| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,295,574 | T→G | 11.8% | K52T (AAG→ACG) ‡ | PP_2895 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,295,574 | 0 | T | G | 11.8% | 76.6 / 5.7 | 34 | K52T (AAG→ACG) ‡ | PP_2895 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (14/16); new base G (2/2); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.56e-01 | |||||||||||
TACAGTGCCCTCTTCGATGGAAATGTTCGGGTGGGTAATGGCCGCCATCAGCGGCACTTTCATGGTCTTGCTGACCACGCCTTTGTATTCGCCCTGTTCGTCGACAATGGTTTCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGCGGTCCATCGGCAGGGCATTGATCACGGAGCCTATAAGGCTGTTATCTAA > NC_002947/3295432‑3295722 | tACAGTACCCTCTTCGATGGAAATGTTCGGGTGGGTAATGGCCGCCATCAGCGGCACTTTCATGGTCTTGCTGACCACGCCTTTGTATTCGCCCTGTTCGTCGACAATGGTTTCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATc > 2:294620/1‑150 (MQ=255) gCCCTCTTCGATGGAAATGTTCGGGTGGGTAATGGCCGCCATCAGCGGCACTTTCATGGTCTTGCTGACCACGCCTTTGTATTCGCCCTGTTCGTCGACAATGGTTTCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGAc < 2:428036/150‑1 (MQ=255) ctTCGATGGAAATGTTCGGGTGGGTAATGGCCGCCATCAGCGGCACTTTCATGGTCTTGCTGACCACGCCTTTGTATTCGCCCTGTTCGTCGACAATGGTTTCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATa < 2:604506/150‑1 (MQ=255) cGATGGAAATGTTCGGGTGGGTAATGGCCGCCATCAGCGGCACTTTCATGGTCTTGCTGACCACGCCTTTGTATTCGCCCTGTTCGTCGACAATGGTTTCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑ACATCCTGGACGCATACCt < 2:610099/150‑1 (MQ=255) gtgggtAATGGCCGCCATCAGCGGCACTTTCATGGTCTTGCTGACCACGCCTTTGTATTCGCCCTGTTCGTCGACAATGGTTTCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGc < 2:81398/150‑1 (MQ=255) gtgggtAATGGCCGCCATCAGCGGCACTTTCATGGTCTTGCTGACCACGCCTTTGTATTCGCCCTGTTCGTCGACAATGGTTTCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGc < 1:440817/150‑1 (MQ=255) tCTTGCTGACCACGCCTTTGTATTCGCCCTGTTCGTCGACAATGGTTTCGTCGTAGTCGAACTGGATGGCCACTGGCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCa > 2:61914/1‑150 (MQ=255) cACGCGTTTGTATTAGCCCTGTTCGTCGGTAATGGTTTCGTCGTAGTCGAACGGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCaa < 2:565530/150‑1 (MQ=255) gCCTTTGTATTCGCCCTGTTCGTCGACAATGGTTTCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAggg > 2:337735/1‑150 (MQ=255) tGTATTCGCCCTGTTCGTCGACAATGGTTTCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCgg < 1:294620/150‑1 (MQ=255) tcgACAATGGTTTCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAg > 1:539617/1‑126 (MQ=255) tcgACAATGGTTTCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAg < 2:539617/126‑1 (MQ=255) ctaCAATGGTTTCGTCGTAGTCGAACTGGATAGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGCCAGCAAGCTCCTGTACCATGGCCTGCAAGGGGCCGGCGATCATGCGGTCCATCg < 1:337735/148‑1 (MQ=255) ctgatgcaggtatgcgtccaGGATGGCAAG‑GCAGTGGCCATCCAGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGc > 1:59724‑M1/21‑126 (MQ=255) ctgatgcaggtatgcgtccaGGATGGCAAG‑GCAGTGGCCATCCAGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGc < 2:59724‑M1/106‑1 (MQ=255) gTTTCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGCGGTCCATCGGCAGGGCa > 1:559371/1‑150 (MQ=255) tttCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGCTTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGCGGt < 2:163420/135‑1 (MQ=255) tttCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGCGGt > 1:163420/1‑135 (MQ=255) gatgcaggtatgcgtccaGGATGGCAAG‑GCAGTGGCCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCaa < 2:519965‑M1/97‑1 (MQ=255) gatgcaggtatgcgtccaGGATGGCAAG‑GCAGTGGCCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCaa > 1:519965‑M1/19‑115 (MQ=255) gtcgtAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTg < 1:98156/90‑1 (MQ=255) gtcgtAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTg > 2:98156/1‑90 (MQ=255) gTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCAc < 1:750185/93‑1 (MQ=255) gTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCAc > 2:750185/1‑93 (MQ=255) GGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGCGGTCCATCGGCa < 2:515579‑M1/126‑1 (MQ=255) GGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGCGGTCCATCGGCa > 1:515579‑M1/1‑126 (MQ=255) GGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGCGGTCCATCGGCAGGGCATTGATCACGGAGCCTATAAGGCt > 2:559371‑M1/1‑150 (MQ=255) CCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGCGGTCCATCGGCAGGGCATTGATCACGGAGCCTATAAGGCTGt < 1:389237‑M1/150‑1 (MQ=255) cACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGc > 1:707699/1‑107 (MQ=255) cACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGc < 2:707699/107‑1 (MQ=255) cACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAACCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCCGCGATCATGCGGTCCATCGGCAGGGCATTGATCACGGAGCCTATAAGGCTGtt > 1:759958/1‑150 (MQ=255) tGCCTCG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGCGGTCCATCGGCAGGGCATTGATCACGGAGCCta > 2:107950/1‑137 (MQ=255) tGCCTCG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGCGGTCCATCGGCAGGGCATTGATCACGGAGCCta < 1:107950/137‑1 (MQ=255) cTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGCGGTCCATCGGCAGGGCATTGATCACGGAGCCTATAAGGCTGTTATCTaa > 1:80154/1‑150 (MQ=255) | TACAGTGCCCTCTTCGATGGAAATGTTCGGGTGGGTAATGGCCGCCATCAGCGGCACTTTCATGGTCTTGCTGACCACGCCTTTGTATTCGCCCTGTTCGTCGACAATGGTTTCGTCGTAGTCGAACTGGATGGCCACTGCCTTG‑CCATCCTGGACGCATACCTGCATCAGGAAATCGGCGTACGATTTGCTGGCGGTTACCTGCGCCTGCACCATGGCCTGCAAGGGGCCGGCGATCATGCGGTCCATCGGCAGGGCATTGATCACGGAGCCTATAAGGCTGTTATCTAA > NC_002947/3295432‑3295722 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |