Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,410,403 A→G 8.5% L363P (CTG→CCG)  acnA‑I ← aconitate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,410,4030AG 8.5% 134.1 / 2.7 47L363P (CTG→CCG) acnA‑Iaconitate hydratase
Reads supporting (aligned to +/- strand):  ref base A (18/25);  new base G (2/2);  total (20/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

TCCAGGCGGCCAACTTCTTTGGCCAGCGGTTTGGGCTGCAGTTCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACGGTCGCTTCGGGGCGGCCCGAGAGGCGCAGATAGTCCAGAGTGACTTGGTCGACCGGGAA  >  NC_002947/2410256‑2410542
                                                                                                                                                   |                                                                                                                                           
tCCAGGCGGCCAACTTCTTTGGCCAGCGGTTTGGGCTGCAGTTCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGc                                                                                                                                           <  2:439983/150‑1 (MQ=255)
   aGGCGGCCAACTTCTTTGGCCAGCGGTTTGGGCTGCAGTTCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCgtg                                                                                                                                        <  2:684943/150‑1 (MQ=255)
    ggcggcCAACTTCTTTGGCCAGCGGTTTGGGCTGCAGTTCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCgtgt                                                                                                                                       >  1:762316/1‑150 (MQ=255)
        gcCAACTTCTTTGGCCAGCGGTTTGGGCTGCAGTTCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCt                                                                                                                                   >  2:532703/1‑150 (MQ=255)
        gcCAACTTCTTTGGCCAGCGGTTTGGGCTGCAGTTCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCt                                                                                                                                   >  2:135080/1‑150 (MQ=255)
          cAACTTCTTTGGCCAGCGGTTTGGGCTGCAGTTCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGa                                                                                                                                 >  1:193168/1‑150 (MQ=255)
               tcttTGGCCAGCGGTTTGGGCTGCAGTTCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGAc                                                                                                                            <  1:484109/150‑1 (MQ=255)
                  ttGGCCAGCGGTTTGGGCTGCAGTTCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGc                                                                                                                         <  1:526544/150‑1 (MQ=255)
                            gTTTGGGCTGCAGTTCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCgg                                                                                                               <  2:762316/150‑1 (MQ=255)
                                        gTTCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCcaca                                                                                                   >  2:77333/1‑150 (MQ=255)
                                         ttCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTc                                                                                                                       >  2:659563/1‑129 (MQ=255)
                                         ttCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTc                                                                                                                       <  1:659563/129‑1 (MQ=255)
                                         ttCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGccgtca                                                                                                  <  1:80722/150‑5 (MQ=255)
                                             aTGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCa                                                                                                                                             <  2:493304/103‑1 (MQ=255)
                                             aTGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCa                                                                                                                                             >  1:493304/1‑103 (MQ=255)
                                                aaGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCtgt                                                                                           <  1:135080/150‑1 (MQ=255)
                                                aaGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCtgt                                                                                           <  1:532703/150‑1 (MQ=255)
                                                     gTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCGGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCtt                                                                                      >  1:358078/1‑150 (MQ=255)
                                                     gTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCGGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCtt                                                                                      >  1:756922/1‑150 (MQ=255)
                                                     gTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCtt                                                                                      <  1:784375/150‑1 (MQ=255)
                                                         aaGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCCTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGgcgc                                                                                                       <  1:186741/129‑1 (MQ=255)
                                                         aaGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCCTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGgcgc                                                                                                       >  2:186741/1‑129 (MQ=255)
                                                             cTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCt                                                                                                                                   >  2:454103/1‑97 (MQ=255)
                                                             cTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCt                                                                                                                                   <  1:454103/97‑1 (MQ=255)
                                                                   tGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTCGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGa                                                                        <  1:77333/150‑1 (MQ=255)
                                                                    gACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGAc                                                                       <  2:157185/150‑1 (MQ=255)
                                                                        tGGCCCAGCGCTACGGGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGt                                                                   <  1:113790/150‑1 (MQ=255)
                                                                            ccAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCa                                                               >  1:735649/1‑150 (MQ=255)
                                                                             gaGCGCGACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCaa                                                              >  1:33213/2‑149 (MQ=255)
                                                                               gcgcTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCGGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACgg                                                            <  2:756922/150‑1 (MQ=255)
                                                                               gcgcTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCGGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACgg                                                            <  2:358078/150‑1 (MQ=255)
                                                                               gcgcTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGCCCAGTTGCActt                                                            <  2:30350/150‑3 (MQ=255)
                                                                                         gTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCAGCATCGCTTCtgg                                                  <  2:33213/150‑1 (MQ=255)
                                                                                                          gCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACGGTCGCTTCGGGGCGGCCCGAGAGGCGCa                                 <  1:250363/150‑1 (MQ=255)
                                                                                                           cccGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACGGTCGCTTCGGGGCGGCCCGAGAGGCGCAg                                >  2:347923/1‑150 (MQ=255)
                                                                                                              ggCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCa                                                               <  2:790192/116‑1 (MQ=255)
                                                                                                              ggCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCa                                                               >  1:790192/1‑116 (MQ=255)
                                                                                                                      tggctTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACGGTCGCTTCGGGGCGGCCCGAGAGGCGCAGATAGTCCagag                     >  1:248065/1‑150 (MQ=255)
                                                                                                                         ctTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACGGTCGCTTCGGGGCGGCCCGAGAGGCGCAGATAGTc                          >  2:668574/1‑142 (MQ=255)
                                                                                                                         ctTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACGGTCGCTTCGGGGCGGCCCGAGAGGCGCAGATAGTc                          <  1:668574/142‑1 (MQ=255)
                                                                                                                         ctTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACGGTCGCTTCGGGGCGGCCCGAGAGGCGCAGATAGTCCAGAGTGa                  <  1:347923/150‑1 (MQ=255)
                                                                                                                          ttCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACGGTCGCTTCGGGGCGGCCCGAGAGGCGCAGATAGTCCAGAGTGAc                 >  2:191041/1‑150 (MQ=255)
                                                                                                                                gtcgtcCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTACACGGTCGCTTCGGGGCGGCCCGAGAGGCGCAGATAGTCCAGAGTGACTTGGTc           >  2:630545/1‑150 (MQ=255)
                                                                                                                                       aTGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGcc                                                                                               >  2:488737/1‑59 (MQ=255)
                                                                                                                                       aTGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGcc                                                                                               <  1:488737/59‑1 (MQ=255)
                                                                                                                                         gTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCTTGGTCGCTTCGGGGCGGCCCGAGAGGCGCAGATAGTCCAGAGTGACTTGGTCGACCGGgaa  <  2:193168/150‑1 (MQ=255)
                                                                                                                                         gTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACGGTCGCTTCGGGGCGGCCCGAGAGGCGCAGATAGTCCAGAGTGACTTGGTCGACCGGgaa  <  2:245502/150‑1 (MQ=255)
                                                                                                                                                gCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACGGTCGCTTCGGGGCGGCCCGAGAGGCGCAGATAGTCCAGAGTGACTTGGTc           >  2:715674/1‑134 (MQ=255)
                                                                                                                                                gCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACGGTCGCTTCGGGGCGGCCCGAGAGGCGCAGATAGTCCAGAGTGACTTGGTc           <  1:715674/134‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
TCCAGGCGGCCAACTTCTTTGGCCAGCGGTTTGGGCTGCAGTTCGATGAAGTGGTCGAAGGCTTGGCTGACCTGGCCCAGCGCTACGCGGTCCTGCGGCCGTTTGGGCCCGGCCAGGCTGGCTTCGACGTCGTCCATGTCCAGGGCCAGCGTGTCGCTGAATGACGGCTCCTGGCCGGGCAGGCGCCACATGCCCTGTGCCTTGCAGTACTGCTCGACCAGTTGCACGGTCGCTTCGGGGCGGCCCGAGAGGCGCAGATAGTCCAGAGTGACTTGGTCGACCGGGAA  >  NC_002947/2410256‑2410542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑