGTAAGTCGAGTCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGCCttc < 1:764625/73‑3 (MQ=255) gAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGa < 2:266250/132‑1 (MQ=255) gAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGa > 1:266250/1‑132 (MQ=255) gAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTCAAGGCCttc > 2:764625/1‑71 (MQ=255) aaGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAAc < 2:337086/150‑1 (MQ=255) aaGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAAc < 1:411521/150‑1 (MQ=255) gTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCt < 1:125694/150‑1 (MQ=255) cGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGc < 1:292812/150‑1 (MQ=255) tGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATg < 2:423587/150‑1 (MQ=255) gACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCa > 2:639485/1‑110 (MQ=255) gACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCa < 1:639485/110‑1 (MQ=255) cccGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTAt < 1:790767/150‑1 (MQ=255) ccGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGTCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTAtt < 2:541569/150‑1 (MQ=255) gaagaaGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTc < 1:379318/150‑1 (MQ=255) tcagGCCTTCAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGt < 2:174135/114‑1 (MQ=255) tcagGCCTTCAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGt > 1:174135/3‑116 (MQ=255) gaagGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGc > 2:9027/1‑150 (MQ=255) ggCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGtt < 2:372469/139‑1 (MQ=255) ggCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGtt > 1:372469/1‑139 (MQ=255) ggCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCag > 2:318712/1‑150 (MQ=255) ggCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCag > 2:57479/1‑150 (MQ=255) gCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTcc > 1:701266/1‑124 (MQ=255) gCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTcc < 2:701266/124‑1 (MQ=255) cTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTg > 1:714911/1‑134 (MQ=255) cTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTg < 2:714911/134‑1 (MQ=255) cTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACAATCGAGACCGTTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCagtag > 1:297441/1‑150 (MQ=255) gAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCAGTAGTCa > 1:686209/1‑150 (MQ=255) gAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCCTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCAGTAGTCa < 2:316312/150‑1 (MQ=255) | AAGCCGCTCTGGCGCTGGCCAGCCACCGGGTGGTAGTGAAGCGCCCACGCAAGGCACCGATCATCGACGGGCCGAAGCCGAGCCATAGCCTTGAGGGTAAGTCGAGCCGGTATGACATCTACCCGAAGAAGGCCTTGAAGGCCTGACCTGCCCCTATCTGGCCGGCTAGCACGATTGTAGGAGCGGCTTTAGCCGCGAACACCGACAACGCCGGTGCCATCCACTGCGTCGTCTCCTGCGCGTTAAACCTGCTCCTACAATGACCTGTTATTTCTGCCAGTAGTCA > NC_002947/1717975‑1718260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |