| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 195,559 | C→A | 24.7% | T355T (ACC→ACA) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 195,559 | 0 | C | A | 24.7% | 86.4 / 16.4 | 41 | T355T (ACC→ACA) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (14/16); new base A (6/4); total (21/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAA > NC_002947/195420‑195705 | cGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAAtt > 1:601558/1‑150 (MQ=37) cccTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGa < 2:701938/150‑1 (MQ=255) aaCGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAAt < 2:634495/150‑1 (MQ=255) atcaCTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCt < 1:550115/150‑1 (MQ=255) atcaCTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGATAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCt > 2:518213/1‑150 (MQ=255) ggCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAc < 2:583406/150‑1 (MQ=38) ggCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGATAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAc < 1:518213/150‑1 (MQ=255) acaaCCCAGGCGAGCGTCGAATGCCAGACCCCGGCCAATGAGGTCTATAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GAc < 1:170912/148‑1 (MQ=11) gCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCaa < 1:194810/95‑1 (MQ=38) gCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCaa > 2:194810/1‑95 (MQ=38) tttCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAAACCTCGCCCACTCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACcgc < 2:592689/150‑1 (MQ=14) cAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGAcc > 1:81570/1‑150 (MQ=255) cAGACCACGGCAAATAACGTCTACAACAAAGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGAcc > 2:628684/1‑150 (MQ=11) aGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCTACGGGCGGCAATTTCGAGCAACTGACTCCGAATCCGACCCCGGGTCAGACACCGA‑CAACAGGATCGGTCGACACCACCACCGCGAccc > 1:123878/1‑150 (MQ=11) aGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg > 2:270378/1‑80 (MQ=255) aGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAg < 1:270378/80‑1 (MQ=255) cccGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCCAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGAGACCCTGAc > 2:6310/1‑150 (MQ=255) cGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGAACACGAACAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCTAGCCCGTc > 1:320609/1‑150 (MQ=255) gTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCg < 1:628684/150‑1 (MQ=18) cTACAACAACGGTTCGACCGTCAGCATCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTg < 2:693103/133‑1 (MQ=255) cTACAACAACGGTTCGACCGTCAGCATCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTg > 1:693103/1‑133 (MQ=255) aacaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCAcc > 1:23351/1‑150 (MQ=39) acaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAAc > 1:648702/1‑60 (MQ=38) acaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAAc < 2:648702/60‑1 (MQ=38) acaacGGTTCGACCGTCAGCGTCACCAACGAGAACGCCACCGCCGGTAATTCCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑AAATCGGTCGACAGCCGCACCGCGACCCTGACAGCGAGCCAGTCgagcacaa > 2:379757/1‑143 (MQ=11) gTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg < 2:601558/150‑1 (MQ=38) gTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATATCGCGCAACAGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACGCGGTCGACACCACCACCACGGCCCTGACGGCGAGCCCGTCGCTCACCGAAggcg > 2:29321/1‑150 (MQ=11) ttCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcgg > 1:348121/1‑150 (MQ=12) cGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGAt < 2:81570/150‑1 (MQ=40) gCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACtt < 2:480960/150‑1 (MQ=12) aCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACAcc > 2:321561/1‑150 (MQ=17) aCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGCCGACACCACTACCGGCACCCTGACGCCGAGCCCGTCGGCCACCACAGCCGGCGAGATCACTTAcccc > 2:110299/1‑150 (MQ=11) aTCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGAcaccaccac > 2:429237/1‑92 (MQ=255) aTCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGAcaccaccac < 1:429237/92‑1 (MQ=255) tCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGa < 2:23351/150‑1 (MQ=255) tCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGa > 1:470444/1‑150 (MQ=18) gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAcc < 2:420816/150‑1 (MQ=12) gaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCt > 1:132429/1‑150 (MQ=12) aCGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGGGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa < 1:321561/150‑1 (MQ=12) cacaGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCaa > 1:228979/1‑150 (MQ=14) cACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa < 2:168989/146‑1 (MQ=255) cACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa > 1:168989/1‑146 (MQ=255) | CGGTGACCGTGACCCTGTCCAACGGCCAGGTCATCACTGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAA > NC_002947/195420‑195705 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |