| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 195,634 | C→T | 45.5% | T380T (ACC→ACT) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 195,634 | 0 | C | T | 45.5% | 14.7 / 63.3 | 55 | T380T (ACC→ACT) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (17/13); new base T (15/10); total (32/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01 | |||||||||||
TTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCA > NC_002947/195490‑195782 | tttCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAAACCTCGCCCACTCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACcgc < 2:592689/150‑1 (MQ=14) cAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGAcc > 1:81570/1‑150 (MQ=255) cAGACCACGGCAAATAACGTCTACAACAAAGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGAcc > 2:628684/1‑150 (MQ=11) aGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCTACGGGCGGCAATTTCGAGCAACTGACTCCGAATCCGACCCCGGGTCAGACACCGA‑CAACAGGATCGGTCGACACCACCACCGCGAccc > 1:123878/1‑150 (MQ=11) cccGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCCAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGAGACCCTGAc > 2:6310/1‑150 (MQ=255) cGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGAACACGAACAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCTAGCCCGTc > 1:320609/1‑150 (MQ=255) gTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCg < 1:628684/150‑1 (MQ=18) cTACAACAACGGTTCGACCGTCAGCATCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTg < 2:693103/133‑1 (MQ=255) cTACAACAACGGTTCGACCGTCAGCATCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTg > 1:693103/1‑133 (MQ=255) aacaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCAcc > 1:23351/1‑150 (MQ=39) acaacGGTTCGACCGTCAGCGTCACCAACGAGAACGCCACCGCCGGTAATTCCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑AAATCGGTCGACAGCCGCACCGCGACCCTGACAGCGAGCCAGTCgagcacaa > 2:379757/1‑143 (MQ=11) gTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcg < 2:601558/150‑1 (MQ=38) gTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATATCGCGCAACAGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACGCGGTCGACACCACCACCACGGCCCTGACGGCGAGCCCGTCGCTCACCGAAggcg > 2:29321/1‑150 (MQ=11) ttCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAggcgg > 1:348121/1‑150 (MQ=12) cGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGAt < 2:81570/150‑1 (MQ=40) gCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACtt < 2:480960/150‑1 (MQ=12) aCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACAcc > 2:321561/1‑150 (MQ=17) aCCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGCCGACACCACTACCGGCACCCTGACGCCGAGCCCGTCGGCCACCACAGCCGGCGAGATCACTTAcccc > 2:110299/1‑150 (MQ=11) aTCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGAcaccaccac > 2:429237/1‑92 (MQ=255) aTCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGAcaccaccac < 1:429237/92‑1 (MQ=255) tCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGa < 2:23351/150‑1 (MQ=255) tCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGa > 1:470444/1‑150 (MQ=18) gagaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAcc < 2:420816/150‑1 (MQ=12) gaACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCt > 1:132429/1‑150 (MQ=12) aCGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGGGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa < 1:321561/150‑1 (MQ=12) cacaGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCaa > 1:228979/1‑150 (MQ=14) cACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa > 1:168989/1‑146 (MQ=255) cACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa < 2:168989/146‑1 (MQ=255) caggcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACAcc > 2:26129/3‑134 (MQ=14) caggcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACAcc < 1:26129/132‑1 (MQ=18) ttCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAc > 1:405039/1‑128 (MQ=21) ttCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAc < 2:405039/128‑1 (MQ=21) cGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGcc > 1:40570/1‑150 (MQ=25) gAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCg > 1:592078/1‑150 (MQ=18) aaCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAGGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGtga < 2:253449/150‑3 (MQ=2) aaCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCg > 2:503623/1‑83 (MQ=25) aaCTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCg < 1:503623/83‑1 (MQ=25) cccGAATTCGACCCCGGCTCAGAACATGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCCGCGTGATCACTTCCACCGTGACACTGAGCAACCCTGCCCATACGCCGGTAACCGTGaa < 2:320609/150‑1 (MQ=14) ccGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAAc > 2:346182/1‑150 (MQ=255) ccGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAAc < 2:40570/150‑1 (MQ=255) ccGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAAc > 2:756149/1‑150 (MQ=255) ccGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAAc < 1:756149/150‑1 (MQ=255) ccGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAAc > 2:254388/1‑150 (MQ=255) ccGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAAc > 1:47632/1‑150 (MQ=255) ccGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTcc > 1:163904/1‑150 (MQ=255) aCCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAAc < 2:163904/150‑1 (MQ=255) cccGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCAc < 1:331748/72‑1 (MQ=31) tCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAAc < 1:254388/150‑1 (MQ=255) aGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGACGGTCACCGACCCCGGCGTGATGCATTACACAGGAACCCTGAGTAGGCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGCCCAAACGa > 2:290139/1‑150 (MQ=11) aCCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGAtt > 2:60647/1‑150 (MQ=40) cACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTAc > 2:528237/1‑150 (MQ=255) cgatctaaC‑GACTCGGTCGACACCACCACCGCGACCCTCACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTAcc < 1:208976/144‑1 (MQ=255) aaC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCACGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGaa > 1:384137/1‑150 (MQ=255) aC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACg < 2:123572/129‑1 (MQ=255) aC‑GACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACg > 1:123572/1‑129 (MQ=255) tCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGt > 1:37054/1‑150 (MQ=40) tCGACACCACCACCGCGACCATGACGGAGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACTCCGTGACCCTTAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTaaaa < 1:29321/150‑1 (MQ=255) accactaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAg < 2:348121/150‑1 (MQ=255) ccaccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGac < 2:575836/103‑2 (MQ=255) ccaccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGac > 1:575836/1‑102 (MQ=255) caccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAg > 1:789236/1‑72 (MQ=34) caccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAg < 2:789236/72‑1 (MQ=34) accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGATAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGc > 2:636786/1‑150 (MQ=255) ccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCa < 1:6310/150‑1 (MQ=255) | TTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAAC‑GACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAGGGCA > NC_002947/195490‑195782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |