Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,435,966 T→C 8.9% L279L (TTG→CTG)  gpJ → baseplate/tail fiber protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,435,9660TC 8.9% 162.5 / 5.1 56L279L (TTG→CTG) gpJbaseplate/tail fiber protein
Reads supporting (aligned to +/- strand):  ref base T (24/27);  new base C (2/3);  total (26/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CCGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAGGTGGTGACATGAG  >  NC_002947/3435821‑3436113
                                                                                                                                                 |                                                                                                                                                   
ccGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGcc                                                                                                                                                 >  2:307663/1‑150 (MQ=255)
       gccgccGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTgcag                                                                                                                                          <  2:426585/150‑4 (MQ=255)
       gccgccGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCg                                                                                                                                          <  2:506472/150‑1 (MQ=255)
       gccgccGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCGACAGGGGCAACTGCCCCTgcag                                                                                                                                          <  1:173660/150‑4 (MQ=255)
        ccgccgGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCgg                                                                                                                                         >  1:502401/1‑150 (MQ=255)
          gccgGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGcc                                                                                                                                       >  1:471802/1‑150 (MQ=255)
                     gACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGccc                                                                                                                                                <  1:731693/130‑1 (MQ=255)
                     gACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGccc                                                                                                                                                >  2:731693/1‑130 (MQ=255)
                      aCTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCTGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCcgcg                                                                                                                           >  2:414211/1‑150 (MQ=255)
                        tGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGtt                                                                                                                         >  1:487192/1‑150 (MQ=255)
                           gTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTaca                                                                                                                      <  2:502401/150‑1 (MQ=255)
                            ttCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTacac                                                                                                                     <  2:471802/150‑1 (MQ=255)
                               aaGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTa                                                                                                                  >  1:234980/1‑150 (MQ=255)
                                 ggTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAAc                                                                                                                <  1:639366/150‑1 (MQ=255)
                                 ggTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCGACAGGGGCAACTGCCCCTgcagg                                                                                                                                         <  2:532702/125‑5 (MQ=255)
                                 ggTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCGACAGGGGCAACTGCCCCTgcagg                                                                                                                                         >  1:532702/1‑121 (MQ=255)
                                                   gATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTa                                                                                                     >  1:343226/1‑143 (MQ=255)
                                                   gATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTa                                                                                                     <  2:343226/143‑1 (MQ=255)
                                                   gATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGAc                                                                                              >  2:541462/1‑150 (MQ=255)
                                                       gAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAATGGGGATTCGTAGCGTGACCTTg                                                                                          <  1:307663/150‑1 (MQ=255)
                                                        aGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGa                                                                                         >  1:780554/1‑150 (MQ=255)
                                                         ggAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGaa                                                                                        <  2:83613/150‑1 (MQ=255)
                                                         ggAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGaa                                                                                        >  2:632893/1‑150 (MQ=255)
                                                         ggAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGaa                                                                                        <  1:423215/150‑1 (MQ=255)
                                                             gggCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGt                                                                                                 <  2:479522/137‑1 (MQ=255)
                                                             gggCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGt                                                                                                 >  1:479522/1‑137 (MQ=255)
                                                                ccAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCa                                                                                 >  2:148988/1‑150 (MQ=255)
                                                                 cAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAg                                                                                >  1:307169/1‑150 (MQ=255)
                                                                          aCGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCgg                                                                         >  1:40720/1‑148 (MQ=255)
                                                                          aCGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCgg                                                                         <  2:40720/148‑1 (MQ=255)
                                                                            gtggtgAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGa                                                                                                           <  2:78377/112‑1 (MQ=255)
                                                                            gtggtgAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGa                                                                                                           >  1:78377/1‑112 (MQ=255)
                                                                            gtggtgAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGggtg                                                                     <  1:541462/150‑1 (MQ=255)
                                                                             tggtgAAGGCCACGGCGCTGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGggtgg                                                                    <  1:414211/150‑1 (MQ=255)
                                                                               gtgAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGgtggtg                                                                  >  2:574582/1‑150 (MQ=255)
                                                                                tgAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGgtgt                                                                 <  1:291513/150‑1 (MQ=255)
                                                                                  aaGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGgtgtgt                                                               <  1:574582/150‑1 (MQ=255)
                                                                                  aaGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGgtgtgt                                                               <  2:487192/150‑1 (MQ=255)
                                                                                    ggCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCGGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGa                                                             <  1:634230/150‑1 (MQ=255)
                                                                                      ccACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATa                                                           <  2:780554/150‑1 (MQ=255)
                                                                                            cgcgGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGCTGTGTTGTGATAAGCGGc                                                     <  2:234980/150‑1 (MQ=255)
                                                                                              cgGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCAt                                                   <  1:148988/150‑1 (MQ=255)
                                                                                                       cTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGt                                          >  1:545981/1‑150 (MQ=255)
                                                                                                         gAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGcc                                                                                                                                       >  2:583608/1‑55 (MQ=255)
                                                                                                         gAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGcc                                                                                                                                       <  1:583608/55‑1 (MQ=255)
                                                                                                                            gCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACg                     >  1:782324/1‑150 (MQ=255)
                                                                                                                              ccGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGggt                                                                      >  2:514755/1‑99 (MQ=255)
                                                                                                                              ccGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGggt                                                                      <  1:514755/99‑1 (MQ=255)
                                                                                                                                 tCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCgcc                <  2:733396/150‑3 (MQ=255)
                                                                                                                                     cgacAGGGGCAACTGCCCCTGTCGGCCATCTACGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTAc                            >  1:243667/4‑134 (MQ=255)
                                                                                                                                     cgacAGGGGCAACTGCCCCTGTCGGCCATCTACGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTAc                            <  2:243667/131‑1 (MQ=255)
                                                                                                                                     cTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAggtg            >  1:355641/1‑150 (MQ=255)
                                                                                                                                     cTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAggtg            <  2:782324/150‑1 (MQ=255)
                                                                                                                                       gCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAggtggt          <  2:307169/150‑1 (MQ=255)
                                                                                                                                             gCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAGGTGGTGACATg    >  1:271556/1‑150 (MQ=255)
                                                                                                                                               aGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGGGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCGGACGTCGAAGGGGGTGGCATGAg  >  1:252105/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCGCGTGGCCGCCGGCGTACCGACTGAGTTCAAGGTGGAGGCCGAGCTGCAGATCGAGGAAGGGCCAGACCCTGACGTGGTGAAGGCCACGGCGCGGGCTGGCCTGAATGCGGCCATTGCAGAAGCCCGTCGCCTGCAGGGGCAGTTGCCCCTGTCGGCCATCTATGCCGCGTTACACGTAACGGGGATTCGTAGCGTGACCTTGAAGCATCCAGCGGCCGGGGTGGTGTGTGATAAGCGGCATTACCCCAGTTGCACGTCGGTTACGCTGACGTCGAAGGTGGTGACATGAG  >  NC_002947/3435821‑3436113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑