| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,380,579 | A→G | 10.8% | N70D (AAC→GAC) | tnpT‑I → | cointegrate resolution protein T |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,380,579 | 0 | A | G | 10.8% | 96.8 / 2.7 | 37 | N70D (AAC→GAC) | tnpT‑I | cointegrate resolution protein T |
| Reads supporting (aligned to +/- strand): ref base A (17/16); new base G (2/2); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
CGGGGCGAGAACCCAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG > NC_002947/3380432‑3380727 | cgGGGCGAGAACCCAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAAc < 2:258822/150‑1 (MQ=255)aggggggAAAACCCAAGCATCGATGCGGTACGGGTCGAACTTGGCAATAACTGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAAc < 2:188246/149‑1 (MQ=255) gagaACCCAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTc > 1:261119/1‑150 (MQ=255) gaACCCAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCgg < 1:319665/150‑1 (MQ=255) ccAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCGACCAGGTTGGCCaa < 1:133394/150‑1 (MQ=255) ccAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCGACCAGGTTGGCCaa > 2:133394/1‑150 (MQ=255) cAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAAc > 1:787604/1‑150 (MQ=255) gTACGGGTCGAACTTGGCAATACCGGCTCGAGAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTa > 1:749727/1‑150 (MQ=255) ttGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATgcgc > 1:383216/1‑150 (MQ=255) ggCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAATAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGTAGATCAGCTAG‑AGGAGGATGCGCAg < 1:394707/150‑1 (MQ=255) ccGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCg < 2:261119/150‑1 (MQ=255) ccGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCg > 1:423989/1‑150 (MQ=255) cTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGc > 1:94934/1‑150 (MQ=255) gAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCa > 1:527949/1‑150 (MQ=255) caccaTTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGACAAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAgcg > 1:446250/1‑150 (MQ=255) caccaTTCACCGCTACCTGAAAGAACTCGAAAACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAgcg > 2:99240/1‑150 (MQ=255) ccGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACt > 2:459926/1‑150 (MQ=255) aGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAATCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGaa > 1:92914/1‑148 (MQ=255) aGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAATCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGaa < 2:92914/148‑1 (MQ=255) gAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACgg < 2:383216/150‑1 (MQ=255) tCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCg > 2:95117/1‑150 (MQ=255) tCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCg > 1:32321/1‑150 (MQ=255) gAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGAt > 2:202443/1‑150 (MQ=255) gACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTAc < 2:423989/150‑1 (MQ=255) cgGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGc > 1:108031/1‑150 (MQ=255) cGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGCCCAACCTGGTCGGCCAACTGGTAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGACCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCgg < 1:607526/150‑1 (MQ=255) tACCGCCGCGATACCGCTCCGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGc < 2:749727/150‑1 (MQ=255) tACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGaa < 2:108031/150‑1 (MQ=255) ccGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCAAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATc < 2:94934/150‑1 (MQ=255) gCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCca < 1:249709/150‑1 (MQ=255) aGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAgcgc > 2:723480/1‑81 (MQ=255) aGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAgcgc < 1:723480/81‑1 (MQ=255) gTTGGCCGACCAGGTTGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCca > 1:207845/1‑138 (MQ=255) gTTGGCCGACCAGGTTGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCca < 2:207845/138‑1 (MQ=255) ggCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCa < 2:32321/150‑1 (MQ=255) ccAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCaga < 1:202443/150‑1 (MQ=255) cAACCTGGTCGGCCAACTGGCAGATCAGCTAGAAAGAGG‑GGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCagag > 1:585467/1‑150 (MQ=255) | CGGGGCGAGAACCCAAGCATCGACGCGGTACGGGTCGAACTTGGCAATACCGGCTCGAAAACCACCATTCACCGCTACCTGAAAGAACTCGAAGACGCCGAGCGCGGCCGAAATACCGCCGCGATACCGCTCAGCGAACAGTTGGCCAACCTGGTCGGCCAACTGGCAGATCAGCTAG‑AGGAGGATGCGCAGGCCACCGTGGCCCAGGAGCGCGAACAACTACAACGCGAACGGCTCGATTACCAGAACCAAGCTCGGCTGGCCGAAAGCCGAATCCAGCAGTTGGAAAGTCAGAG > NC_002947/3380432‑3380727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |