Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,782,578	T→G	9.1%	Q514P (CAG→CCG) ‡	glnD ←	uridylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,782,578	0	T	G	9.1%	165.0 / 10.6	66	Q514P (CAG→CCG) ‡	glnD	uridylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (28/32);  new base G (4/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

GTCGGACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTGAGG  >  NC_002947/1782430‑1782724                                                                                                                                                     |                                                                                                                                                   gTCGGACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCCGGCAGAGGATCAGGCGGCTGTCTCAGCCCGGCAACTGGCGCCGTTCGCAGAACTTCTGCGCGTCCGCTGCACCGATTTCCGAGTGGTCGCCCTg                                                                                                                                                   >  2:107653/1‑150 (MQ=255)  tCGGACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTgg                                                                                                                                                  <  2:60093/150‑1 (MQ=255)  tCGAACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTgg                                                                                                                                                  <  2:565739/150‑1 (MQ=255)    ggAAAAGCCCTTGCGCTGAGCGGTGGTCTGCATCACGAGGTGGTTCTACACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCg                                                                                                                                                >  2:10334/1‑150 (MQ=255)           tCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTg                                                                                                                                         >  2:93706/1‑150 (MQ=255)             cTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGcc                                                                                                                                       >  2:496102/1‑150 (MQ=255)                     gAGCGGTGGTCGACATTACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGt                                                                                                                               <  2:294656/150‑1 (MQ=255)                     gAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGt                                                                                                                               <  1:624899/150‑1 (MQ=255)                       gCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTgg                                                                                                                             >  1:11678/1‑150 (MQ=255)                          gtggtCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGAAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTAc                                                                                                                          <  1:414594/150‑1 (MQ=255)                          gtggtCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATAAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTAc                                                                                                                          >  1:331627/1‑150 (MQ=255)                                gACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCg                                                                                                                    <  2:76461/150‑1 (MQ=255)                                gACATCACCAGGTGGTACTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCg                                                                                                                    >  1:462816/1‑150 (MQ=255)                                  cATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTTCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGGGTTACAGCCCGGc                                                                                                                  <  2:793619/150‑1 (MQ=255)                                    tCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCAGGGCGAccactc                                                                                                                                         >  2:697908/1‑121 (MQ=255)                                    tCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCAGGGCGAccactc                                                                                                                                         <  1:697908/125‑5 (MQ=255)                                     cACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAg                                                                                                               <  2:331627/150‑1 (MQ=255)                                      aCCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAgg                                                                                                              >  2:80357/1‑150 (MQ=255)                                        cAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTa                                                                                                            >  2:467528/1‑150 (MQ=255)                                                tCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCt                                                                                                    >  1:105783/1‑150 (MQ=255)                                                  tGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCg                                                                                                  >  2:88343/1‑150 (MQ=255)                                                         gccagACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTgg                                                                                           <  1:114716/150‑1 (MQ=255)                                                                        ggcTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGa                                                                            <  2:95209/150‑1 (MQ=255)                                                                           tGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTTCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCt                                                                         >  2:600731/1‑150 (MQ=255)                                                                           tGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGc                                                                                                                                        >  2:206691/1‑87 (MQ=255)                                                                           tGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGc                                                                                                                                        <  1:206691/87‑1 (MQ=255)                                                                            gTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCtt                                                                        <  2:457165/150‑1 (MQ=255)                                                                               ccAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCt                                                                     >  1:281953/1‑150 (MQ=255)                                                                               ccAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCAGGGCGAcca                                                                                                                                            >  1:679073/1‑78 (MQ=255)                                                                               ccAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCAGGGCGAcca                                                                                                                                            <  2:679073/79‑2 (MQ=255)                                                                                       gCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCg                                                             <  2:11678/150‑1 (MQ=255)                                                                                              gTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTg                                                                    >  2:497465/1‑136 (MQ=255)                                                                                              gTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTg                                                                    <  1:497465/136‑1 (MQ=255)                                                                                              gTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAAttt                                                      >  2:767151/1‑150 (MQ=255)                                                                                                gCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGa                                                          <  2:463775/144‑1 (MQ=255)                                                                                                gCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGa                                                          >  1:463775/1‑144 (MQ=255)                                                                                                 ccGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTtctc                                                   <  1:467528/150‑1 (MQ=255)                                                                                                  cGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCg                                                  <  1:496102/150‑1 (MQ=255)                                                                                                        cAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCt                                                                     >  1:213706/1‑125 (MQ=255)                                                                                                        cAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCt                                                                     <  2:213706/125‑1 (MQ=255)                                                                                                         aGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACAcca                                           >  2:446225/1‑149 (MQ=255)                                                                                                         aGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCg                                           >  2:574438/1‑150 (MQ=255)                                                                                                             cTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCgt                                       <  1:107653/150‑1 (MQ=255)                                                                                                              ttCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAACTACTCGGACACCGGCgtg                                      <  1:625707/150‑1 (MQ=255)                                                                                                                cTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTa                                    >  2:472006/1‑150 (MQ=255)                                                                                                                 tGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGAAt                                   >  1:124294/1‑150 (MQ=255)                                                                                                                       tCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGc                             >  1:482797/1‑150 (MQ=255)                                                                                                                       tCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGc                             >  1:747023/1‑150 (MQ=255)                                                                                                                           cTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTCCAGACCGGCGATGTATATCATCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTgc                         <  1:93706/150‑1 (MQ=255)                                                                                                                                ccGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAgg                    <  1:574438/150‑1 (MQ=255)                                                                                                                                 cGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTtctc                                                   <  2:327666/118‑1 (MQ=255)                                                                                                                                 cGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTtctc                                                   >  1:327666/1‑118 (MQ=255)                                                                                                                                    tttCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCt                <  1:311162/150‑1 (MQ=255)                                                                                                                                      tCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTtg              <  2:281953/150‑1 (MQ=255)                                                                                                                                         gAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCGCATGCGATCGCAGACGAACTGTTGTTCCTCGGGCACCGGCGTGTATTGCAGCTTGCGCAGGTGCTtgatg           >  1:492450/1‑150 (MQ=255)                                                                                                                                          aagggTCGCCAGGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTtgatga          >  1:688618/4‑150 (MQ=255)                                                                                                                                          aagggTCGCCAGGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTtgagga          >  2:313278/4‑150 (MQ=255)                                                                                                                                           gTGGTCGCCCTGGCGACCCTTGCCGATTTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAg         <  1:763104/150‑1 (MQ=255)                                                                                                                                              gTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGtt      >  2:529749/1‑150 (MQ=255)                                                                                                                                              gTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGtt      <  2:462816/150‑1 (MQ=255)                                                                                                                                              gTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGAATTGCAGCTTGCGCAGGTGCTTGATGAGGtt      <  2:124294/150‑1 (MQ=255)                                                                                                                                               tCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTg     >  2:708935/1‑150 (MQ=255)                                                                                                                                                 gCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTGAg   <  1:708935/150‑1 (MQ=255)                                                                                                                                                 gCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTGAg   <  1:767151/150‑1 (MQ=255)                                                                                                                                                  cccTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTGAgg  <  2:105783/150‑1 (MQ=255)                                                                                                                                                  cccTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTGAgg  <  2:482797/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GTCGGACAAGTCCTTGCGCTGAGCGGTGGTCGACATCACCAGGTGGTTCTGCACCAGCCAGACGATCAGGCGGCTGTCCCAGGCCGGCAACTGGTGCCGTTCGCAGAACTTCTGCGCGTCCACTGCACCGATTTCCGAGTGGTCGCCCTGGCGACCCTTGCCGATGTCGTGGTACAGCCCGGCCAGGTAGATCAGCTCGGGTTTGGGCAGGCGCCCCATGAGCTTGCTGGCCAGCGGGAATTTCTCGGACACCGGCGTGTATTGCAGCTTGCGCAGGTGCTTGATGAGGTTGAGG  >  NC_002947/1782430‑1782724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑