| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,260,786 | G→A | 11.8% | G444D (GGC→GAC) | fimV → | motility protein FimV |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,260,786 | 0 | G | A | 11.8% | 81.9 / 4.1 | 34 | G444D (GGC→GAC) | fimV | motility protein FimV |
| Reads supporting (aligned to +/- strand): ref base G (13/17); new base A (2/2); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01 | |||||||||||
GTTCCAGCATCCTGGTGCTGGCACTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACCACAAGCAGACCCGC > NC_002947/2260639‑2260926 | gTTCCAGCATCCTGGTGCTGGCACTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTcggcg > 1:135856/1‑150 (MQ=255) aGCATCCTGGTGCTGGCACTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTc > 1:309707/1‑150 (MQ=255) ggTGCTGGCACTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGACGCCGCCGAAGctct < 1:412197/150‑4 (MQ=255) ggtGGCACTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGa < 2:492389/148‑1 (MQ=255) cACTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCgg < 1:305953/150‑1 (MQ=255) ctgctgctgTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGgt < 2:135856/150‑1 (MQ=255) gctgctgTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGaa > 2:232352/1‑133 (MQ=255) gctgctgTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGaa < 1:232352/133‑1 (MQ=255) ctgTGGCTGCTGGCGCGCAAACGCAAGGCCCGGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAAcc < 1:730572/150‑1 (MQ=255) tgctgGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGc < 2:309707/150‑1 (MQ=255) ggcgggggcgcAAACGCAAGGCCCAGCAAGAAGAAGAGAAGCACCTGCGCATGGCGGGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGc < 2:264786/143‑1 (MQ=255) tgGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAACACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCgg > 1:645091/1‑150 (MQ=255) cccAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCgg > 1:450125/1‑150 (MQ=255) agCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAAc < 1:170763/111‑1 (MQ=255) agCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAAc > 2:170763/1‑111 (MQ=255) cTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTc > 1:784440/1‑75 (MQ=255) cTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTc < 2:784440/75‑1 (MQ=255) gcgcGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCa < 1:787520/150‑1 (MQ=255) cgGGCGCTGGAAGAAGAGCAGGGCGCAGGTGTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGaa < 1:129491/150‑1 (MQ=255) gcTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGt < 2:450125/150‑1 (MQ=255) agaagaGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTg < 2:8695/133‑1 (MQ=255) agaagaGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTg > 1:8695/1‑133 (MQ=255) gagCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGgca < 2:731260/150‑1 (MQ=255) agCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGgcag > 2:100335/1‑150 (MQ=255) gggCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCAcc > 1:153176/1‑150 (MQ=255) tttCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGa > 2:447978/1‑150 (MQ=255) tCGACCGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAGAAGGGCCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAAc < 2:626413/150‑1 (MQ=255) aCAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACcac > 1:37792/1‑150 (MQ=255) ccggctCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACCACAAGc > 2:75342/6‑150 (MQ=255) atCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACCACAAGCAGAc < 2:751216/150‑1 (MQ=255) ggggCTTCGACGCCGCCGAAGCTCTCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGc > 2:205254/4‑100 (MQ=37) ggggCTTCGACGCCGCCGAAGCTCTCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGc < 1:205254/97‑1 (MQ=37) ggggCTTCGACGCCGCCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTACCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACCACAAGCAGACCCGc > 2:452368/4‑150 (MQ=255) cTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTgg > 2:208177/1‑95 (MQ=255) cTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTgg < 1:208177/95‑1 (MQ=255) | GTTCCAGCATCCTGGTGCTGGCACTGCTGCTGTGGCTGCTGGCGCGCAAACGCAAGGCCCAGCAAGAAGCCGAGAAGCACCTGCGCATGGCGCGGGCGCTGGAAGAAGAGCAGGGCGCAGGTTTCGACAGCGATATCGAGAGCTTCGGCGGCGTCGAAGCCCCCGAGCCGGGTGTAACCCTGTCGCCGGCTGTGGTTGCCGCTTCGGCTGCAGCTGCCGTCGCTGCGGAAAAAGTGGCAGAACCGGTCGCCGAGGCAGCACCCGAGCCTGAACCACAAGCAGACCCGC > NC_002947/2260639‑2260926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |