| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,851,958 | C→G | 100% | H334Q (CAC→CAG) | relA → | ATP:GTP 3'‑pyrophosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,851,958 | 0 | C | G | 100.0% | 131.0 / ‑6.8 | 40 | H334Q (CAC→CAG) | relA | ATP:GTP 3'‑pyrophosphotransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (18/21); minor base A (1/0); total (19/21) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA > NC_002947/1851819‑1852103 | gtgcggtgcgCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTaa < 2:583452/150‑1 (MQ=255) cgTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGa < 1:445585/150‑1 (MQ=255) gCTGGTGCCGGAAATTCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAggg < 2:556524/150‑1 (MQ=255) tGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAAGGCa > 1:255893/1‑150 (MQ=255) tGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGACAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCa > 1:255899/1‑150 (MQ=255) gTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAg < 1:110664/150‑1 (MQ=255) gTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAg < 2:526830/150‑1 (MQ=255) ggAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCt > 2:356740/1‑150 (MQ=255) aTCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAg < 2:231092/150‑1 (MQ=255) cTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCAAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGAt < 2:352832/150‑1 (MQ=255) tGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATc < 1:356740/150‑1 (MQ=255) gcTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTAc < 1:420387/144‑1 (MQ=255) gcTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTAc > 2:420387/1‑144 (MQ=255) tCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTa < 1:708552/135‑1 (MQ=255) tCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTa > 2:708552/1‑135 (MQ=255) tCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACg > 1:617392/1‑150 (MQ=255) tgGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATc < 1:268917/84‑1 (MQ=255) tgGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATc > 2:268917/1‑84 (MQ=255) atatTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTg < 1:429214/144‑1 (MQ=255) atatTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTg > 2:429214/1‑144 (MQ=255) cAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGt < 1:410469/96‑1 (MQ=255) cAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGt > 2:410469/1‑96 (MQ=255) cAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATcc < 2:456311/104‑1 (MQ=255) cAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATcc > 1:456311/1‑104 (MQ=255) cAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGt > 2:134242/1‑150 (MQ=255) cAACCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATACAGTCACTGGCGCTACAAGGGCACCGACGt > 1:599431/1‑150 (MQ=255) aaCCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTa > 2:765916/1‑82 (MQ=255) aaCCCCAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTa < 1:765916/82‑1 (MQ=255) cccAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAggg > 2:108798/1‑137 (MQ=255) cccAAGGAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAggg < 1:108798/137‑1 (MQ=255) ggAGAACGGCTACCGCTCGTTGCAGACTGCGTGAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAg > 1:21043/1‑150 (MQ=255) ggAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAggg > 1:741786/1‑48 (MQ=255) ggAGAACGGCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAggg < 2:741786/48‑1 (MQ=255) ggAGAACGGCTACCGCTCGTTGCAAACAATGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAg > 1:21052/1‑150 (MQ=255) ggCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTg < 2:209082/97‑1 (MQ=255) ggCTACCGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTg > 1:209082/1‑97 (MQ=255) ccGCTCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTa > 1:385893/1‑150 (MQ=255) tCGTTGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACgaa < 1:134242/150‑1 (MQ=255) tGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGGTCTGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaa < 2:21043/150‑1 (MQ=255) tGCAGACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGAACCCCGCCGACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaa < 2:21052/150‑1 (MQ=255) | GTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAA > NC_002947/1851819‑1852103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |