| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,019,126 | C→A | 9.1% | L120M (CTG→ATG) ‡ | PP_1797 → | HlyD family secretion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,019,126 | 0 | C | A | 9.1% | 134.2 / 3.0 | 44 | L120M (CTG→ATG) ‡ | PP_1797 | HlyD family secretion protein |
| Reads supporting (aligned to +/- strand): ref base C (22/18); new base A (2/2); total (24/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01 | |||||||||||
TCGTCAAAGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTCAACC > NC_002947/2018986‑2019273 | tCGTCAAAGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCAGGCGTGcct < 1:429984/150‑2 (MQ=255) cGTCAAAGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGa < 1:314261/150‑1 (MQ=255) gCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCtt < 1:569890/150‑1 (MQ=255) aTGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTgg > 1:612183/1‑150 (MQ=255) gCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGAc > 1:783941/1‑150 (MQ=255) ccGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTg > 2:245859/1‑145 (MQ=255) ccGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTg < 1:245859/145‑1 (MQ=255) ccGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTc > 2:756940/1‑150 (MQ=255) cgATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATCAGTCGCAGCTCAGCGCCGAGTATGAGTTCCTCTTCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGAt < 1:377265/150‑1 (MQ=255) gATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTAcc > 2:357522/1‑150 (MQ=255) cTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCt > 2:545918/1‑150 (MQ=255) ttGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACAAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGAcc < 1:45428/150‑1 (MQ=255) tGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCgcgcgc > 1:56749/1‑150 (MQ=255) aGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCaagaag > 1:389281/1‑150 (MQ=255) tCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAgcg < 2:56749/150‑1 (MQ=255) gCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGaa > 2:485823/1‑115 (MQ=255) gCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGaa < 1:485823/115‑1 (MQ=255) aGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGt < 2:385324/150‑1 (MQ=255) ccGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGagc < 2:612183/150‑1 (MQ=255) tATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGcagcag < 1:756940/150‑1 (MQ=255) tATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCAGGCGTGc > 1:52041/1‑59 (MQ=255) tATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCAGGCGTGc < 2:52041/59‑1 (MQ=255) tGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGgt > 1:617338/1‑150 (MQ=255) ccACCCGCAACCAACTGATTGTTGCCGGGTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCa > 1:107855/1‑150 (MQ=255) ccGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCaagaa < 1:158441/118‑1 (MQ=255) ccGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCaagaa > 2:158441/1‑118 (MQ=255) ccGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGc > 2:482932/1‑150 (MQ=255) cGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGcc > 2:341433/1‑150 (MQ=255) aTTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCg > 2:342563/1‑150 (MQ=255) ttgCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGc > 1:505138/1‑150 (MQ=255) gCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCTGCCACGATTCGTTGCTg < 1:341433/150‑1 (MQ=255) ccGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAgg < 1:141890/128‑1 (MQ=255) ccGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAgg > 2:141890/1‑128 (MQ=255) gTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCGCGCCACGATTCGTTGCTGGGCg > 1:360169/1‑150 (MQ=255) ataAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGaaa > 2:782631/1‑150 (MQ=255) aaaGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGCTCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCGCGCCACGATTCGTTGCTGGGCGAAAtc > 1:368840/1‑150 (MQ=255) aaaGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGcagcag < 2:4285/111‑1 (MQ=255) aaaGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGcagcag > 1:4285/1‑111 (MQ=255) tcggCACGCAGGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCg > 1:695530/3‑150 (MQ=255) gagGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCg < 1:482932/150‑1 (MQ=255) gagGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCg < 1:357522/150‑1 (MQ=255) agGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCgg < 1:782631/150‑1 (MQ=255) cGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTCAAc < 1:343507/150‑1 (MQ=255) gCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTCAAcc > 2:463127/1‑150 (MQ=255) | TCGTCAAAGAGCTGCATGCCCGCGATGGGGACCTGGTGAAGAAGGGCGATCCGCTGATTGTGCTGGATGAGTCGCAGCTCAGCGCCGAGTATGAGTCCACCCGCAACCAACTGATTGTTGCCCGTTATAAAGAGGCACGCCTGCGTGCCGAGCGTGACGGCTTGGACTCGATCCCGTCGGTGATAATGGAAGGTACCGATTCTGACCGCGCGCAAGAAGCGCTGGCAGGTGAGCAGCAGGTGTTCAAGGCCCGCCACGATTCGTTGCTGGGCGAAATCTCGGTCAACC > NC_002947/2018986‑2019273 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |