| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,021,534 | A→G | 12.5% | N677D (AAC→GAC) | PP_2638 → | cellulose synthase operon protein C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,021,534 | 0 | A | G | 12.5% | 75.2 / 4.0 | 32 | N677D (AAC→GAC) | PP_2638 | cellulose synthase operon protein C |
| Reads supporting (aligned to +/- strand): ref base A (13/15); new base G (2/2); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.09e-01 | |||||||||||
CAGCTATTACCAGCGTGTACTGGTCGTGCAGCCTGGGCGCCCTGATGCACGCCTTGGGGTGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGATTGCCGCGCAGCAGACGCGACCCGATCCGTTGTTGCGGCGCGACGCAGCCCGCCTGGTAGCGCAGGATGATCCT > NC_002947/3021386‑3021683 | cAGCTATTACCAGCGTGTACTGGTCGTGCAGCCTGGGCGCCCTGATGCACGCCTTGGGGTGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCaa > 1:662646/1‑150 (MQ=255) tGCAGCCTGGGCGCCCTGATGCACGCCTTGGGGTGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGgtgt > 1:446099/1‑150 (MQ=255) ccTGATGCACGCCTTGGGGTGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGt < 2:604030/150‑1 (MQ=255) gATGCACGCCTTGGGGTGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGaa < 2:446099/150‑1 (MQ=255) aTGCACGCCTTGGGGTGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAac < 1:471760/150‑1 (MQ=255) gCCTTGGGGTGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATc > 1:24702/1‑150 (MQ=255) gCCTTGGGGTGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATc > 1:661597/1‑150 (MQ=255) cTTGGGGTGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAg > 1:124766/1‑150 (MQ=255) gggTGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGcggcgg > 2:377637/1‑117 (MQ=255) gggTGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGcggcgg < 1:377637/117‑1 (MQ=255) ggTGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAggcggc > 2:582407/1‑150 (MQ=255) tGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCGACGCCCAGCGGCGCCTgg > 2:664555/1‑108 (MQ=255) tGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCGACGCCCAGCGGCGCCTgg > 2:664570/1‑108 (MQ=255) tGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCGACGCCCAGCGGCGCCTgg < 1:664555/108‑1 (MQ=255) tGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCGACGCCCAGCGGCGCCTgg < 1:664570/108‑1 (MQ=255) aTGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGATCGCCACTTCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGAGAAAACGATCAGGCGGCCAgg > 1:251053/1‑150 (MQ=255) cGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCg < 2:662646/150‑1 (MQ=255) ggCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGccc > 2:794849/1‑80 (MQ=255) ggCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGccc < 1:794849/80‑1 (MQ=255) ccGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGa > 1:207605/1‑147 (MQ=255) ccGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGa < 2:207605/147‑1 (MQ=255) aGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGATTGCcgc < 1:85143/150‑1 (MQ=255) aaCCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGATTGCCGCgcag < 2:756496/150‑1 (MQ=255) cagGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGATTGcc < 2:280734/135‑1 (MQ=255) cagGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGATTGcc > 1:280734/1‑135 (MQ=255) ggCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGtgtg < 2:477942/81‑1 (MQ=255) ggCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGtgtg > 1:477942/1‑81 (MQ=255) tGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGATTGCCGCGCAGCAGACGCTACCCGATCCgttgt < 2:251053/150‑1 (MQ=255) tGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGATTGCCGCGCAGCAGACGCGACCCGATCCgttgt < 2:661597/150‑1 (MQ=255) gCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGATTGCCGCGCAGCAGACGCGACCCGATCCGTTGTTgcggc < 2:124766/150‑1 (MQ=255) gCCACAATTTGCCGCTGATGACGGAAATGCTCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGATTGCCGCGCAGCAGACGCGACCCGATCCGTTGTTGCGGCGCGACGCAGc < 1:483/150‑1 (MQ=255) ccGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGATTGCCGCGCAGCAGACGCGACCCGATCCGTTGTTGCGGCGCGACGCAGCCCGCCTGGTAg > 1:303503/1‑150 (MQ=255) ggCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGATTGCCGCGCAGCAGACGCGACCCGATCCGTTGTTGCGGCGCGATGCAGCCCGCCTGGTAGCGCAGgatgat > 1:294944/1‑150 (MQ=255) aaCGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGAGGCCAGGCTGCTTGACCGGATTGCCGCGCAGCAGACGCGACCCGATCCGTTGTTGCGGCGCGACGCAGCCCGCCTGGTAGCGCAGGATGATCCt > 2:362937/1‑150 (MQ=255) | CAGCTATTACCAGCGTGTACTGGTCGTGCAGCCTGGGCGCCCTGATGCACGCCTTGGGGTGATGGAAAGCGCCGTGGCCGAAGGCAACCTGCAGCAGGCCCGGCACATGCTGGGCGTGAAGGTGCCACAATTTGCCGCTGATGACGGCAACGCCCAGCGGCGCCTGGCGGCGGTGTGGACCGCGCTGGGTGAACACGATCAGGCGGCCAGGCTGCTTGACCGGATTGCCGCGCAGCAGACGCGACCCGATCCGTTGTTGCGGCGCGACGCAGCCCGCCTGGTAGCGCAGGATGATCCT > NC_002947/3021386‑3021683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |