| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,104,342 | T→A | 17.2% | L276Q (CTG→CAG) | PP_2714 → | sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,104,342 | 0 | T | A | 17.2% | 69.4 / 12.3 | 35 | L276Q (CTG→CAG) | PP_2714 | sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base T (16/13); new base A (4/2); total (20/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-02 | |||||||||||
TTGCTGGCGCAGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTGGCTGAACTGACC > NC_002947/3104194‑3104490 | ttgatGGCGCAGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTg < 2:660441/146‑1 (MQ=255) tGCTGGCGCAGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTgg < 1:555241/150‑1 (MQ=255) gcAGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCt > 2:774367/1‑150 (MQ=255) aTCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTtgtg < 2:448246/150‑1 (MQ=255) cgacgaTCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTggg > 2:730219/1‑150 (MQ=255) ctgcGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGAt < 1:338860/150‑1 (MQ=255) cTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCACAGAACGCGCTGGCCGCGAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGCAAGGggtggc > 1:346586/1‑149 (MQ=255) ttGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAAcc > 1:458969/1‑150 (MQ=255) gATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTg > 1:39893/1‑150 (MQ=255) gcCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGAcc < 1:605823/150‑1 (MQ=255) ccACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCACTAGTGAGACTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCtt > 1:607123/1‑150 (MQ=255) ccACGAAATACGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCtt > 1:468322/1‑150 (MQ=255) cACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTg > 2:304422/1‑150 (MQ=255) aCGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCAAGAAAGCCCAGGGCTTTCtg > 2:744990/1‑97 (MQ=255) aCGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCAAGAAAGCCCAGGGCTTTCtg < 1:744990/98‑2 (MQ=255) aCGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAg < 2:39893/150‑1 (MQ=255) ctctGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCAGACCTTGGCCCGCGTCGAGCCaa < 1:304422/150‑1 (MQ=255) gCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTg > 2:416794/1‑150 (MQ=255) gcCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTggg > 2:562652/1‑142 (MQ=255) gcCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTggg < 1:562652/142‑1 (MQ=255) gcCTGCACGCGCAGAACGCACTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCATCGAGCCAAGACTGGGGCAGCGAg > 2:628395/1‑150 (MQ=255) gcgcTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGAc > 2:362/1‑150 (MQ=255) ccAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCCTAATCTTGCAGCCCCTGGCTTTCTTCAAGCCAATACTGGGGGAGCGAGCCAGCACCCGGATCGACCTGGCCGAgg < 1:216597/150‑1 (MQ=255) cAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGgt > 1:445833/1‑150 (MQ=255) cAATGATGCCGAGCGCCAGAAAGCCCTGGGTTTTCTTGTGGGCGGGGTCGATCGGCTGATACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGgtcgt < 1:730219/150‑1 (MQ=255) cAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTGGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTGGCTGACCTTGGCCCGCGTCGAGCCCAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGgtcgt < 2:317990/150‑1 (MQ=255) tgatgCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCAc < 1:362/150‑1 (MQ=255) cGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAAc > 1:224983/1‑150 (MQ=255) cacaaGAAAGCCCAGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTg > 2:246369/5‑150 (MQ=255) gcCAGAAAGCCCTGGGCCTTCTTCTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCAAGCGTCGAGCCAAGCCTGGGGAGGAGGGCCACCACCCGGATCGACCTCACCTAAGTCGTCACCTAAACCCTgg < 2:607123/150‑1 (MQ=255) acaaGAAAGCCCAGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTg < 1:15723/122‑1 (MQ=255) acaaGAAAGCCCAGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTg > 2:15723/4‑125 (MQ=255) acaaGAAAGCCCAGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTgg > 1:629720/4‑150 (MQ=255) cccTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTGGCTCAACtta > 1:118752/1‑148 (MQ=255) cTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGTGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTGGCTGAACTGAcc > 1:386169/1‑150 (MQ=255) | TTGCTGGCGCAGATCGACGATCTGCTGCGGCGTGAACATCGCTTCATTGCCGATGCTGCCCACGAAATGCGTACGCCTCTGGCCGTATTGCGCCTGCACGCGCAGAACGCGCTGGCCGCCAGCAATGATGCCGAGCGCCAGAAAGCCCTGGGCTTTCTTGTGGGCGGGGTCGATCGGCTGACACGGGTGGTCAACCAGTTGCTGACCTTGGCCCGCGTCGAGCCAAGACTGGGGCAGCGAGCCAGCACCCGGATCGACCTGGCCGAGGTCGTCACCGAAACCCTGGCTGAACTGACC > NC_002947/3104194‑3104490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |