Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,787,003	C→T	13.3%	G264G (GGC→GGT)	tsf →	elongation factor Ts

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,787,003	0	C	T	13.3%	72.1 / 4.3	30	G264G (GGC→GGT)	tsf	elongation factor Ts
Reads supporting (aligned to +/- strand):  ref base C (15/11);  new base T (2/2);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGC‑TCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGT  >  NC_002947/1786856‑1787148                                                                                                                                                    |                                                                                                                                                   gaACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGa                                                                                                                                                  >  1:123814/1‑150 (MQ=255)   aCATGATCAACGGTCGTATCACCAAGTTCAAGGCCGACGACTCACAGAAAAGGCAAGCCTTCGTCATGAACCCGGAGATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCGCCTACTTTGAAGTAGGTGAAg                                                                                                                                                <  1:376817/150‑1 (MQ=255)   aCATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAg                                                                                                                                                <  1:346132/150‑1 (MQ=255)       gATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCAt                                                                                                                                            >  1:655752/1‑150 (MQ=255)                                aaGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCgct                                                                                                                   <  2:655752/150‑1 (MQ=255)                                     cGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGa                                                                                                              >  1:768132/1‑150 (MQ=255)                                                 gAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCa                                                                                                  <  1:568487/150‑1 (MQ=255)                                                                         cccGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAg                                                                               <  2:789488/145‑1 (MQ=255)                                                                         cccGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAg                                                                               >  1:789488/1‑145 (MQ=255)                                                                                      gTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGt                                                             >  1:57341/1‑150 (MQ=255)                                                                                             aGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCa                                                      <  2:57341/150‑1 (MQ=255)                                                                                                tGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAag                                                   >  1:227844/1‑150 (MQ=255)                                                                                                 ggCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAaga                                                  >  2:113196/1‑150 (MQ=255)                                                                                                 ggCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAaga                                                  <  2:227844/150‑1 (MQ=255)                                                                                                      aGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGaa                                                                                                                                      <  1:554217/61‑1 (MQ=255)                                                                                                      aGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGaa                                                                                                                                      >  2:554217/1‑61 (MQ=255)                                                                                                                       ctcgatgccTTCGCCTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGC‑TCACGCGCGCAg                           >  1:777729/8‑150 (MQ=255)                                                                                                                           tttCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCg                                                           <  2:738683/115‑1 (MQ=255)                                                                                                                           tttCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCg                                                           >  1:738683/1‑115 (MQ=255)                                                                                                                           tttCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCaaa                                                    >  1:30750/1‑122 (MQ=255)                                                                                                                           tttCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCaaa                                                    <  2:30750/122‑1 (MQ=255)                                                                                                                             gccTTCGCCTACTTTGAAGTAGGTGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGC‑TCACGCGCGCAGCCTCtt                     <  2:777729/149‑1 (MQ=255)                                                                                                                              ccTTCGCCTACTTTGAAGTAGGTGAAGGCATCAAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGC‑TCACGCGCGCAGCCTCttt                    >  1:154171/1‑150 (MQ=255)                                                                                                                               cTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGTCTGCCCGC‑TAACGCGCGCAGCCTCTTTg                   >  2:498497/1‑150 (MQ=255)                                                                                                                               cTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCGCGCGTGA‑GCGGGCAGCCTCttt                    >  2:19710/1‑149 (MQ=39)                                                                                                                               cTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCGCGCGTGA‑GCGGGCAGCCTCttt                    <  1:19710/149‑1 (MQ=38)                                                                                                                               cTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGC‑TCACGCGCGCAGCCTCTTTg                   >  1:316816/1‑150 (MQ=255)                                                                                                                               cTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGC‑TCACGCGCGCAGCCTCTTTg                   >  1:244042/1‑150 (MQ=255)                                                                                                                                ttCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGC‑TCACGCGCGCAGCCTCTTTGt                  >  1:150301/1‑150 (MQ=255)                                                                                                                                                tAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGC‑TCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGt  <  2:150301/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GAACATGATCAACGGTCGTATCACCAAGTTCAAGGCCGAAGCCTCGCTGAAAGAGCAAGCCTTCGTCATGAACCCGGAAATCAAGGTTGGCGAGCTGGCCAAGAAAGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTTGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGC‑TCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGT  >  NC_002947/1786856‑1787148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑