| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,760,028 | G→A | 12.5% | L206L (CTG→CTA) ‡ | folEB → | GTP cyclohydrolase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,760,028 | 0 | G | A | 12.5% | 74.9 / 4.2 | 32 | L206L (CTG→CTA) ‡ | folEB | GTP cyclohydrolase I |
| Reads supporting (aligned to +/- strand): ref base G (13/15); new base A (2/2); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.39e-01 | |||||||||||
AGGTCAGGATCGGCTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAGGCGCCTTGCGCACGCCGGTTCAGACCGCCGTAAAACGGGCTGACGAACA > NC_002947/3759880‑3760177 | aGGTCAGGATCGGCTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTTCCCGCAGTTGATTGAGGACGGCTCCATGCGAACCTTCAAGGACCCGACCAC‑CTGCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTgg < 1:418751/150‑1 (MQ=255) tCAGGATCGGCTCCTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGa < 2:545795/150‑1 (MQ=255) gATCGGCTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGcc > 2:691159/1‑150 (MQ=255) gCTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATa < 1:506666/150‑1 (MQ=255) tACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGc > 2:379911/1‑150 (MQ=255) tcctcGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCATGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAATGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACCTAGCCaa > 2:253406/1‑150 (MQ=255) aCCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGc < 2:237648/150‑1 (MQ=255) ccTGCCCGTGCTCGGCCGCCCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGACCCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCg > 1:297702/1‑150 (MQ=255) ggCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCCAGCGACGcg < 1:511546/120‑2 (MQ=255) ggCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCCAGCGACGcg > 2:511546/1‑119 (MQ=255) gCCGCTCTCTCCCGGCAGTGGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACGCG‑TTTTTTATCGCTAAATACTCCCGCGCGCAGTGTCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTgcgc < 1:379911/150‑1 (MQ=255) ctctctCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGc < 1:164958/114‑1 (MQ=255) ctctctCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGc > 2:164958/1‑114 (MQ=255) tcCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTa < 2:338045/150‑1 (MQ=255) ccGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTAcc > 1:563023/1‑150 (MQ=255) gCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGc > 2:522451/1‑150 (MQ=255) gCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGc > 1:670644/1‑150 (MQ=255) aGTTGATTGAGGACGGCTTCCTGTGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCg > 1:306833/1‑150 (MQ=255) attgaGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGcc > 1:62123/1‑150 (MQ=255) gACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCCAGCGACGcgt < 1:476453/93‑3 (MQ=255) gACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCCAGCGACGcgt > 2:476453/1‑91 (MQ=255) gCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCg < 2:793168/150‑1 (MQ=255) aaCCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGc > 1:195693/1‑150 (MQ=255) aCCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGGCCCGGATCTCGGGTTGCTGGcc < 2:297702/150‑1 (MQ=255) cTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTc < 1:790970/98‑1 (MQ=255) cTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTc > 2:790970/1‑98 (MQ=255) ggACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGa < 2:670644/150‑1 (MQ=255) gACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATc > 2:523361/1‑147 (MQ=255) gACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATc < 1:523361/147‑1 (MQ=255) gACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGAc < 1:522451/150‑1 (MQ=255) cTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGaa < 2:306833/150‑1 (MQ=255) gCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAgg < 2:563023/150‑1 (MQ=255) cgcAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCTGTTTGCTGGCCCTGATCGACCACGTCGAAGGCGCCTTGCGCACGCCGGTTc < 2:195693/150‑1 (MQ=255) ggCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAGGCGCCTTGCGCACGCCGGTTCAGACCGCCGTAAAACGGGCTGACGAACa < 2:62123/150‑1 (MQ=255) | AGGTCAGGATCGGCTACTCCTCGACCTGCCCGTGCTCGGCCGCTCTCTCCCGGCAGTTGATTGAGGACGGCTTCCTGCGAACCTTCAAGGACCAG‑CCACTGCTCGAAGTCAGCGCCGTCGCGCAGTGGCTGCGCAGCAACGCGTCGCTGGCGACGCCACATAGCCAACGCAGCGAAGCTCGAGTGCGCGTCACGGTACCTGCCGATGCCCCGGATCTCGGGTTGCTGGCCCTGATCGACCACGTCGAAGGCGCCTTGCGCACGCCGGTTCAGACCGCCGTAAAACGGGCTGACGAACA > NC_002947/3759880‑3760177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |