| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,002,883 | T→G | 20.0% | V294G (GTT→GGT) | PP_2626 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,002,883 | 0 | T | G | 20.0% | 30.7 / 6.7 | 20 | V294G (GTT→GGT) | PP_2626 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (7/9); new base G (2/2); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
TACCGGCTACCAGTTACGGCGTTTCGGTTTGTGGGCGCACATTCAGGTTGCTCCCCAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTATTGAAAAGAGCCTGACGGCTTGCCCCTTCTGGATTCGTGGCAGCTTTCTGGCTGCGACCGTCGCCACACAGCTGGCGATGTGCGAGGTGGCCGAAGCCATCCGTTGTGCCACCGC > NC_002947/3002740‑3003021 | tACCGGCTACCAGTTACGGCGTTTCGGTTTGTGGGCGCACATTCAGGTTGCTCCCCAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCc < 2:587507‑M2/150‑2 (MQ=255) ggCGTTTCGGTTTGTGGGCGCACATTCAGGTTGCTCCCCAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTa > 2:276471/1‑150 (MQ=255) cGGTTTGTGGGCGCACATTCAGGTTGCTCCCCAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTATTGaaaa < 1:276471/150‑1 (MQ=255) ggCGCACATTCAGGTTGCTCCCCAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTATTGAAAAGAGCCTGAc < 1:739055/150‑1 (MQ=255) aGGTTGCTCCCCAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTATTg < 1:24711/126‑1 (MQ=255) aGGTTGCTCCCCAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTATTg > 2:24711/1‑126 (MQ=255) aGGTTGCTCCCCAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTATTGAAAAGAGCCTGACGGCTTGCCCCt > 1:293024/1‑150 (MQ=255) ggTTGCTCCCCAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTATTGAAAAGAGCCTGACGGCTTGCCCCtt > 2:493057/1‑150 (MQ=255) cccAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTAtt < 1:43173/116‑1 (MQ=255) cccAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTAtt > 2:43173/1‑116 (MQ=255) ccAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGGGG‑TCAACcgccttactggcaatg < 1:74733‑M2/112‑17 (MQ=255) ccAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGGGG‑TCAACcgccttactggcaatg > 2:74733‑M2/1‑96 (MQ=255) cAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGGGG‑TCAACcgccttactggcaatg > 1:114466‑M2/1‑95 (MQ=255) cAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑GGTCAACcgccttactggcaatg < 2:114466‑M2/111‑17 (MQ=255) gggCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTATTGAAAAGAGCCTGACGGCTTGCCCCTTCTGGATTCGTGGCAGCTTTCt > 2:665561/1‑150 (MQ=255) cACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTATTGAAAAGAGCCTGACGGCTTGCCCCTTCTGGATTCGTGGCAGCTTTCTGGCTGCGACCGTCGCCACACAGCTGGCGATGTGCGAGGt < 1:493057/150‑1 (MQ=255) cgaggTTCTATGAAGAGGGCATTGCGAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCATCGTATTGAAAAGAGCCTGACTGATTGCCCCTCCTGGATTCCTGGCAGCTTTCTGGCTGCGACCTTCGCCAGACAGCTGGCGATGTGCGAGGTgg < 2:375423/147‑1 (MQ=255) gAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTATTGAAAAGAGCCTGACGGCTTGCCCCTTCTGGATTCGTGGCAGCTTTCTGGCTGCGACCGTCGc > 1:556036/1‑113 (MQ=255) gAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTATTGAAAAGAGCCTGACGGCTTGCCCCTTCTGGATTCGTGGCAGCTTTCTGGCTGCGACCGTCGc < 2:556036/113‑1 (MQ=255) cAGTAAGGCGG‑T‑TGACCccgccttgctggcaatggcctcttcatagaaccgcgtgatatccagcggcaccgccatcaattccgtacggttgccctgcctgacttggggagcaacctgaatgtgcgcccacaaaccgaaacgcagtaactg < 2:916‑M2/150‑134 (MQ=255) | TACCGGCTACCAGTTACGGCGTTTCGGTTTGTGGGCGCACATTCAGGTTGCTCCCCAAGTCAGGCAGGGCAACCGTACGGAATTGATGGCGGTGCCGCTGGATATCACGCGGTTCTATGAAGAGGCCATTGCCAGTAAGGCGG‑T‑TGACCCCGCCTTGCTGCAGCGTATTGAAAAGAGCCTGACGGCTTGCCCCTTCTGGATTCGTGGCAGCTTTCTGGCTGCGACCGTCGCCACACAGCTGGCGATGTGCGAGGTGGCCGAAGCCATCCGTTGTGCCACCGC > NC_002947/3002740‑3003021 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |