| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,253,091 | C→G | 18.2% | G132R (GGC→CGC) | PP_2851 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,253,091 | 0 | C | G | 18.2% | 78.3 / 13.1 | 33 | G132R (GGC→CGC) | PP_2851 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (14/13); new base G (3/3); total (17/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 | |||||||||||
GGAAAAAAGCACTCAAGCCGGGCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGCTCCAGCCTTCGG > NC_002947/3252944‑3253240 | ggAAAAAAGCACTCAAGCCGGGCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCa < 2:91673/150‑1 (MQ=255) cTCAAGCCGGGCAGCTCGCTTTCATTGCCCTCATATGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACCGCCAGCGCATGGGCTGGcag < 1:479459/150‑3 (MQ=255) cTCAAGCCGGGCAGCTCGCTTTCATTGCCCTCATATGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACCGCCAGCGCATGGGCTGGcag > 2:479459/1‑148 (MQ=255) cTCAAGCCGGGCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCGCATGGGCTGGcag < 2:498071/150‑3 (MQ=255) aaGCCGGGCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCCACGAATAACACGGGAACTGCCAGCCCATGCGCTGGCGAAAt < 1:447565/150‑1 (MQ=255) gggCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTg < 2:136913/150‑1 (MQ=255) gggCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCACTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTg < 2:106005/150‑1 (MQ=255) cTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTc > 2:507570/1‑150 (MQ=255) cGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCGCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCgg < 2:254500/150‑1 (MQ=255) aTTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACgg > 1:75157/1‑150 (MQ=255) ccTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGccc > 1:98092/1‑150 (MQ=255) ccTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGccc < 1:674380/150‑1 (MQ=255) ccTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGccc < 2:75157/150‑1 (MQ=255) ccTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGccc > 2:674380/1‑150 (MQ=255) ccTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGccc > 1:457120/1‑150 (MQ=255) tCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGt > 2:353689/1‑150 (MQ=255) aTAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGccac > 2:526169/1‑150 (MQ=255) cTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCGCATGGGCTGGcagt > 1:324491/1‑98 (MQ=255) cTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCGCATGGGCTGGcagt < 2:324491/101‑4 (MQ=255) ttCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCa > 1:766389/1‑106 (MQ=255) ttCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCa < 2:766389/106‑1 (MQ=255) ttCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGc > 2:796791/1‑150 (MQ=255) cGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCa < 1:507570/150‑1 (MQ=255) tGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGt > 1:788134/1‑150 (MQ=255) ccGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGc < 2:457120/150‑1 (MQ=255) acacTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGa > 2:246249/1‑150 (MQ=255) acacTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGa < 2:788134/150‑1 (MQ=255) tGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCgtggtg > 1:166177/1‑133 (MQ=255) tGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCgtggtg < 2:166177/133‑1 (MQ=255) tCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTgg > 2:650177/1‑150 (MQ=255) ttcgCCAGCGCATGGGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGCTcc > 2:322574/4‑150 (MQ=255) gCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCATATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGc < 2:754517/144‑1 (MQ=255) gCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCATATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGc > 1:754517/1‑144 (MQ=255) ccATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGCTCCAGCCTTCgg > 1:629362/1‑150 (MQ=255) | GGAAAAAAGCACTCAAGCCGGGCAGCTCGCTTTCATTGCCCTCATACGGCTCATAGTTGTACTGCCGCTGGCCTTCGCTGCCGACACTGACGCCAAAGTCCTCGTTGAAGTCGCTGCCATGCGACGAATACCACGGGAACTGCCAGCCCATGCGCTGGCGAAATGCCTGGAATTCGGCATACGGCGCCCGTGACACCGCCACCAGCGACACATCGTGGTGGGCCAGGTGCAGATTGGCGCCGTCATAGTGGTCGGCCAGAAAAGAGCAGCCGGGGCAGCCCTCGCTCCAGCCTTCGG > NC_002947/3252944‑3253240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |