Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,166,099 G→C 14.6% G163A (GGC→GCC)  PP_2780 → 3‑oxoacyl‑ACP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,166,0990GC14.6% 119.0 / 15.4 48G163A (GGC→GCC) PP_27803‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand):  ref base G (19/22);  new base C (3/4);  total (22/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.01e-01

AGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTG  >  NC_002947/3165961‑3166241
                                                                                                                                          |                                                                                                                                              
aGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGACTGCCGATGCCATCGGCaatc                                                                                                                                     <  2:601810/150‑4 (MQ=255)
 ggACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCCGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGCCATCGGCaatc                                                                                                                                     <  2:317731/149‑4 (MQ=255)
 ggACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCCGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGCCATCGGCaatc                                                                                                                                     >  1:317731/1‑146 (MQ=255)
  gACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGaa                                                                                                                                   <  2:711732/150‑1 (MQ=255)
       gctgctCAGCCTGGCGGATAGCCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCTTCAGAGTAACGACCGCCTTGCGCTGGTGATTGCCGATGGCATCGGCAGTGAAGGccc                                                                                                                              <  2:28575/150‑1 (MQ=255)
         tgctCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCgg                                                                                                                            <  1:374663/150‑1 (MQ=255)
                  tGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATc                                                                                                                       >  1:28775/1‑146 (MQ=255)
                  tGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATc                                                                                                                       <  2:28775/146‑1 (MQ=255)
                     cGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTca                                                                                                                <  1:53373/150‑1 (MQ=255)
                            cccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAgcgc                                                                                                         >  2:183166/1‑150 (MQ=255)
                            cccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAgcgc                                                                                                         >  1:597360/1‑150 (MQ=255)
                             ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGaa                                                                                                                                   <  2:317329/123‑1 (MQ=255)
                             ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGaa                                                                                                                                   >  1:317329/1‑123 (MQ=255)
                             ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCagcagc                                                                                                           <  2:745521/147‑1 (MQ=255)
                             ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCagcagc                                                                                                           >  1:745521/1‑147 (MQ=255)
                             ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGcc                                                                                                        >  1:651814/1‑150 (MQ=255)
                             ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGcc                                                                                                        >  1:392910/1‑150 (MQ=255)
                             ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGGTCACTGCGTCAGAGGAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCACGGTGATGAATGTCAGCAGCGcc                                                                                                        >  2:36203/1‑150 (MQ=255)
                                                    tCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGt                                                                                                                           >  2:25974/1‑108 (MQ=255)
                                                    tCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGt                                                                                                                           <  1:25974/108‑1 (MQ=255)
                                                    tCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCAt                                                                                 >  2:437841/1‑150 (MQ=255)
                                                      aaTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTcc                                                                                         >  2:427697/1‑140 (MQ=255)
                                                      aaTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTcc                                                                                         <  1:427697/140‑1 (MQ=255)
                                                      aaTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCg                                                                               >  1:457768/1‑150 (MQ=255)
                                                                gcgACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTca                                                                                                                >  1:456086/1‑107 (MQ=255)
                                                                gcgACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTca                                                                                                                <  2:456086/107‑1 (MQ=255)
                                                                     aGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAga                                                                <  2:597360/150‑1 (MQ=255)
                                                                      ggCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAgat                                                               <  1:582569/150‑1 (MQ=255)
                                                                                 aTCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTcgcg                                                    >  2:507049/1‑150 (MQ=255)
                                                                                    cTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGGGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCgcggcg                                                 >  2:575141/1‑150 (MQ=255)
                                                                                        ccgatctcTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGACAAACCGTCGCGGCGAGGt                                             <  1:625910/143‑1 (MQ=255)
                                                                                                tGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATAAATAAAAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCACTGCATTCCAGATGATCCGTCGCGGCGAGGTcagatcgg                                     >  2:625910/1‑143 (MQ=255)
                                                                                                    tCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCAt                                 >  1:351293/1‑150 (MQ=255)
                                                                                                      agagCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTg                               <  2:457768/150‑1 (MQ=255)
                                                                                                          cagcGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCg                                                         >  1:798256/4‑120 (MQ=255)
                                                                                                          cAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCg                                                         <  2:798256/120‑1 (MQ=255)
                                                                                                          cAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCgg                           <  2:651814/150‑1 (MQ=255)
                                                                                                                ccGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGgcgc                     <  2:119330/150‑1 (MQ=255)
                                                                                                                       gcgcGGGTCATTGGGCTTGGGATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCg              <  2:222360/150‑1 (MQ=255)
                                                                                                                         gcgGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGt            <  2:108886/150‑1 (MQ=255)
                                                                                                                              tGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTa       <  2:351293/150‑1 (MQ=255)
                                                                                                                                actGCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGc     >  1:346968/3‑150 (MQ=255)
                                                                                                                                aTTGCCGATGGCATCGGAAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGc     <  2:427001/150‑1 (MQ=255)
                                                                                                                                   gCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTg  <  2:346968/150‑1 (MQ=255)
                                                                                                                                    ccGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCg                                                                               >  2:141737/1‑72 (MQ=255)
                                                                                                                                    ccGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCg                                                                               <  1:141737/72‑1 (MQ=255)
                                                                                                                                     cGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCAt                                 >  2:162578/1‑117 (MQ=255)
                                                                                                                                     cGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCAt                                 <  1:162578/117‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                              
AGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTG  >  NC_002947/3165961‑3166241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑