| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,310,151 | T→G | 16.1% | L408R (CTG→CGG) ‡ | lhgO → | L‑2‑hydroxyglutarate oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,310,151 | 0 | T | G | 16.1% | 48.9 / 5.6 | 25 | L408R (CTG→CGG) ‡ | lhgO | L‑2‑hydroxyglutarate oxidase |
| Reads supporting (aligned to +/- strand): ref base T (12/9); new base G (2/2); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01 | |||||||||||
TCGTCAACACCGCACGCAGTGTCAACGTGTGCAACGCACCGTCGCCAGCTGCTACCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCTTCTGCGGTTTCGTTTTACTCAACCCGTACTGCCCTGCTCACCATTCAGGGCAGGGCGGGCTTTTTCGCGCCTGCGTCGGTGACGTGCAGCCGGCGGC > NC_002947/3310010‑3310292 | tCGTCAACACCGCACGCAGTGTCAACGTGTGCAACGCACCGTCGCCAGCTGCTACCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACAGGTCGGcct < 1:231573/150‑2 (MQ=255) gTCAACACCGCACGCAGTGTCAACGTGTGCAACGCACCGTCGCCAGCTGCTACCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACAGGTCGGcct > 2:76724/1‑147 (MQ=255) gTCAACACCGCACGCAGTGTCAACGTGTGCAACGCACCGTCGCCAGCTGCTACCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACAGGTCGGcct < 1:76724/148‑2 (MQ=255) tCAACACCGCACGCAGTGTCAACGTGTGCAACGCACCGTCGCCAGCTGCTACCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAAcc > 1:417068/1‑150 (MQ=255) tCAACACCGCACGCAGTGTCAACGTGTGCAACGCACCGTCGCCAGCTGCTACCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAAc < 2:1000/149‑1 (MQ=255) tCAACACCGCACGCAGTGTCAACGTGTGCAACGCACCGTCGCCAGCTGCTACCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAAc > 1:1000/1‑149 (MQ=255) aCGCACCGTCGCCAGCTGCTACCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAaca > 2:730579/1‑150 (MQ=255) gCACCGTCGCCAGCTGCTACCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAg < 2:731019/131‑1 (MQ=255) gCACCGTCGCCAGCTGCTACCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGACGACCTGTCGGCCAACCAGAGGGCTGCCAg > 1:731019/1‑131 (MQ=255) tCGCCAGCTGCTACCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCTTCTGc > 2:105664/1‑150 (MQ=255) ccAGCTGCTACCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCTTCTGCGGt > 1:434772/1‑150 (MQ=255) aCCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCTTCTGCGGTTTCGTTTTa > 1:566277/1‑150 (MQ=255) ccGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGTGCTGTCATATGACAGCCGGGTAACACCTTCTGCGGTTTCGTTTTACTCAACCCGTAc < 2:195446/150‑1 (MQ=255) cGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGCCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCTTCTGCGGTTTCGTTTTACTCAACCCGTACt > 2:359564/1‑150 (MQ=255) gcgcCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAac < 1:618289/110‑1 (MQ=255) gcgcCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAac > 2:618289/1‑110 (MQ=255) gtgCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCTTCTGCGGTTTCGTTTTACTCAACCCGTACTGCCCTGCTCACCATTCAGGGCAgggcgg > 2:54451/1‑150 (MQ=255) gAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGAACTATAGGCAAATCAGAGGGCTGCCAGCTGACAGACGGGTAACACCTTCTGCGGTTTCGTTTTACTCAACCCGTACTGCCCTGCTCACCATTCAGGGCAGGGCGGGCtt < 1:776613/150‑1 (MQ=255) tCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCTTCTGCGGTTTCGTTTTACTCAACCCGTACTGCCCTGCTCACCATTCAGGGCAGGGCGGGCTTTTTcgcg > 1:614199/1‑150 (MQ=255) cGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCTTCTGCGGTTTCGTTTTACTCAACCCGTACTGCCCTGCTCACCATTCAGGGCAGGGCGGGCTTTTTCgcgc < 1:140762/150‑1 (MQ=255) aGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCtt < 2:171780/73‑1 (MQ=255) aGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCtt > 1:171780/1‑73 (MQ=255) cTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCTTCTGCGGTTTCGTTTTACTCAACCCGTACTGCCCTGCTCACCATTCAGGGCAGGGCGGGCTTTTTCGCGCCTGCGTCGGTGACGTGCa < 2:742708/150‑1 (MQ=255) gttggCCGACAGGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCTTCTGCGGTTTCGTTTTACTCAACCCGTACTGCCCTGCTCACCATTCAGGGCAGGGCGGGCTTTTTCGCGCCTGCGTCGGTGACGTGCAGCcggc > 2:561890/4‑150 (MQ=255) ggCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCTTCTGCGGTTTCGTTTTACTCAACCCGTACTGCCCTGCTCACCATTCAGGGCAGGGCGGGCTTTTTCGCGCCTGCGTCGGTGACGTGCAGCCggcggc < 2:566277/150‑1 (MQ=255) | TCGTCAACACCGCACGCAGTGTCAACGTGTGCAACGCACCGTCGCCAGCTGCTACCTCGGCCATCCCGATCGGCGCCTACATCGTCGAGAAGGTGTGCGAGCAGGTCGGCCTGCAGGGCGGCAGCTTCCCCAAGGCCGACCTGTCGGCCAACCAGAGGGCTGCCAGCTGACAGCCGGGTAACACCTTCTGCGGTTTCGTTTTACTCAACCCGTACTGCCCTGCTCACCATTCAGGGCAGGGCGGGCTTTTTCGCGCCTGCGTCGGTGACGTGCAGCCGGCGGC > NC_002947/3310010‑3310292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |