| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,688,221 | C→T | 10.3% | L99L (CTG→TTG) | PP_3253 → | carboxylate‑amine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,688,221 | 0 | C | T | 10.3% | 96.7 / 3.9 | 39 | L99L (CTG→TTG) | PP_3253 | carboxylate‑amine ligase |
| Reads supporting (aligned to +/- strand): ref base C (16/19); new base T (2/2); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01 | |||||||||||
AGATCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAAC > NC_002947/3688078‑3688361 | aGATCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCtgctgc < 2:297385/150‑1 (MQ=255) aTCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAg > 2:603720/1‑150 (MQ=255) cGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGaa < 1:603720/150‑1 (MQ=255) ggCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCATCCGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCG‑GGCTTCTGCAGCAGccacgc < 1:685766/150‑5 (MQ=255) aCCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGTCTTCTGCAGAAGCCCGCGGCACAAGGGCACtat < 2:690238/150‑1 (MQ=255) aCCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACtat < 1:285523/150‑1 (MQ=255) ccAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACtata > 1:563860/1‑150 (MQ=255) cTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCATGCGCCGCGTGGCTGCTGCAGACGCCCGCGGCACAAGGGCACTATAagcag > 2:283491/1‑150 (MQ=255) tcCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAg > 1:417957/1‑114 (MQ=255) tcCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAg < 2:417957/114‑1 (MQ=255) ccAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCAtgt > 2:297489/1‑150 (MQ=255) cAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAg < 2:563860/150‑1 (MQ=255) aaCCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCa < 1:460072/110‑1 (MQ=255) aaCCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCa > 2:460072/1‑110 (MQ=255) aaCCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAgg > 2:341729/1‑150 (MQ=255) actgTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGc < 2:293870/147‑1 (MQ=255) ggCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGc < 1:341729/150‑1 (MQ=255) gCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAgg > 2:391639/1‑96 (MQ=255) gCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAgg < 1:391639/96‑1 (MQ=255) tGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCTCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGcc > 1:258454/1‑150 (MQ=255) ggCCGACAAGGGAATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCAtgt < 2:258454/150‑1 (MQ=255) gAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGTTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAagcag < 2:652439/90‑1 (MQ=255) gAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGTTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAagcag < 2:256213/90‑1 (MQ=255) gAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGTTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAagcag > 1:652439/1‑90 (MQ=255) gAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGTTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAagcag > 1:256213/1‑90 (MQ=255) gATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTg < 2:421104/146‑1 (MQ=255) gATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTg > 1:421104/1‑146 (MQ=255) ggCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTATCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTgccgc > 1:406208/1‑150 (MQ=255) aTACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGc > 1:319084/1‑150 (MQ=255) aCGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTc > 2:473706/1‑77 (MQ=255) aCGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTc < 1:473706/77‑1 (MQ=255) gCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTtgt < 1:23568/150‑1 (MQ=255) tGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGa < 2:319084/150‑1 (MQ=255) ccACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGc < 2:450657/106‑1 (MQ=255) ccACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGc > 1:450657/1‑106 (MQ=255) cccTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGGAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTg > 1:299856/1‑150 (MQ=255) cTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTg > 1:573815/1‑121 (MQ=255) cTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTg < 2:573815/121‑1 (MQ=255) gccgcGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATTGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGTAACTGATCAAc < 1:297489/150‑1 (MQ=255) | AGATCGAGCTGGCGTCGCCGGTGTTCACCAACCTGTATGAAGCACGTGAGTTTCTCCAGCGCAACCGTCAGCGGCTGCGCGTGGCATTGGCCGAAGAGGGGATGGGGCCATACGCTGCGGCAAGCCACCCCTGCGCCGCGTGGCTGCTGCAGAAGCCCGCGGCACAAGGGCACTATAAGCAGCTGTTCGACGACTACCGCCATGTGGCAAGGCGTAGCCTGCTCAATGGCCTGCATGTGCACGTTGGCGTGCCGCCAGCTTGTGACCGCATGCAACTGATCAAC > NC_002947/3688078‑3688361 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |