| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,315,630 | G→T | 20.6% | A191E (GCG→GAG) | PP_2915 ← | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,315,630 | 0 | G | T | 20.6% | 75.1 / 11.1 | 34 | A191E (GCG→GAG) | PP_2915 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (16/11); new base T (2/5); total (18/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
ACCCGCGCATCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCAC > NC_002947/3315483‑3315775 | aCCCGCGCATCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGcgcg > 2:763367/1‑150 (MQ=255) cccGCGCATCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGACTGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCgcgg < 1:763367/150‑1 (MQ=255) ccGCGCATCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGCGCtcggc < 1:200419/150‑1 (MQ=255) cATCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGAGATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGCGCTCGGCGcctg < 1:350646/150‑3 (MQ=255) aTCGAATGATCCGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGCGCTCGGCGcctgg < 1:745333/150‑4 (MQ=255) aTCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGTGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAg < 2:592393/150‑1 (MQ=255) aTCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAg > 2:756632/1‑150 (MQ=255) cGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCGGGCGCCGCGCTCGGCGcctggtt < 1:374991/150‑6 (MQ=255) ggCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCaa < 1:756632/150‑1 (MQ=255) gCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGCGCTCGGCGcctggtt > 2:759205/1‑123 (MQ=255) gCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGCGCTCGGCGcctggtt < 1:759205/128‑6 (MQ=255) gCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCaa > 2:254691/1‑150 (MQ=255) aGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGcc > 2:439099/1‑150 (MQ=255) ggACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCa > 2:770572/1‑150 (MQ=255) ccGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGcc > 1:470678/1‑150 (MQ=255) cgcgGCAGGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGCCCCAAGACACCAACTGCACCCGCCAGCCg > 2:211616/1‑150 (MQ=255) aaCCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGcgccgc < 1:527624/150‑1 (MQ=255) cgcgATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGCGCTCGGCGcctgg > 2:657520/1‑95 (MQ=255) cgcgATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGCGCTCGGCGcctgg < 1:657520/98‑4 (MQ=255) tCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCcagca > 1:260767/1‑150 (MQ=255) aGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTCGCGCCGCTCGCCCagcagc < 1:254691/150‑1 (MQ=255) ccAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAAc > 1:603043/1‑150 (MQ=255) cGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCa < 2:473454/150‑1 (MQ=255) gCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAg > 1:326664/1‑150 (MQ=255) ccAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCcgc < 2:470678/150‑1 (MQ=255) gCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAAcg < 2:16197/150‑1 (MQ=255) gcgcCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGgcgc > 2:737636/1‑150 (MQ=255) gcCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGccc < 2:326664/150‑1 (MQ=255) ccAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTaa > 2:287061/1‑149 (MQ=255) ccAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAg > 1:637981/1‑150 (MQ=255) ccAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCACAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTaa < 1:287061/149‑1 (MQ=255) gtgtTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAg > 2:196307/1‑150 (MQ=255) gttgCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGgc > 2:63864/1‑127 (MQ=255) gttgCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGgc < 1:63864/127‑1 (MQ=255) gttgCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGa > 1:173232/1‑150 (MQ=255) cGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCa < 1:737636/150‑1 (MQ=255) gAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCAc > 2:728218/1‑150 (MQ=255) | ACCCGCGCATCGAATGATCAGCGCGGCATGTAGCCACGCTGCCAGGACCGCGGAATGAACCGCGATATCAGCGCCAGCACGCAGGCCAGCGCACAGCTGCCGGCCAGCGCCTGCAGGCCCAGGTGTTGTTGCAACCAGGCGCCGAGCGCGGCGCCCAGCAACATGCCGGCCCAAGGCACCAACTGCACCCGCCAGCCGTTGCGCCGCTCGCCCAGCAGCCAACGCCCCAGGCCGCGGCCGAAACGCGACAGGGCGCCCGTTACGTAAGTCAGGCCGATGGGCAGGCCATTCAC > NC_002947/3315483‑3315775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |