| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,853,162 | T→A | 21.9% | I169N (ATT→AAT) | alkB → | alpha‑ketoglutarate‑ and Fe(II)‑dependent dioxygenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,853,162 | 0 | T | A | 21.9% | 58.6 / 16.0 | 32 | I169N (ATT→AAT) | alkB | alpha‑ketoglutarate‑ and Fe(II)‑dependent dioxygenase |
| Reads supporting (aligned to +/- strand): ref base T (13/12); new base A (3/4); total (16/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.42e-01 | |||||||||||
TCAACCATTACCTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCGGGTGCCTGAACAGGG > NC_002947/3853015‑3853310 | tCAACCATTACCTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGGCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTTCTGTTCGGCGGCATGCAGCGTGCCGACAAGGCGCAGCGCATTg > 1:644322/1‑150 (MQ=255) aTTACCTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGa < 2:353576/150‑1 (MQ=255) ttACCTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCAATGCGCTGAg < 2:148954/150‑1 (MQ=255) gggCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGa < 2:404202/150‑1 (MQ=255) ggCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGAt > 1:345969/1‑150 (MQ=255) gAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGAGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTg > 2:592516/1‑150 (MQ=255) gCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTggg > 2:520545/1‑150 (MQ=255) gCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTggg > 2:520563/1‑150 (MQ=255) ccTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTgggg > 2:110521/1‑150 (MQ=255) aGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCAATGCGCTGa < 1:208950/102‑1 (MQ=255) aGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCAATGCGCTGa > 2:208950/1‑102 (MQ=255) cGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGt < 1:177060/150‑1 (MQ=255) gTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGc < 2:345969/150‑1 (MQ=255) gATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCAATGCGCTGAGcctt < 1:342793/91‑3 (MQ=255) gATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCAATGCGCTGAGcctt > 2:342793/1‑89 (MQ=255) cggtgtcgCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGCCCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCg < 1:385435/150‑1 (MQ=255) gtgtcgCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGc < 2:65679/150‑1 (MQ=255) gtgtcgCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATCAGCTGGGCCGGCGTTGCGTACACCGCTTGCGCTTCCACGGCGTTCTACCGATCAAACCCGGc > 2:772105/1‑150 (MQ=255) gCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCa > 2:591668/1‑150 (MQ=255) tGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCGGGCTAGCATc > 1:33436/1‑150 (MQ=255) ggcggTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTggg > 1:476441/1‑150 (MQ=255) gcggTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTAc < 2:142373/118‑1 (MQ=255) gcggTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTAc > 1:142373/1‑118 (MQ=255) tGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGa > 2:103175/1‑150 (MQ=255) tGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGa < 1:103175/150‑1 (MQ=255) aCAAGGCTCAGAGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCgg < 1:592516/150‑1 (MQ=255) cAAGGCTCAGCTCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCggg < 1:520563/150‑1 (MQ=255) cAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCGTCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCggg < 1:520545/150‑1 (MQ=255) tCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCGGGTGCCTg > 2:389500/1‑150 (MQ=255) tCAGCGCAATGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAAcggcg > 1:541137/1‑117 (MQ=255) tCAGCGCAATGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAAcggcg < 2:541137/117‑1 (MQ=255) aTTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCGGGTGCCTGAACAggg < 1:591668/150‑1 (MQ=255) | TCAACCATTACCTGCCGGGCACGCGCCTGAGCCTGCACCAGGACCGCGACGAGCAGGATTTCGGTCAGCCGATCGTGTCGGTGTCGCTGGGCTTACCGGCGGTGTTCCTGTTCGGCGGCCTGCAGCGTGCCGACAAGGCTCAGCGCATTGCGCTGAGCCACGGCGATGTGCTGGTCTGGGGTGGCGTAGACCGCTTGCGCTTCCACGGCGTGCTACCGATCAAACCCGGCGTGCATCCGCGCCTGGGCGAACGGCGGATCAACCTGACCTTGCGCAAGGCGGGTGCCTGAACAGGG > NC_002947/3853015‑3853310 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |