| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,318,705 | C→T | 15.2% | R302C (CGC→TGC) | PP_2039 → | acyl‑CoA dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,318,705 | 0 | C | T | 15.2% | 69.2 / 6.9 | 33 | R302C (CGC→TGC) | PP_2039 | acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (15/13); new base T (3/2); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.41e-01 | |||||||||||
ACCTTGCTGGGGCTGGCCGGCCTGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGCCGGGCTGCTGCGT > NC_002947/2318564‑2318854 | aCCTTGCTGGGGCTGGCCGGCCTGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCGTGCAGCATg < 2:500198/150‑1 (MQ=255) ttGCTGGGGCTGGCCGGCCTGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTc < 2:324045/150‑1 (MQ=255) gggCTGGCCGGCCTGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCAAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGcc > 1:263681/1‑150 (MQ=255) tGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACAGCAGCGGTGa < 2:683389/150‑1 (MQ=255) aCTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGa > 2:409956/1‑150 (MQ=255) ccTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCcgg < 1:85310/150‑1 (MQ=255) tctcGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCcggcg > 1:746452/1‑150 (MQ=255) aCTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATcc > 2:640928/1‑150 (MQ=255) aCTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATcc > 1:588264/1‑150 (MQ=255) tGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAACATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCAc > 2:667573/1‑150 (MQ=255) ccGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCa > 1:555760/1‑150 (MQ=255) cGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAg < 1:19389/150‑1 (MQ=255) gcgcAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGcccc < 1:640928/150‑1 (MQ=255) cAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGAt < 2:344012/150‑1 (MQ=255) gTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGt > 2:214188/1‑105 (MQ=255) gTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGt < 1:214188/105‑1 (MQ=255) ttCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAg < 1:409956/150‑1 (MQ=255) ttCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCACATGATCGCAACCATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAg < 1:179253/150‑1 (MQ=255) cAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTc < 1:572921/150‑1 (MQ=255) cAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTc < 2:555760/150‑1 (MQ=255) cAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTc > 2:572921/1‑150 (MQ=255) tCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCgg > 1:392742/1‑150 (MQ=255) ccATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAACATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGgaga < 1:667573/150‑1 (MQ=255) ccATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGa > 2:282612/1‑150 (MQ=255) cATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCa > 1:576821/1‑94 (MQ=255) cATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCa < 2:576821/94‑1 (MQ=255) aTGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATa > 1:268967/1‑150 (MQ=255) tGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATaa > 2:563557/1‑149 (MQ=255) ccACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACAcg > 2:216388/1‑150 (MQ=255) cATGCTGCGTGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAatat > 1:110252/1‑59 (MQ=38) cATGCTGCGTGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAatat < 2:110252/59‑1 (MQ=255) cATGCTGCGTGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCTCAGCGCCGATCTCGCCGTGCACAAACACGGCGGTgaccgc > 2:330009/1‑145 (MQ=255) cATGCTGCGTGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGCCggg > 2:240166/1‑150 (MQ=255) cGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGCCGGGCTGCTGCGt < 2:588264/150‑1 (MQ=255) | ACCTTGCTGGGGCTGGCCGGCCTGGCACTGAACCTCTCGATCGACTATGCCCGCACGCGCAAGCAGTTCGGCCAGCCTATCGCCATGTTCCAGTCGATCAACCATATGATCGCCGACATGGCCACCGAGCTGCATGCTGCACGCAGCATGGTCTACGCCACGGCAGCGGTGAATGACGCCGGCGGCAATATCCGCACCCAGGCCCCGATGTGCAAGCTGTTCGTTTCGGAGACGGCCTTCCGCATCGCCGATCGCGCCGTGCAGATACACGGCGGTGCCGGGCTGCTGCGT > NC_002947/2318564‑2318854 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |