| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,692,662 | T→A | 16.7% | Q336L (CAG→CTG) | PP_3259 ← | acyl‑CoA dehydrogenase‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,692,662 | 0 | T | A | 16.7% | 56.3 / 6.1 | 24 | Q336L (CAG→CTG) | PP_3259 | acyl‑CoA dehydrogenase‑related protein |
| Reads supporting (aligned to +/- strand): ref base T (8/12); new base A (2/2); total (10/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
TCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGAC > NC_002947/3692540‑3692798 | tCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCAGCTGGCCCAgtt < 2:127425/135‑3 (MQ=255)tCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCAGCTGGCCCAgtt > 1:127425/1‑133 (MQ=255) ccAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCg > 2:81010/1‑150 (MQ=255) ccAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCg > 1:347127/1‑150 (MQ=255) ggTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTgg < 1:184896/150‑1 (MQ=255) gTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGc > 2:504024/1‑150 (MQ=255) gcCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCTGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCa < 2:185836/150‑1 (MQ=255) gcTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGc < 2:429151/150‑1 (MQ=255) gCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCa < 1:504024/150‑1 (MQ=255) ccAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCa > 1:726068/1‑133 (MQ=255) ccAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCa < 2:726068/133‑1 (MQ=255) cAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCggg > 2:673996/1‑150 (MQ=255) cGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCaaaa < 1:246381/150‑1 (MQ=255) gcccgcGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCCACATCGCCAGATCACGCTCGTCGTTGCTCTTACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGActg < 1:495455/150‑1 (MQ=255) gACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGAc < 1:673996/150‑1 (MQ=255) gACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGAc < 2:746322/150‑1 (MQ=255) tCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACgg > 1:173611/1‑150 (MQ=255) gTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGAGCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTg < 1:394788/150‑1 (MQ=255) cTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGa < 2:302516/135‑1 (MQ=255) cTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGa > 1:302516/1‑135 (MQ=255) tGGGCCAGCAGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGAc < 1:600873/146‑1 (MQ=255) tGGGCCAGCAGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGAc > 2:600873/1‑146 (MQ=255) ggCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGc > 2:208214/1‑64 (MQ=255) ggCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGc < 1:208214/64‑1 (MQ=255) | TCGAGCCAGGTCGCACGCGCCAGGTGCGCGCTTTGCTGCCAGGCTGCCCAAGGCGTGCCGCCCGCGGACTGGATCAGGTTCTGGCTCATAGTTGCCACACCCCGGCTGGCAACTGGGCCAGCTGCTGGCCCAGCATCGCCAGATCACGCTCGGCGTGGCTCTGACGCAGGAACACCGGCAGGTCGGCGCTCAGCCGGGCAAAATGACTGCTGCGACAGAACGGCGTGGCCCCCAAGGCACGTCCGACGTGGCTTATGAC > NC_002947/3692540‑3692798 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |