| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,326,898 | G→T | 12.9% | V536V (GTG→GTT) | mqo‑III → | malate:quinone oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,326,898 | 0 | G | T | 12.9% | 79.9 / 3.8 | 32 | V536V (GTG→GTT) | mqo‑III | malate:quinone oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (12/15); new base T (2/2); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01 | |||||||||||
CAGATCATCCCGTCCTATGGCATCCGCCTGAACGACCACCCGGATCAGCTAGAGAAGGAGTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCATGTGCTCGGGTTCGTACAGGTTGCGGCCATCGAACACCACCCGGTCGGC > NC_002947/3326749‑3327027 | cAGATCATCCCGTCCTATGGCATCCGCCTGAACGACCACCCGGATCAGCTAGAGAAGGAGTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTg > 2:390163/1‑150 (MQ=255) atcCCGTCCTATGGCATCCGCCTGAACGACCACCCGGATCAGCTAGAGAAGGAGTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGc > 2:27581/1‑150 (MQ=255) tATGGCATCCGCCTGAACGACCACCCGGATCAGCTAGAGCAGCTGCTGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCa > 2:425770/1‑150 (MQ=255) gCCTGAACGACCACCCGGATCAGCTAGAGAAGGAGTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCaa > 1:715829/1‑150 (MQ=255) cGACCACCCGGATCAGCTAGAGAAGGAGTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGgcg < 1:390163/150‑1 (MQ=255) accCGGATCAGCTAGAGAAGGAGTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTTGCCAGCCGGTAATCATTGcc > 1:270201/1‑133 (MQ=255) accCGGATCAGCTAGAGAAGGAGTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTTGCCAGCCGGTAATCATTGcc < 2:270201/133‑1 (MQ=255) ccGGATCAGCTAGAGAAGGAGTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCtt > 2:558989/1‑150 (MQ=255) ggATCAGCTAGAGAAGGAGTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTgg < 1:27581/150‑2 (MQ=255) agaAGGAGTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATg > 1:705077/1‑150 (MQ=255) ggAGTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCAGGATGTGGATGGCGGTGTCGTGTTCGGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCgt > 1:181408/1‑150 (MQ=255) gTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAAt > 2:726145/1‑127 (MQ=255) gTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAAt < 1:726145/127‑1 (MQ=255) atatACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAagcca < 2:738645/150‑1 (MQ=255) cAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTg > 2:196582/1‑150 (MQ=255) aaCGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAagccagcgcaag < 1:425770/150‑6 (MQ=255) aGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAg < 2:745848/150‑1 (MQ=255) gctgcAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGc > 1:288032/1‑72 (MQ=255) gctgcAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGc < 2:288032/72‑1 (MQ=255) tgcAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCAtg > 1:628020/1‑150 (MQ=255) tGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCATGTGCTCgg < 2:715829/150‑1 (MQ=255) tGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCATGTGCTCgg < 2:451434/150‑1 (MQ=255) gAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCATGTGCTCggg < 1:57353/150‑1 (MQ=255) tCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTTGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCATGTGCTCGGGTTCGTACAgg > 1:195166/1‑150 (MQ=255) tCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGa < 2:281660/121‑1 (MQ=255) tCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGa > 1:281660/1‑121 (MQ=255) gCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCATGTGCTCGGGTTCGTACAGGTTGCGGc < 2:628020/150‑1 (MQ=255) aGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCATGTGCTCGGGTTCGTACAGGTTGCGGCCATCGAAcacc < 2:181408/150‑1 (MQ=255) tCGATAAGGCCGGCTGACAGCCAGGGAGTTGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCATGTGCTCGGGTTCGTACAGGTTGCGGCCATCGAAcaccac < 2:195166/150‑1 (MQ=255) gCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCATGTGCTCGGGTTCGTAAAATCTGCGGCCATCGAACACCACCCGGTCgg > 2:582240/1‑150 (MQ=255) ccGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCATGTGCTCGGGTTCGTACAGGTTGCGGCCATCGAACACCACCCGGTCGGc < 1:558989/150‑1 (MQ=255) ccAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCATGTGCTCGGGTTCGTACAGGTTGCGGCCATCGaa > 2:591582/1‑124 (MQ=255) ccAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCATGTGCTCGGGTTCGTACAGGTTGCGGCCATCGaa < 1:591582/124‑1 (MQ=255) | CAGATCATCCCGTCCTATGGCATCCGCCTGAACGACCACCCGGATCAGCTAGAGAAGGAGTGGGCATATACCAACGAGGTGCTGCAGCTTGAGCCGCCTGTCAGTCCGCAGCGCCCTGAGTCGATAAGGCCGGCTGACAGCCAGGGAGTGGCCAGCCGGTAATCATTGCCGTCAAGCTGGCGCAATCTTGCCACGTCCGATGCCGTAGTAAGCCAGCCCTGCCGAGGCCATGTGCTCGGGTTCGTACAGGTTGCGGCCATCGAACACCACCCGGTCGGC > NC_002947/3326749‑3327027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |