Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,688,176 G→T 12.4% L22M (CTG→ATG)  PP_2355 ← two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,688,1760GT12.4% 91.3 / 2.4 32L22M (CTG→ATG) PP_2355two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base G (13/15);  new base T (2/2);  total (15/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGACCTTGGGCAGTTTCAGGTCGAGTAACAGCACGGCAGGGTTGCCGTCATCGCGCTCGGCATAGGCGTTGCGGCGCAGCAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCAGTGTTTCGGGGTAGGGTGAAGTGGAAGCTGGCGCCTTGGTCGACCTGGCCAGTGGCCCAG  >  NC_002947/2688027‑2688324
                                                                                                                                                     |                                                                                                                                                    
gTCGACCTTGGGCAGTTTCAGGTCGAGTAACAGCACGGCAGGGTTGCCGTCATCGCGCTCGGCATAGGCGTTGCGGCGCAGCAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAg                                                                                                                                                      >  1:412700/1‑150 (MQ=255)
       ttGGGCAGTTTCAGGTCGAGTAACAGCACGGCAGGGTTGCCGTCATCGCGCTCGGCATAGGCGTTGCGGCGCAGCAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCcagc                                                                                                                                               <  2:608620/150‑1 (MQ=255)
             aGTTTCAGGTCGAGTAACAGCACGGCAGGGTTGCCGTCATCGCGCTCGGCATAGGCGTTGCGGCGCAGCAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTc                                                                                                                                         >  2:125436/1‑150 (MQ=255)
                           tAACAGCACGGCAGGGTTGCCGTCATCGCGCTCGGCATAGGCGTTGCGGCGCAGCAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCg                                                                                                                           <  2:412700/150‑1 (MQ=255)
                            aaCAGCACGGCAGGGTTGCCGTCATCGCGCTCGGCATAGGCGTTGCGGCGCAGCAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCgg                                                                                                                          <  1:125436/150‑1 (MQ=255)
                            aaCAGCACGGCAGGGTTGCCGTCATCGCGCTCGGCATAGGCGTTGCGGCGCAGCAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCgg                                                                                                                          <  1:785816/150‑1 (MQ=255)
                            aaCAGCACGGCAGGGTTGCCGTCATCGCGCTCGGACTGGGCGTTGCGGCGCAGCAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCgg                                                                                                                          <  1:403491/150‑1 (MQ=255)
                             aCAGCACGGCAGGGTTGCCGTCATCGCGCTCGGCATAGGCGTTGCGGCGCAGCAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAgg                                                                                                                                            <  2:188430/131‑1 (MQ=255)
                             aCAGCACGGCAGGGTTGCCGTCATCGCGCTCGGCATAGGCGTTGCGGCGCAGCAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAgg                                                                                                                                            >  1:188430/1‑131 (MQ=255)
                                      cAGGGTTGCCGTCATCACGCCAGGCATAGGGGTTGCGGCGTAGTAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCtt                                                                                                                <  2:639971/150‑1 (MQ=255)
                                               cGTCATCGCGCTCGGCATAGGCGTTGCGTCGCAGCAAGTAGTCGAGGNCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGGGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGGTGTCTTCGACcagca                                                                                                       >  1:228795/1‑150 (MQ=255)
                                                            ggCATAGGCGTTGCGGCGCAGCAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCATGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAg                                                                                          >  1:153301/1‑150 (MQ=255)
                                                               aTAGGCGTTGCGGCGCAGCAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCat                                                                                       >  1:300907/1‑150 (MQ=255)
                                                                             gcagcaAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCATGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGAAGGTAGCGATGc                                                                         <  2:153301/150‑1 (MQ=255)
                                                                                        tCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCa                                                              >  1:611924/1‑150 (MQ=255)
                                                                                           aGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCAgtg                                                           >  2:216485/1‑150 (MQ=255)
                                                                                                 tCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGTAGGGTCAGCTCCAGGTATAGCCAGTTCACTTCTACCTCCAGAAAGCTCTTTACCATTATGGTAGCGATGCCTCAGGTAGCAGTGTTTCgg                                                     <  1:216485/150‑1 (MQ=255)
                                                                                                     cgcCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCAGTGTTTCGGGGTa                                                 <  2:790004/150‑1 (MQ=255)
                                                                                                       ccGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCatgatg                                                                                   <  1:721836/114‑1 (MQ=255)
                                                                                                       ccGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCatgatg                                                                                   >  2:721836/1‑114 (MQ=255)
                                                                                                       ccGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCAGTGTTTCGGGGTAgg                                               <  1:732404/150‑1 (MQ=255)
                                                                                                                    cGATGACCTCGTTGGCCAACTGGCTGCGCTCCAgg                                                                                                                                                     >  2:494970/1‑35 (MQ=255)
                                                                                                                    cGATGACCTCGTTGGCCAACTGGCTGCGCTCCAgg                                                                                                                                                     <  1:494970/35‑1 (MQ=255)
                                                                                                                          ccTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTa                                                                 <  2:792161/113‑1 (MQ=255)
                                                                                                                          ccTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTa                                                                 >  1:792161/1‑113 (MQ=255)
                                                                                                                           cTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCAGTGTTTCGGGGTAGGGTGAAGTGGAAGCTGGCGcc                           >  1:298900/1‑150 (MQ=255)
                                                                                                                           cTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCAGTGTTTCGGGGTAGGGTGAAGTGGAAGCTGGCGcc                           <  1:151684/150‑1 (MQ=255)
                                                                                                                            tCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCAGTGTTTCGGGGTAGGGTGAAGTGGAAGCTGGCGCCt                          >  1:388860/1‑150 (MQ=255)
                                                                                                                               ttGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCAGTGTTTCGGGGTAGGGTGAAGTGGAAGCTGgc                              <  2:198079/143‑1 (MQ=255)
                                                                                                                               ttGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCAGTGTTTCGGGGTAGGGTGAAGTGGAAGCTGgc                              >  1:198079/1‑143 (MQ=255)
                                                                                                                                    cAACTGGCTGCGCTCCATGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCAGTGTTTCGGGGTAGGGTGAAGTGGAAGCTGgc                              <  1:642517/138‑1 (MQ=255)
                                                                                                                                    cAACTGGCTGCGCTCCATGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCAGTGTTTCGGGGTAGGGTGAAGTGGAAGCTGgc                              >  2:642517/1‑138 (MQ=255)
                                                                                                                                            tGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCtt                                                                                             <  2:229671/67‑1 (MQ=255)
                                                                                                                                            tGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCtt                                                                                             >  1:229671/1‑67 (MQ=255)
                                                                                                                                                    aGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCAGTGTTTCGGGGTAGGGTGAAGTGGAAGCTGGCGCCTTGGTCGACCTGGCCAGTGGCCCAg  >  1:559748/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTCGACCTTGGGCAGTTTCAGGTCGAGTAACAGCACGGCAGGGTTGCCGTCATCGCGCTCGGCATAGGCGTTGCGGCGCAGCAAGTAGTCGAGGGCATCGGCGCCGTCACGCAGCACGATGACCTCGTTGGCCAACTGGCTGCGCTCCAGGGCCAGCAGGGTCAGCTCCAGGTCTCGGGGGTTGTCTTCGACCAGCAGAATGGGCTTGAGCATGATGGTAGCGATGCCTCAGGTAGCAGTGTTTCGGGGTAGGGTGAAGTGGAAGCTGGCGCCTTGGTCGACCTGGCCAGTGGCCCAG  >  NC_002947/2688027‑2688324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: