| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,989,188 | G→C | 10.7% | G132A (GGC→GCC) | PP_1780 → | mannosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,989,188 | 0 | G | C | 10.7% | 105.9 / 3.3 | 37 | G132A (GGC→GCC) | PP_1780 | mannosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (18/15); new base C (2/2); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
TGCCGTTGAAAATGCCGACGCCGTTGCCAAGCTTAAATCGTTGGCCGACGCCAGCCAGGTGCTGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGCCTATCCCCAACCTGCTACTGAGCTTTTTGGCGGAATACGAAGGCTTTGGTCGTAGC > NC_002947/1989039‑1989330 | tGCCGTTGAAAATGCCGACGCCGTTGCCAAGCTTAAATCGTTGGCCGACGCCAGCCAGGTGCTGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCcgg > 2:486219/1‑150 (MQ=255) aaaTGCCGACGCCGTTGCCAAGCTTAAATCGTTGGCCGACGCCAGCCAGGTGCTGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGgctgct < 1:324655/150‑1 (MQ=255) gCCGAGGTCGTTTCCAAGCTTAAGTCGTTGGCCGACGCCAGCCAGGTGCTGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTACGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATa < 2:331811/150‑1 (MQ=255) gACGCCGTTGCCAAGCTTAAATCGTTGGCCGACGCCAGCCAGGTGCTGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGgc < 1:782339/139‑1 (MQ=255) gACGCCGTTGCCAAGCTTAAATCGTTGGCCGACGCCAGCCAGGTGCTGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGgc > 2:782339/1‑139 (MQ=255) ccGTTGCCAAGCTTAAATCGTTGGCCGACGCCAGCCAGGTGCTGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGa‑aa < 2:94608/150‑1 (MQ=255) aaGCTTAAATCGTTGGCCGACGCCAGCCAGGTGCTGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTCGAGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAAc > 1:607411/1‑150 (MQ=255) aaGCTTAAATCGTTGGCCGACGCCAGCCAGGTGCTGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAAc > 2:330970/1‑150 (MQ=255) cGACGCCAGCCAGGTGCTGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCAtggt > 2:351418/1‑150 (MQ=255) ccagGTGCTGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGCCGGgcttga > 2:708082/1‑102 (MQ=255) ccagGTGCTGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGCCGGgcttga < 1:708082/105‑4 (MQ=255) tGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCtttt > 2:581398/1‑150 (MQ=255) ttCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGgc < 2:200033/82‑1 (MQ=255) ttCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGgc > 1:200033/1‑82 (MQ=255) acaTTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACACCAAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGcc > 1:451578/1‑150 (MQ=255) ccGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGaaa < 1:287183/150‑1 (MQ=255) ccGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCT‑CTCCGCCACATGGCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTAACTCGACCCCAGCCATGaaa > 1:251048/1‑150 (MQ=255) gAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAg > 1:68881/1‑150 (MQ=255) aTGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGCCTa < 1:330970/150‑1 (MQ=255) ccGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGaaaa > 2:244768/1‑140 (MQ=255) ccGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGaaaa < 1:244768/140‑1 (MQ=255) cGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AAccc < 2:427392/78‑1 (MQ=255) cGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AAccc > 1:427392/1‑78 (MQ=255) gTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTCGA‑AACCCCCAACGCCCAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGCCTATCCCCaa > 2:88537/1‑150 (MQ=255) cccGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGCCTATCCCCAACCt > 2:728502/1‑150 (MQ=255) cGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGCCTATCCCCAACCTGc < 2:716148/150‑1 (MQ=255) ttttGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGcc > 1:745287/1‑133 (MQ=255) ttttGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGcc < 2:745287/133‑1 (MQ=255) aGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGCCTATCCCCAACCTGCTACTGAGCtttt > 1:738211/1‑150 (MQ=255) ggTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGCCTATCCCCAACCTGCTACTGAGCTTTTTGGc > 2:195597/1‑150 (MQ=255) ggTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGCCTATCCCCAACCTGCTACTGAGCTTTTTGGc < 1:351418/150‑1 (MQ=255) gggCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGCCTATCCCCAACCTGCTACTGAGCTTTTTGGCGGaa < 1:728502/150‑1 (MQ=255) gcgcTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCAATGAAAAGCCTATCCACAACCTGCTAGTGGGCTTTTTGGCGGAATACGaa < 2:607411/150‑1 (MQ=255) gagTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGc < 2:510994/92‑1 (MQ=255) gagTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGc > 1:510994/1‑92 (MQ=255) tcagcaGCCCGCCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGCCTATCCCCAACCTGCTACTGAGCttttt > 1:28555/6‑126 (MQ=255) tcagcaGCCCGCCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGCCTATCCCCAACCTGCTACTGAGCttttt < 2:28555/121‑1 (MQ=255) aaGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGCCTATCCCCAACCTGCTACTGAGCTTTTTGGCGGAATACGAAGGCTTTGGTCGTAGc > 1:633609/1‑150 (MQ=255) | TGCCGTTGAAAATGCCGACGCCGTTGCCAAGCTTAAATCGTTGGCCGACGCCAGCCAGGTGCTGGTGTCCGGGTTCCACATTGCCGAACATGTCCCGTTCCCGGTTTTGCAACAGCTGGTAGGGCAGGCGCTTAGAGTACTCAAGCCCGGCGGGCTGCTGATACTGGA‑AACCCCCAACGCCGAAAACCTCATGGTGGGGACCAACAGCTTTTACCTCGACCCCAGCCATGAAAAGCCTATCCCCAACCTGCTACTGAGCTTTTTGGCGGAATACGAAGGCTTTGGTCGTAGC > NC_002947/1989039‑1989330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |